Identification of key sequence features required for microRNA biogenesis in plants

Rojas, Arantxa M. L.|||0000-0003-4316-4518; Drusin, Salvador I.|||0000-0001-5350-514X; Chorostecki, Uciel Pablo|||0000-0003-2229-6853; Mateos, Julieta Lisa|||0000-0002-1156-6662; Moro, Belen|||0000-0002-2810-3533; Bologna, Nicolás|||0000-0002-2161-7910; Bresso, Edgardo G.|||0000-0002-9798-459X; Schapire, Arnaldo; Rasia, Rodolfo|||0000-0003-3940-067X; Moreno, Diego M.|||0000-0001-5493-8537; Palatnik, Javier|||0000-0001-7996-5224

Identification of key sequence features required for microRNA biogenesis in plants

MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the...

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Autores:	Rojas, Arantxa M. L.\|\|\|0000-0003-4316-4518, Drusin, Salvador I.\|\|\|0000-0001-5350-514X, Chorostecki, Uciel Pablo\|\|\|0000-0003-2229-6853, Mateos, Julieta Lisa\|\|\|0000-0002-1156-6662, Moro, Belen\|\|\|0000-0002-2810-3533, Bologna, Nicolás\|\|\|0000-0002-2161-7910, Bresso, Edgardo G.\|\|\|0000-0002-9798-459X, Schapire, Arnaldo, Rasia, Rodolfo\|\|\|0000-0003-3940-067X, Moreno, Diego M.\|\|\|0000-0001-5493-8537, Palatnik, Javier\|\|\|0000-0001-7996-5224
Tipo de recurso:	artículo
Fecha de publicación:	2020
País:	España
Institución:	Universitat Autònoma de Barcelona
Repositorio:	Dipòsit Digital de Documents de la UAB
Idioma:	inglés
OAI Identifier:	oai:ddd.uab.cat:237000
Acceso en línea:	https://ddd.uab.cat/record/237000 https://dx.doi.org/urn:doi:10.1038/s41467-020-19129-6
Access Level:	acceso abierto
Palabra clave:	Arabidopsis Arabidopsis Proteins Base Pair Mismatch Cell Cycle Proteins Magnoliopsida MicroRNAs Molecular Dynamics Simulation Nucleic Acid Conformation Polymorphism, Single Nucleotide Ribonuclease III RNA Processing, Post-Transcriptional RNA, Plant

