Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats

El pdf del artículo es la versión pre-print.-- et al.

Detalles Bibliográficos
Autores: Pertusa, María, García-Matas, Silvia, Adell, Albert, Rodrigo Anoro, Teresa, Cristòfol, Rosa, Sanfeliu, Coral
Tipo de recurso: artículo
Fecha de publicación:2008
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:dnet:digitalcsic_::133a28eac68c0a021cbc29dab012949b
Acceso en línea:http://hdl.handle.net/10261/37328
Access Level:acceso abierto
Palabra clave:Glial cell line-derived neurotrophic factor (GDNF)
Lentiviral vector
Aging
Learning and memory
Gene therapy
Acetylcholine
Dopamine
Serotonin
Rat
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spelling Expression of GDNF transgene in astrocytes improves cognitive deficits in aged ratsPertusa, MaríaGarcía-Matas, SilviaAdell, AlbertRodrigo Anoro, TeresaCristòfol, RosaSanfeliu, CoralGlial cell line-derived neurotrophic factor (GDNF)Lentiviral vectorAgingLearning and memoryGene therapyAcetylcholineDopamineSerotoninRatEl pdf del artículo es la versión pre-print.-- et al.Glial cell line-derived neurotrophic factor (GDNF) was assayed for its neurotrophic effects against the neuronal atrophy that causes cognitive deficits in old age. Aged Fisher 344 rats with impairment in the Morris water maze received intrahippocampal injections at the dorsal CA1 area of either a lentiviral vector encoding human GDNF or the same vector encoding human green fluorescent protein as a control. Recombinant lentiviral vectors constructed with human cytomegalovirus promotor and pseudotyped with lyssavirus Mokola glycoprotein specifically transduced the astrocytes in vivo. Astrocyte-secreted GDNF enhanced neuron function as shown by local increases in synthesis of the neurotransmitters acetylcholine, dopamine and serotonin. This neurotrophic effect led to cognitive improvement of the rats as early as 2 weeks after gene transduction. Spatial learning and memory testing showed a significant gain in cognitive abilities due to GDNF exposure, whereas control-transduced rats kept their performance at the chance level. These results confirm the broad spectrum of the neurotrophic action of GDNF and open new gene therapy possibilities for reducing age-related neurodegeneration.Elsevier201120112008info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/37328reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1016/j.neurobiolaging.2007.02.026info:eu-repo/semantics/openAccessoai:dnet:digitalcsic_::133a28eac68c0a021cbc29dab012949b2026-05-22T06:33:51Z
dc.title.none.fl_str_mv Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
title Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
spellingShingle Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
Pertusa, María
Glial cell line-derived neurotrophic factor (GDNF)
Lentiviral vector
Aging
Learning and memory
Gene therapy
Acetylcholine
Dopamine
Serotonin
Rat
title_short Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
title_full Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
title_fullStr Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
title_full_unstemmed Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
title_sort Expression of GDNF transgene in astrocytes improves cognitive deficits in aged rats
dc.creator.none.fl_str_mv Pertusa, María
García-Matas, Silvia
Adell, Albert
Rodrigo Anoro, Teresa
Cristòfol, Rosa
Sanfeliu, Coral
author Pertusa, María
author_facet Pertusa, María
García-Matas, Silvia
Adell, Albert
Rodrigo Anoro, Teresa
Cristòfol, Rosa
Sanfeliu, Coral
author_role author
author2 García-Matas, Silvia
Adell, Albert
Rodrigo Anoro, Teresa
Cristòfol, Rosa
Sanfeliu, Coral
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Glial cell line-derived neurotrophic factor (GDNF)
Lentiviral vector
Aging
Learning and memory
Gene therapy
Acetylcholine
Dopamine
Serotonin
Rat
topic Glial cell line-derived neurotrophic factor (GDNF)
Lentiviral vector
Aging
Learning and memory
Gene therapy
Acetylcholine
Dopamine
Serotonin
Rat
description El pdf del artículo es la versión pre-print.-- et al.
publishDate 2008
dc.date.none.fl_str_mv 2008
2011
2011
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/37328
url http://hdl.handle.net/10261/37328
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1016/j.neurobiolaging.2007.02.026
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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