In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides

The microbial colonisation of the infant gut begins immediately after birth and is essential for the development of the intestine, the immune system and later well-being. Important differences have been reported in the characteristics of such microbiota in different infant population groups. In the...

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Autores: Arboleya, Silvia, Salazar, Nuria, Solís, Gonzalo, Fernández, Nuria, Gueimonde Fernández, Miguel, González de los Reyes-Gavilán, Clara
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2013
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/346025
Acceso en línea:http://hdl.handle.net/10261/346025
Access Level:acceso abierto
Palabra clave:Bifidobacterium
Intestinal microbiota
Infant population groups
Probiotics
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spelling In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharidesArboleya, SilviaSalazar, NuriaSolís, GonzaloFernández, NuriaGueimonde Fernández, MiguelGonzález de los Reyes-Gavilán, ClaraBifidobacteriumIntestinal microbiotaInfant population groupsProbioticsThe microbial colonisation of the infant gut begins immediately after birth and is essential for the development of the intestine, the immune system and later well-being. Important differences have been reported in the characteristics of such microbiota in different infant population groups. In the present study, we employed an in vitro faecal batch culture model using faeces from different human population groups (adults and full-term breast-fed, full-term formula-fed and preterm infants) to determine the influence that the addition of four bifidobacterial strains and fructo-oligosaccharides (FOS) exerts on the profile of SCFA measured by GC as well as on the levels of some relevant intestinal microbial groups by quantitative PCR during incubation. Differences were found in the levels of SCFA and intestinal microbial groups in the faecal cultures depending on the human group origin of the faecal samples (P< 0·05), this being a predominant factor, compared with bifidobacteria or FOS added, in determining microbiota dynamics. These results exhibit the importance of the initial characteristics of the basal intestinal microbiota in the effect exerted by bifidobacteria or FOS that are added and highlight the need to design probiotics targeting specific human population groups.The present study was financially supported by European Union FEDER funds and by the Spanish Ministry of Economy and Competitiveness under the project AGL2010-16525 and by CSIC through the project PIE201370E19. S. A. was funded by a predoctoral JAE fellowship from CSIC, Spain.Peer reviewedCambridge University PressEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242013info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttp://hdl.handle.net/10261/346025reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//AGL2010-16525https://doi.org/10.1017/S0007114513001487Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3460252026-05-22T06:33:51Z
dc.title.none.fl_str_mv In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
title In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
spellingShingle In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
Arboleya, Silvia
Bifidobacterium
Intestinal microbiota
Infant population groups
Probiotics
title_short In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
title_full In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
title_fullStr In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
title_full_unstemmed In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
title_sort In vitro evaluation of the impact of human background microbiota on the response to Bifidobacterium strains and fructo-oligosaccharides
dc.creator.none.fl_str_mv Arboleya, Silvia
Salazar, Nuria
Solís, Gonzalo
Fernández, Nuria
Gueimonde Fernández, Miguel
González de los Reyes-Gavilán, Clara
author Arboleya, Silvia
author_facet Arboleya, Silvia
Salazar, Nuria
Solís, Gonzalo
Fernández, Nuria
Gueimonde Fernández, Miguel
González de los Reyes-Gavilán, Clara
author_role author
author2 Salazar, Nuria
Solís, Gonzalo
Fernández, Nuria
Gueimonde Fernández, Miguel
González de los Reyes-Gavilán, Clara
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bifidobacterium
Intestinal microbiota
Infant population groups
Probiotics
topic Bifidobacterium
Intestinal microbiota
Infant population groups
Probiotics
description The microbial colonisation of the infant gut begins immediately after birth and is essential for the development of the intestine, the immune system and later well-being. Important differences have been reported in the characteristics of such microbiota in different infant population groups. In the present study, we employed an in vitro faecal batch culture model using faeces from different human population groups (adults and full-term breast-fed, full-term formula-fed and preterm infants) to determine the influence that the addition of four bifidobacterial strains and fructo-oligosaccharides (FOS) exerts on the profile of SCFA measured by GC as well as on the levels of some relevant intestinal microbial groups by quantitative PCR during incubation. Differences were found in the levels of SCFA and intestinal microbial groups in the faecal cultures depending on the human group origin of the faecal samples (P< 0·05), this being a predominant factor, compared with bifidobacteria or FOS added, in determining microbiota dynamics. These results exhibit the importance of the initial characteristics of the basal intestinal microbiota in the effect exerted by bifidobacteria or FOS that are added and highlight the need to design probiotics targeting specific human population groups.
publishDate 2013
dc.date.none.fl_str_mv 2013
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/346025
url http://hdl.handle.net/10261/346025
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//AGL2010-16525
https://doi.org/10.1017/S0007114513001487
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cambridge University Press
publisher.none.fl_str_mv Cambridge University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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