Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells
Full differentiation potential along with self‐renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro . We show here that transient exposure to a single microRNA, expressed at early stages du...
| Autores: | , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión aceptada para publicación |
| Fecha de publicación: | 2020 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/167804 |
| Acceso en línea: | https://hdl.handle.net/2445/167804 |
| Access Level: | acceso abierto |
| Palabra clave: | Micro RNAs Cèl·lules mare Epigenètica MicroRNAs Stem cells Epigenetics |
| id |
ES_df0d68866835cb3be3a8a282b4e29d23 |
|---|---|
| oai_identifier_str |
oai:diposit.ub.edu:2445/167804 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cellsSalazar-Roa, MaríaTrakala, MariannaÁlvarez Fernández, MónicaValdés Mora, FátimaZhong, CuiqingMuñoz, JaimeYu, YangPeters, Timothy J.Graña Castro, OsvaldoSerrano, RosaZapatero Solana, ElisabetAbad, María LluisaBueno, María JoséGómez de Cedrón, MartaFernández Piqueras, JoséSerrano Marugán, ManuelBlasco, María A.Wang, Da-ZhiClark, Susan J.Izpisúa Belmonte, Juan CarlosOrtega, SagrarioMalumbre, MarcosMicro RNAsCèl·lules mareEpigenèticaMicroRNAsStem cellsEpigeneticsFull differentiation potential along with self‐renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro . We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already‐established murine and human PSCs. Short exposure to miR‐203 in PSCs (mi PSCs) induces a transient expression of 2C markers that later results in expanded differentiation potency to multiple lineages, as well as improved efficiency in tetraploid complementation and human–mouse interspecies chimerism assays. Mechanistically, these effects are at least partially mediated by direct repression of de novo DNA methyltransferases Dnmt3a and Dnmt3b, leading to transient and reversible erasure of DNA methylation. These data support the use of transient exposure to miR‐203 as a versatile method to reset the epigenetic memory in PSCs, and improve their effectiveness in regenerative medicine.EMBO Press2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/167804Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.15252/embj.2019104324The EMBO Journal, 2020, e104324https://doi.org/10.15252/embj.2019104324(c) Salazar Roa et al., 2020info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1678042026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| title |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| spellingShingle |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells Salazar-Roa, María Micro RNAs Cèl·lules mare Epigenètica MicroRNAs Stem cells Epigenetics |
| title_short |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| title_full |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| title_fullStr |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| title_full_unstemmed |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| title_sort |
Transient exposure to miR-203 expands the differentiation capacity of pluripotent stem cells |
| dc.creator.none.fl_str_mv |
Salazar-Roa, María Trakala, Marianna Álvarez Fernández, Mónica Valdés Mora, Fátima Zhong, Cuiqing Muñoz, Jaime Yu, Yang Peters, Timothy J. Graña Castro, Osvaldo Serrano, Rosa Zapatero Solana, Elisabet Abad, María Lluisa Bueno, María José Gómez de Cedrón, Marta Fernández Piqueras, José Serrano Marugán, Manuel Blasco, María A. Wang, Da-Zhi Clark, Susan J. Izpisúa Belmonte, Juan Carlos Ortega, Sagrario Malumbre, Marcos |
| author |
Salazar-Roa, María |
| author_facet |
Salazar-Roa, María Trakala, Marianna Álvarez Fernández, Mónica Valdés Mora, Fátima Zhong, Cuiqing Muñoz, Jaime Yu, Yang Peters, Timothy J. Graña Castro, Osvaldo Serrano, Rosa Zapatero Solana, Elisabet Abad, María Lluisa Bueno, María José Gómez de Cedrón, Marta Fernández Piqueras, José Serrano Marugán, Manuel Blasco, María A. Wang, Da-Zhi Clark, Susan J. Izpisúa Belmonte, Juan Carlos Ortega, Sagrario Malumbre, Marcos |
| author_role |
author |
| author2 |
Trakala, Marianna Álvarez Fernández, Mónica Valdés Mora, Fátima Zhong, Cuiqing Muñoz, Jaime Yu, Yang Peters, Timothy J. Graña Castro, Osvaldo Serrano, Rosa Zapatero Solana, Elisabet Abad, María Lluisa Bueno, María José Gómez de Cedrón, Marta Fernández Piqueras, José Serrano Marugán, Manuel Blasco, María A. Wang, Da-Zhi Clark, Susan J. Izpisúa Belmonte, Juan Carlos Ortega, Sagrario Malumbre, Marcos |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Micro RNAs Cèl·lules mare Epigenètica MicroRNAs Stem cells Epigenetics |
| topic |
Micro RNAs Cèl·lules mare Epigenètica MicroRNAs Stem cells Epigenetics |
| description |
Full differentiation potential along with self‐renewal capacity is a major property of pluripotent stem cells (PSCs). However, the differentiation capacity frequently decreases during expansion of PSCs in vitro . We show here that transient exposure to a single microRNA, expressed at early stages during normal development, improves the differentiation capacity of already‐established murine and human PSCs. Short exposure to miR‐203 in PSCs (mi PSCs) induces a transient expression of 2C markers that later results in expanded differentiation potency to multiple lineages, as well as improved efficiency in tetraploid complementation and human–mouse interspecies chimerism assays. Mechanistically, these effects are at least partially mediated by direct repression of de novo DNA methyltransferases Dnmt3a and Dnmt3b, leading to transient and reversible erasure of DNA methylation. These data support the use of transient exposure to miR‐203 as a versatile method to reset the epigenetic memory in PSCs, and improve their effectiveness in regenerative medicine. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/acceptedVersion |
| format |
article |
| status_str |
acceptedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/167804 |
| url |
https://hdl.handle.net/2445/167804 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Versió postprint del document publicat a: https://doi.org/10.15252/embj.2019104324 The EMBO Journal, 2020, e104324 https://doi.org/10.15252/embj.2019104324 |
| dc.rights.none.fl_str_mv |
(c) Salazar Roa et al., 2020 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
(c) Salazar Roa et al., 2020 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
EMBO Press |
| publisher.none.fl_str_mv |
EMBO Press |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona)) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
| instname_str |
Universidad de Barcelona |
| reponame_str |
Dipòsit Digital de la UB |
| collection |
Dipòsit Digital de la UB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869422029583679488 |
| score |
15,300719 |