Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers

Catecholaminergic (CA) neurons of the medial extended amygdala, preoptic region and adjacent alar hypothalamus have been involved in different aspects of social behavior, as well as in modulation of homeostasis in response to different stressors. Previous data suggested that at least some CA neurons...

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Autores: Morales, Lorena, Desfilis, Ester, Medina Hernández, Loreta Mª
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2025
País:España
Institución:Universitat de Lleida (UdL)
Repositorio:Repositori Obert UdL
OAI Identifier:oai:repositori.udl.cat:10459.1/468671
Acceso en línea:https://doi.org/10.3389/fnana.2025.1553952
https://hdl.handle.net/10459.1/468671
Access Level:acceso abierto
Palabra clave:Catecholamines
Tyrosine hydroxylase
Medial extended amygdala
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spelling Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centersMorales, LorenaDesfilis, EsterMedina Hernández, Loreta MªCatecholaminesTyrosine hydroxylaseMedial extended amygdalaCatecholaminergic (CA) neurons of the medial extended amygdala, preoptic region and adjacent alar hypothalamus have been involved in different aspects of social behavior, as well as in modulation of homeostasis in response to different stressors. Previous data suggested that at least some CA neurons of the medial extended amygdala could originate in a hypothalamic embryonic domain that expresses the transcription factor Otp. To investigate this, we used Otp-eGFP mice (with permanent labeling of GFP in Otp cells) to analyze coexpression of GFP and tyrosine hydroxylase (TH) throughout ontogenesis by way of double immunofluorescence. Our results provide evidence that some forebrain CA cells belong to the Otp lineage. In particular, we found small subpopulations of TH cells that coexpress GFP within the medial extended amygdala, the periventricular preoptic area, the paraventricular hypothalamus, the periventricular hypothalamus, as well as some subdivisions of the basal hypothalamus. In some of the Otp cells, such as those of extended amygdala, the expression of TH appears to be transitory, in agreement with previous studies. The results open interesting questions about the role of these Otp versus non-Otp catecholaminergic subpopulations during development, network integration and in modulation of different functions, including homeostasis and social behaviors.The author(s) declare that financial support was received for the research and/or publication of this article. Funded by the Spanish Ministerio de Ciencia, Innovación y Universidades and Agencia Estatal de Investigación, MICIU/AEI/10.13039/501100011033 and FEDER-EU (Grants no. PID2019-108725RB-100 and no. PID2023- 151927OB-I00), and by the AGAUR/Generalitat de Catalunya (2021 SGR 01359). Lorena Morales had predoctoral contracts from Universitat de Lleida Ajuts Jade Plus, and from IRBLleida/Diputació de Lleida. Currently, Lorena Morales is funded by European Union’s Horizon 2020 Research and Innovation Programme under the INSPIRE COFUND Marie Skłodowska-Curie grant agreement No. 101034270. ED is fellow of the Serra Húnter Senior Programme of the Catalonia Goverment for distinguished professors of Catalan public universities.Frontiers Media2025info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.3389/fnana.2025.1553952https://hdl.handle.net/10459.1/468671reponame:Repositori Obert UdL instname:Universitat de Lleida (UdL)Inglésinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108725RB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151927OB-I00Reproducció del document publicat a https://doi.org/10.3389/fnana.2025.1553952Frontiers in Neuroanatomy, 2025, vol. 19, 1553952cc-by (c) Lorena Morales, Ester Desfilis, Loreta Medina, 2025Attribution 4.0 Internationalinfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/4.0/oai:repositori.udl.cat:10459.1/4686712026-06-24T12:42:17Z
dc.title.none.fl_str_mv Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
title Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
spellingShingle Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
Morales, Lorena
Catecholamines
Tyrosine hydroxylase
Medial extended amygdala
title_short Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
title_full Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
title_fullStr Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
title_full_unstemmed Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
title_sort Development of catecholaminergic neurons of Otp-lineage in the medial extended amygdala and related forebrain centers
dc.creator.none.fl_str_mv Morales, Lorena
Desfilis, Ester
Medina Hernández, Loreta Mª
author Morales, Lorena
author_facet Morales, Lorena
Desfilis, Ester
Medina Hernández, Loreta Mª
author_role author
author2 Desfilis, Ester
Medina Hernández, Loreta Mª
author2_role author
author
dc.subject.none.fl_str_mv Catecholamines
Tyrosine hydroxylase
Medial extended amygdala
topic Catecholamines
Tyrosine hydroxylase
Medial extended amygdala
description Catecholaminergic (CA) neurons of the medial extended amygdala, preoptic region and adjacent alar hypothalamus have been involved in different aspects of social behavior, as well as in modulation of homeostasis in response to different stressors. Previous data suggested that at least some CA neurons of the medial extended amygdala could originate in a hypothalamic embryonic domain that expresses the transcription factor Otp. To investigate this, we used Otp-eGFP mice (with permanent labeling of GFP in Otp cells) to analyze coexpression of GFP and tyrosine hydroxylase (TH) throughout ontogenesis by way of double immunofluorescence. Our results provide evidence that some forebrain CA cells belong to the Otp lineage. In particular, we found small subpopulations of TH cells that coexpress GFP within the medial extended amygdala, the periventricular preoptic area, the paraventricular hypothalamus, the periventricular hypothalamus, as well as some subdivisions of the basal hypothalamus. In some of the Otp cells, such as those of extended amygdala, the expression of TH appears to be transitory, in agreement with previous studies. The results open interesting questions about the role of these Otp versus non-Otp catecholaminergic subpopulations during development, network integration and in modulation of different functions, including homeostasis and social behaviors.
publishDate 2025
dc.date.none.fl_str_mv 2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.3389/fnana.2025.1553952
https://hdl.handle.net/10459.1/468671
url https://doi.org/10.3389/fnana.2025.1553952
https://hdl.handle.net/10459.1/468671
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108725RB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2023-151927OB-I00
Reproducció del document publicat a https://doi.org/10.3389/fnana.2025.1553952
Frontiers in Neuroanatomy, 2025, vol. 19, 1553952
dc.rights.none.fl_str_mv cc-by (c) Lorena Morales, Ester Desfilis, Loreta Medina, 2025
Attribution 4.0 International
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/4.0/
rights_invalid_str_mv cc-by (c) Lorena Morales, Ester Desfilis, Loreta Medina, 2025
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositori Obert UdL
instname:Universitat de Lleida (UdL)
instname_str Universitat de Lleida (UdL)
reponame_str Repositori Obert UdL
collection Repositori Obert UdL
repository.name.fl_str_mv
repository.mail.fl_str_mv
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