Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women

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Detalles Bibliográficos
Autores: Muñoz Bobo, Marina, Camargo, Milena, Soto de León, Sara, Sánchez, Ricardo, Pineda Peña, Andrea, Pérez Prados, Antonio, Patarroyo, Manuel Elkin, Patarroyo, Manuel Alfonso
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2013
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/23536
Acceso en línea:https://hdl.handle.net/2454/23536
Access Level:acceso abierto
Palabra clave:Human papillomavirus (HPV)
HIV
PCR-based moleculat tests
Urine samples
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spelling Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected womenMuñoz Bobo, MarinaCamargo, MilenaSoto de León, SaraSánchez, RicardoPineda Peña, AndreaPérez Prados, AntonioPatarroyo, Manuel ElkinPatarroyo, Manuel AlfonsoHuman papillomavirus (HPV)HIVPCR-based moleculat testsUrine samplesIncluye un fichero de datosHuman papillomavirus (HPV) is the main risk factor associated with the development of cervical cancer (CC); however, there are other factors, such as immunosuppression caused by the human immunodeficiency virus (HIV), that favor progression of the illness. This study was thus aimed at evaluating the functionality of classical PCR-based molecular tests for the generic identification of HPV DNA (GP5 /GP6 , MY09/MY11, and pU1M/2R primers, individually or in combination) using cervical and urine samples from 194 HIV-positive women. Infected samples were tested with type-specific primers for six high-risk types (HPV-16, -18, -31, -33, -45, and -58) and two low-risk types (HPV-6 and -11). HPV infection prevalence rates were 70.1% for the cervical samples and 63.9% for the urine samples. HPV-16 was the most prevalent viral type in the cervical and urine samples, with higher rates of multiple infections than single infections detected in such samples. HPV DNA detection by PCR (mainly with the pU1M/2R primer set) in urine samples was positively associated with abnormal cytological findings (atypical squamous cells of undetermined significance/squamous intraepithelial lesions [ASCUS/SIL]). It was determined that the operative characteristics for detection of cytological abnormalities were similar for cervical and urine samples. This suggested using PCR for the detection of HPV DNA in urine samples as a potential screening strategy for CC prevention in future prevention and control programs along with currently implemented strategies for reducing the impact of the disease, i.e., urine samples are economical, are easy to collect, have wide acceptability among women, and have operative characteristics similar to those of cervical samples.The authors are grateful to the Basque Cooperation Agency for Development and the Spanish Agency for International Development Cooperation (AECID) for supporting and financing this project.American Society for MicrobiologyEstadística e Investigación OperativaEstatistika eta Ikerketa Operatiboa2013info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/ziphttps://hdl.handle.net/2454/23536reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglés© 2013 American Society for Microbiology. All Rights Reserved.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/235362026-06-17T12:41:47Z
dc.title.none.fl_str_mv Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
spellingShingle Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
Muñoz Bobo, Marina
Human papillomavirus (HPV)
HIV
PCR-based moleculat tests
Urine samples
title_short Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_full Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_fullStr Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_full_unstemmed Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
title_sort Classical molecular tests using urine samples as a potential screening tool for human papillomavirus detection in human immunodeficiency virus-infected women
dc.creator.none.fl_str_mv Muñoz Bobo, Marina
Camargo, Milena
Soto de León, Sara
Sánchez, Ricardo
Pineda Peña, Andrea
Pérez Prados, Antonio
Patarroyo, Manuel Elkin
Patarroyo, Manuel Alfonso
author Muñoz Bobo, Marina
author_facet Muñoz Bobo, Marina
Camargo, Milena
Soto de León, Sara
Sánchez, Ricardo
Pineda Peña, Andrea
Pérez Prados, Antonio
Patarroyo, Manuel Elkin
Patarroyo, Manuel Alfonso
author_role author
author2 Camargo, Milena
Soto de León, Sara
Sánchez, Ricardo
Pineda Peña, Andrea
Pérez Prados, Antonio
Patarroyo, Manuel Elkin
Patarroyo, Manuel Alfonso
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Estadística e Investigación Operativa
Estatistika eta Ikerketa Operatiboa
dc.subject.none.fl_str_mv Human papillomavirus (HPV)
HIV
PCR-based moleculat tests
Urine samples
topic Human papillomavirus (HPV)
HIV
PCR-based moleculat tests
Urine samples
description Incluye un fichero de datos
publishDate 2013
dc.date.none.fl_str_mv 2013
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/23536
url https://hdl.handle.net/2454/23536
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv © 2013 American Society for Microbiology. All Rights Reserved.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2013 American Society for Microbiology. All Rights Reserved.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/zip
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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