A versatile luminescent probe for sensing and monitoring amyloid proteins
A modified lysine residue containing an environment-sensitive moiety was prepared through a straightforward synthesis, and its fluorescent properties were examined. The new fluorescent sensor, DMN-BocK, can monitor amyloid aggregation processes associated with neurodegenerative diseases such as Alzh...
| Autores: | , , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad de Barcelona |
| Repositorio: | Dipòsit Digital de la UB |
| OAI Identifier: | oai:diposit.ub.edu:2445/223397 |
| Acceso en línea: | https://hdl.handle.net/2445/223397 |
| Access Level: | acceso abierto |
| Palabra clave: | Proteïnes Malalties neurodegeneratives Amiloides Espectroscòpia de fluorescència Proteins Neurodegenerative Diseases Amyloid Fluorescence spectroscopy |
| id |
ES_dedb2c7e3d1f91f97497a6f367d7dfcb |
|---|---|
| oai_identifier_str |
oai:diposit.ub.edu:2445/223397 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
A versatile luminescent probe for sensing and monitoring amyloid proteinsVázquez Bigas, GuillemEspargaró Colomé, AlbaCaballero Hernández, Ana BelénDi Pede Mattatelli, AniaBusquets i Viñas, Ma. AntoniaNawrot, DariaSabaté Lagunas, RaimonNicolás Galindo, ErnestoJuárez Jiménez, JordiGámez Enamorado, PatrickProteïnesMalalties neurodegenerativesAmiloidesEspectroscòpia de fluorescènciaProteinsNeurodegenerative DiseasesAmyloidFluorescence spectroscopyA modified lysine residue containing an environment-sensitive moiety was prepared through a straightforward synthesis, and its fluorescent properties were examined. The new fluorescent sensor, DMN-BocK, can monitor amyloid aggregation processes associated with neurodegenerative diseases such as Alzheimer’s or Parkinson’s. DMN-BocK offers advantages over classical amyloid-specific dyes like Thioflavins or Congo Red because it is (1) available to detect a broader range of amyloid structures; (2) useful both in vitro and in cellulo; (3) capable of differentiating amyloid structures, providing information on the binding site microenvironment; and (4) a synthon than can be incorporated into protein sequences to gain further structural information. Our findings suggest that DMN-based amino-acid probes have a strong potential to become a sensor of choice for in vitro and in cellulo studies of amyloid aggregation in drug discovery assays.Elsevier B.V.2024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/223397Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1016/j.dyepig.2024.112348Dyes and Pigments, 2024, vol. 231https://doi.org/10.1016/j.dyepig.2024.112348cc-by-nc (c) Vázquez Bigas, Guillem et al., 2024http://creativecommons.org/licenses/by-nc/3.0/es/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/2233972026-05-27T06:46:51Z |
| dc.title.none.fl_str_mv |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| title |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| spellingShingle |
A versatile luminescent probe for sensing and monitoring amyloid proteins Vázquez Bigas, Guillem Proteïnes Malalties neurodegeneratives Amiloides Espectroscòpia de fluorescència Proteins Neurodegenerative Diseases Amyloid Fluorescence spectroscopy |
| title_short |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| title_full |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| title_fullStr |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| title_full_unstemmed |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| title_sort |
A versatile luminescent probe for sensing and monitoring amyloid proteins |
| dc.creator.none.fl_str_mv |
Vázquez Bigas, Guillem Espargaró Colomé, Alba Caballero Hernández, Ana Belén Di Pede Mattatelli, Ania Busquets i Viñas, Ma. Antonia Nawrot, Daria Sabaté Lagunas, Raimon Nicolás Galindo, Ernesto Juárez Jiménez, Jordi Gámez Enamorado, Patrick |
| author |
Vázquez Bigas, Guillem |
| author_facet |
Vázquez Bigas, Guillem Espargaró Colomé, Alba Caballero Hernández, Ana Belén Di Pede Mattatelli, Ania Busquets i Viñas, Ma. Antonia Nawrot, Daria Sabaté Lagunas, Raimon Nicolás Galindo, Ernesto Juárez Jiménez, Jordi Gámez Enamorado, Patrick |
| author_role |
author |
| author2 |
Espargaró Colomé, Alba Caballero Hernández, Ana Belén Di Pede Mattatelli, Ania Busquets i Viñas, Ma. Antonia Nawrot, Daria Sabaté Lagunas, Raimon Nicolás Galindo, Ernesto Juárez Jiménez, Jordi Gámez Enamorado, Patrick |
| author2_role |
author author author author author author author author author |
| dc.subject.none.fl_str_mv |
Proteïnes Malalties neurodegeneratives Amiloides Espectroscòpia de fluorescència Proteins Neurodegenerative Diseases Amyloid Fluorescence spectroscopy |
| topic |
Proteïnes Malalties neurodegeneratives Amiloides Espectroscòpia de fluorescència Proteins Neurodegenerative Diseases Amyloid Fluorescence spectroscopy |
| description |
A modified lysine residue containing an environment-sensitive moiety was prepared through a straightforward synthesis, and its fluorescent properties were examined. The new fluorescent sensor, DMN-BocK, can monitor amyloid aggregation processes associated with neurodegenerative diseases such as Alzheimer’s or Parkinson’s. DMN-BocK offers advantages over classical amyloid-specific dyes like Thioflavins or Congo Red because it is (1) available to detect a broader range of amyloid structures; (2) useful both in vitro and in cellulo; (3) capable of differentiating amyloid structures, providing information on the binding site microenvironment; and (4) a synthon than can be incorporated into protein sequences to gain further structural information. Our findings suggest that DMN-based amino-acid probes have a strong potential to become a sensor of choice for in vitro and in cellulo studies of amyloid aggregation in drug discovery assays. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/223397 |
| url |
https://hdl.handle.net/2445/223397 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: https://doi.org/10.1016/j.dyepig.2024.112348 Dyes and Pigments, 2024, vol. 231 https://doi.org/10.1016/j.dyepig.2024.112348 |
| dc.rights.none.fl_str_mv |
cc-by-nc (c) Vázquez Bigas, Guillem et al., 2024 http://creativecommons.org/licenses/by-nc/3.0/es/ info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by-nc (c) Vázquez Bigas, Guillem et al., 2024 http://creativecommons.org/licenses/by-nc/3.0/es/ |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Elsevier B.V. |
| publisher.none.fl_str_mv |
Elsevier B.V. |
| dc.source.none.fl_str_mv |
Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica) reponame:Dipòsit Digital de la UB instname:Universidad de Barcelona |
| instname_str |
Universidad de Barcelona |
| reponame_str |
Dipòsit Digital de la UB |
| collection |
Dipòsit Digital de la UB |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869422012376547328 |
| score |
15.811543 |