Aspergillus niger Secretes Citrate to Increase Iron Bioavailability

Aspergillus niger has an innate ability to secrete various organic acids, including citrate. The conditions required for A. niger citrate overproduction are well described, but the physiological reasons underlying extracellular citrate accumulation are not yet fully understood. One of the less under...

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Autores: Odoni, Dorett I., van Gaal, Merlijn P., Schonewille, Tom, Tamayo Ramos, Juan Antonio, Martins Dos Santos, Vitor A P, Suarez-Diez, Maria, Schaap, Peter J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/414855
Acceso en línea:http://hdl.handle.net/10261/414855
https://api.elsevier.com/content/abstract/scopus_id/85026775856
Access Level:acceso abierto
Palabra clave:Aspergillus niger
Citrate secretion
Iron homeostasis
Metabolic overflow
Siderophores
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spelling Aspergillus niger Secretes Citrate to Increase Iron BioavailabilityOdoni, Dorett I.van Gaal, Merlijn P.Schonewille, TomTamayo Ramos, Juan AntonioMartins Dos Santos, Vitor A PSuarez-Diez, MariaSchaap, Peter J.Aspergillus nigerCitrate secretionIron homeostasisMetabolic overflowSiderophoresAspergillus niger has an innate ability to secrete various organic acids, including citrate. The conditions required for A. niger citrate overproduction are well described, but the physiological reasons underlying extracellular citrate accumulation are not yet fully understood. One of the less understood culture conditions is the requirement of growth-limiting iron concentrations. While this has been attributed to iron-dependent citrate metabolizing enzymes, this straightforward relationship does not always hold true. Here, we show that an increase in citrate secretion under iron limited conditions is a physiological response consistent with a role of citrate as A. niger iron siderophore. We found that A. niger citrate secretion increases with decreasing amounts of iron added to the culture medium and, in contrast to previous findings, this response is independent of the nitrogen source. Differential transcriptomics analyses of the two A. niger mutants NW305 (gluconate non-producer) and NW186 (gluconate and oxalate non-producer) revealed up-regulation of the citrate biosynthesis gene citA under iron limited conditions compared to iron replete conditions. In addition, we show that A. niger can utilize Fe(III) citrate as iron source. Finally, we discuss our findings in the general context of the pH-dependency of A. niger organic acid production, offering an explanation, besides competition, for why A. niger organic acid production is a sequential process influenced by the external pH of the culture medium.WUR IPOP Systems Biology program KB-17-003.02-026 “Genome-wide metabolic modeling and data integration of organic acid production in filamentous fungi.”Peer reviewedFrontiers Media202620262017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/414855https://api.elsevier.com/content/abstract/scopus_id/85026775856reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésFrontiers in microbiologyhttps://doi.org/10.3389/fmicb.2017.01424Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4148552026-05-22T06:33:51Z
dc.title.none.fl_str_mv Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
title Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
spellingShingle Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
Odoni, Dorett I.
Aspergillus niger
Citrate secretion
Iron homeostasis
Metabolic overflow
Siderophores
title_short Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
title_full Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
title_fullStr Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
title_full_unstemmed Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
title_sort Aspergillus niger Secretes Citrate to Increase Iron Bioavailability
dc.creator.none.fl_str_mv Odoni, Dorett I.
van Gaal, Merlijn P.
Schonewille, Tom
Tamayo Ramos, Juan Antonio
Martins Dos Santos, Vitor A P
Suarez-Diez, Maria
Schaap, Peter J.
author Odoni, Dorett I.
author_facet Odoni, Dorett I.
van Gaal, Merlijn P.
Schonewille, Tom
Tamayo Ramos, Juan Antonio
Martins Dos Santos, Vitor A P
Suarez-Diez, Maria
Schaap, Peter J.
author_role author
author2 van Gaal, Merlijn P.
Schonewille, Tom
Tamayo Ramos, Juan Antonio
Martins Dos Santos, Vitor A P
Suarez-Diez, Maria
Schaap, Peter J.
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Aspergillus niger
Citrate secretion
Iron homeostasis
Metabolic overflow
Siderophores
topic Aspergillus niger
Citrate secretion
Iron homeostasis
Metabolic overflow
Siderophores
description Aspergillus niger has an innate ability to secrete various organic acids, including citrate. The conditions required for A. niger citrate overproduction are well described, but the physiological reasons underlying extracellular citrate accumulation are not yet fully understood. One of the less understood culture conditions is the requirement of growth-limiting iron concentrations. While this has been attributed to iron-dependent citrate metabolizing enzymes, this straightforward relationship does not always hold true. Here, we show that an increase in citrate secretion under iron limited conditions is a physiological response consistent with a role of citrate as A. niger iron siderophore. We found that A. niger citrate secretion increases with decreasing amounts of iron added to the culture medium and, in contrast to previous findings, this response is independent of the nitrogen source. Differential transcriptomics analyses of the two A. niger mutants NW305 (gluconate non-producer) and NW186 (gluconate and oxalate non-producer) revealed up-regulation of the citrate biosynthesis gene citA under iron limited conditions compared to iron replete conditions. In addition, we show that A. niger can utilize Fe(III) citrate as iron source. Finally, we discuss our findings in the general context of the pH-dependency of A. niger organic acid production, offering an explanation, besides competition, for why A. niger organic acid production is a sequential process influenced by the external pH of the culture medium.
publishDate 2017
dc.date.none.fl_str_mv 2017
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/414855
https://api.elsevier.com/content/abstract/scopus_id/85026775856
url http://hdl.handle.net/10261/414855
https://api.elsevier.com/content/abstract/scopus_id/85026775856
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in microbiology
https://doi.org/10.3389/fmicb.2017.01424
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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