Dissecting hippocampal circuits underlying cognitive functions
The hippocampus supports navigation and memory, among other cognitive functions. Understanding how the hippocampal circuitry supports these functions is still an open question. In this thesis, I inquired about how hippocampal activity is affected by different cognitive demands, and how specific cell...
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| Formato: | tesis doctoral |
| Fecha de publicación: | 2025 |
| País: | España |
| Recursos: | Universidad Complutense de Madrid (UCM) |
| Repositorio: | Docta Complutense |
| Idioma: | español |
| OAI Identifier: | oai:docta.ucm.es:20.500.14352/119367 |
| Acesso em linha: | https://hdl.handle.net/20.500.14352/119367 |
| Access Level: | acceso abierto |
| Palavra-chave: | 611.813.14(043.2) Hipocampo Ciencias Biomédicas 2410 Biología Humana |
| Resumo: | The hippocampus supports navigation and memory, among other cognitive functions. Understanding how the hippocampal circuitry supports these functions is still an open question. In this thesis, I inquired about how hippocampal activity is affected by different cognitive demands, and how specific cell types in the CA1 -deep and superficial pyramidal neurons- contribute to specific cognitive functions. An automatic multi-purpose maze and cognitive tasks were designed, and prototypical hippocampal activity – sharp-wave ripples and place cells- was recorded using either electrophysiology techniques or calcium imaging approaches. The features of sharp-wvae ripples during awake, but not sleep, were modulated by cognitive demands such as novelty, learning and experience. Place cell activity from deep and superficial pyramidal neurons contributed differently to navigation, where deep neurons were found to be more tightly modulated by local cues and superficial neurons by global cues and context. These results were further explored using chemogenetic manipulations and multiplexed recordings of both neuronal subtypes. Assessing the contribution of these subpopulations to memory functions showed that deep neurons are more strongly modulated by trial segments in maze tasks, and that these subpopulations may contribute differently to task and reward representation. |
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