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spelling	Identification of key sequence features required for microRNA biogenesis in plantsRojas, Arantxa M. L.\|\|\|0000-0003-4316-4518Drusin, Salvador I.\|\|\|0000-0001-5350-514XChorostecki, Uciel Pablo\|\|\|0000-0003-2229-6853Mateos, Julieta Lisa\|\|\|0000-0002-1156-6662Moro, Belen\|\|\|0000-0002-2810-3533Bologna, Nicolás\|\|\|0000-0002-2161-7910Bresso, Edgardo G.\|\|\|0000-0002-9798-459XSchapire, ArnaldoRasia, Rodolfo\|\|\|0000-0003-3940-067XMoreno, Diego M.\|\|\|0000-0001-5493-8537Palatnik, Javier\|\|\|0000-0001-7996-5224ArabidopsisArabidopsis ProteinsBase Pair MismatchCell Cycle ProteinsMagnoliopsidaMicroRNAsMolecular Dynamics SimulationNucleic Acid ConformationPolymorphism, Single NucleotideRibonuclease IIIRNA Processing, Post-TranscriptionalRNA, PlantMicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis. 22020-01-0120202020-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/237000https://dx.doi.org/urn:doi:10.1038/s41467-020-19129-6reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2370002026-06-06T12:50:31Z
dc.title.none.fl_str_mv	Identification of key sequence features required for microRNA biogenesis in plants
title	Identification of key sequence features required for microRNA biogenesis in plants
spellingShingle	Identification of key sequence features required for microRNA biogenesis in plants Rojas, Arantxa M. L.\|\|\|0000-0003-4316-4518 Arabidopsis Arabidopsis Proteins Base Pair Mismatch Cell Cycle Proteins Magnoliopsida MicroRNAs Molecular Dynamics Simulation Nucleic Acid Conformation Polymorphism, Single Nucleotide Ribonuclease III RNA Processing, Post-Transcriptional RNA, Plant
title_short	Identification of key sequence features required for microRNA biogenesis in plants
title_full	Identification of key sequence features required for microRNA biogenesis in plants
title_fullStr	Identification of key sequence features required for microRNA biogenesis in plants
title_full_unstemmed	Identification of key sequence features required for microRNA biogenesis in plants
title_sort	Identification of key sequence features required for microRNA biogenesis in plants
dc.creator.none.fl_str_mv	Rojas, Arantxa M. L.\|\|\|0000-0003-4316-4518 Drusin, Salvador I.\|\|\|0000-0001-5350-514X Chorostecki, Uciel Pablo\|\|\|0000-0003-2229-6853 Mateos, Julieta Lisa\|\|\|0000-0002-1156-6662 Moro, Belen\|\|\|0000-0002-2810-3533 Bologna, Nicolás\|\|\|0000-0002-2161-7910 Bresso, Edgardo G.\|\|\|0000-0002-9798-459X Schapire, Arnaldo Rasia, Rodolfo\|\|\|0000-0003-3940-067X Moreno, Diego M.\|\|\|0000-0001-5493-8537 Palatnik, Javier\|\|\|0000-0001-7996-5224
author	Rojas, Arantxa M. L.\|\|\|0000-0003-4316-4518
author_facet	Rojas, Arantxa M. L.\|\|\|0000-0003-4316-4518 Drusin, Salvador I.\|\|\|0000-0001-5350-514X Chorostecki, Uciel Pablo\|\|\|0000-0003-2229-6853 Mateos, Julieta Lisa\|\|\|0000-0002-1156-6662 Moro, Belen\|\|\|0000-0002-2810-3533 Bologna, Nicolás\|\|\|0000-0002-2161-7910 Bresso, Edgardo G.\|\|\|0000-0002-9798-459X Schapire, Arnaldo Rasia, Rodolfo\|\|\|0000-0003-3940-067X Moreno, Diego M.\|\|\|0000-0001-5493-8537 Palatnik, Javier\|\|\|0000-0001-7996-5224
author_role	author
author2	Drusin, Salvador I.\|\|\|0000-0001-5350-514X Chorostecki, Uciel Pablo\|\|\|0000-0003-2229-6853 Mateos, Julieta Lisa\|\|\|0000-0002-1156-6662 Moro, Belen\|\|\|0000-0002-2810-3533 Bologna, Nicolás\|\|\|0000-0002-2161-7910 Bresso, Edgardo G.\|\|\|0000-0002-9798-459X Schapire, Arnaldo Rasia, Rodolfo\|\|\|0000-0003-3940-067X Moreno, Diego M.\|\|\|0000-0001-5493-8537 Palatnik, Javier\|\|\|0000-0001-7996-5224
author2_role	author author author author author author author author author author
dc.subject.none.fl_str_mv	Arabidopsis Arabidopsis Proteins Base Pair Mismatch Cell Cycle Proteins Magnoliopsida MicroRNAs Molecular Dynamics Simulation Nucleic Acid Conformation Polymorphism, Single Nucleotide Ribonuclease III RNA Processing, Post-Transcriptional RNA, Plant
topic	Arabidopsis Arabidopsis Proteins Base Pair Mismatch Cell Cycle Proteins Magnoliopsida MicroRNAs Molecular Dynamics Simulation Nucleic Acid Conformation Polymorphism, Single Nucleotide Ribonuclease III RNA Processing, Post-Transcriptional RNA, Plant
description	MicroRNAs (miRNAs) are endogenous small RNAs of ∼21 nt that regulate multiple biological pathways in multicellular organisms. They derive from longer transcripts that harbor an imperfect stem-loop structure. In plants, the ribonuclease type III DICER-LIKE1 assisted by accessory proteins cleaves the precursor to release the mature miRNA. Numerous studies highlight the role of the precursor secondary structure during plant miRNA biogenesis; however, little is known about the relevance of the precursor sequence. Here, we analyzed the sequence composition of plant miRNA primary transcripts and found specifically located sequence biases. We show that changes in the identity of specific nucleotides can increase or abolish miRNA biogenesis. Most conspicuously, our analysis revealed that the identity of the nucleotides at unpaired positions of the precursor plays a crucial role during miRNA biogenesis in Arabidopsis.
publishDate	2020
dc.date.none.fl_str_mv	2 2020-01-01 2020 2020-01-01
dc.type.none.fl_str_mv	Article http://purl.org/coar/resource_type/c_6501 VoR http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv	info:eu-repo/semantics/article
format	article
dc.identifier.none.fl_str_mv	https://ddd.uab.cat/record/237000 https://dx.doi.org/urn:doi:10.1038/s41467-020-19129-6
url	https://ddd.uab.cat/record/237000 https://dx.doi.org/urn:doi:10.1038/s41467-020-19129-6
dc.language.none.fl_str_mv	Inglés eng
language_invalid_str_mv	Inglés
language	eng
dc.rights.none.fl_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv	info:eu-repo/semantics/openAccess
rights_invalid_str_mv	open access http://purl.org/coar/access_right/c_abf2 https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv	openAccess
dc.format.none.fl_str_mv	application/pdf
dc.source.none.fl_str_mv	reponame:Dipòsit Digital de Documents de la UAB instname:Universitat Autònoma de Barcelona
instname_str	Universitat Autònoma de Barcelona
reponame_str	Dipòsit Digital de Documents de la UAB
collection	Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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Identification of key sequence features required for microRNA biogenesis in plants

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