Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies

Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a...

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Autores: Medel-Martínez, Ana, Redrado-Osta, Ana, Crespo-Barreda, Alejandra, Sancho-Albero, Maria, Sánchez, Lourdes, Sebastián, Víctor, Pardo, María, De la Vieja, Antonio, Martin-Duque, Pilar
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Instituto de Salud Carlos III (ISCIII)
Repositorio:Repisalud
Idioma:inglés
OAI Identifier:oai:repisalud.isciii.es:20.500.12105/25530
Acceso en línea:https://hdl.handle.net/20.500.12105/25530
Access Level:acceso abierto
Palabra clave:Casein
Characterization
Extraction protocol
Milk exosomes
Milk fat globules
Rennet
Storage
Animals
Cattle
Chymosin
Exosomes
Glycolipids
Glycoproteins
Humans
Lipid Droplets
Milk
Milk, Human
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repository_id_str
spelling Isolation and Characterization of Milk Exosomes for Use in Advanced TherapiesMedel-Martínez, AnaRedrado-Osta, AnaCrespo-Barreda, AlejandraSancho-Albero, MariaSánchez, LourdesSebastián, VíctorPardo, MaríaDe la Vieja, AntonioMartin-Duque, PilarCaseinCharacterizationExtraction protocolMilk exosomesMilk fat globulesRennetStorageAnimalsCattleChymosinExosomesGlycolipidsGlycoproteinsHumansLipid DropletsMilkMilk, HumanExosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk-exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.Multidisciplinary Digital Publishing Institute (MDPI)Instituto de Salud Carlos IIIAsociación de Padres de Niños Oncológicos de AragónAsociación Española Contra el CáncerUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)Ministerio de Ciencia e Innovación (España)Fundación Ramón Areces20242024-11-1920242024-07-0820242024-07-08research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.12105/25530reponame:Repisaludinstname:Instituto de Salud Carlos III (ISCIII)InglésengInstituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII) PI19%2F01007 USO DEL SIMPORTER DE YODO Y SODIO (HNIS) ENDOGENO EN EXOSOMAS DE PLACENTA, COMO HERRAMIENTA PARA LA TERAPIA Y DIAGNOSTICO DE CANCER. ENDOEXONISInstituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII) DTS21%2F00130 ANDAMIOS POROSOS FUNCIONALIZADOS CON EXOSOMAS COMO HERRAMIENTA DE CAPTURA DE CELULAS TUMORALES (TC-CAP)ES RTI2018-099343-B-100 Not availableES PID2021-125948OB-I00 Not availableopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repisalud.isciii.es:20.500.12105/255302026-06-12T12:43:37Z
dc.title.none.fl_str_mv Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
title Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
spellingShingle Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
Medel-Martínez, Ana
Casein
Characterization
Extraction protocol
Milk exosomes
Milk fat globules
Rennet
Storage
Animals
Cattle
Chymosin
Exosomes
Glycolipids
Glycoproteins
Humans
Lipid Droplets
Milk
Milk, Human
title_short Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
title_full Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
title_fullStr Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
title_full_unstemmed Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
title_sort Isolation and Characterization of Milk Exosomes for Use in Advanced Therapies
dc.creator.none.fl_str_mv Medel-Martínez, Ana
Redrado-Osta, Ana
Crespo-Barreda, Alejandra
Sancho-Albero, Maria
Sánchez, Lourdes
Sebastián, Víctor
Pardo, María
De la Vieja, Antonio
Martin-Duque, Pilar
author Medel-Martínez, Ana
author_facet Medel-Martínez, Ana
Redrado-Osta, Ana
Crespo-Barreda, Alejandra
Sancho-Albero, Maria
Sánchez, Lourdes
Sebastián, Víctor
Pardo, María
De la Vieja, Antonio
Martin-Duque, Pilar
author_role author
author2 Redrado-Osta, Ana
Crespo-Barreda, Alejandra
Sancho-Albero, Maria
Sánchez, Lourdes
Sebastián, Víctor
Pardo, María
De la Vieja, Antonio
Martin-Duque, Pilar
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto de Salud Carlos III
Asociación de Padres de Niños Oncológicos de Aragón
Asociación Española Contra el Cáncer
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
Ministerio de Ciencia e Innovación (España)
Fundación Ramón Areces
dc.subject.none.fl_str_mv Casein
Characterization
Extraction protocol
Milk exosomes
Milk fat globules
Rennet
Storage
Animals
Cattle
Chymosin
Exosomes
Glycolipids
Glycoproteins
Humans
Lipid Droplets
Milk
Milk, Human
topic Casein
Characterization
Extraction protocol
Milk exosomes
Milk fat globules
Rennet
Storage
Animals
Cattle
Chymosin
Exosomes
Glycolipids
Glycoproteins
Humans
Lipid Droplets
Milk
Milk, Human
description Exosomes are cell-derived extracellular vesicles (EVs) with diameters between 30 and 120 nm. In recent years, several studies have evaluated the therapeutic potential of exosomes derived from different fluids due to their low immunogenicity and high biocompatibility. However, producing exosomes on a large scale is still challenging. One of the fluids from which they could be isolated in large quantities is milk. Moreover, regeneration is a well-known property of milk. The present work seeks to optimize a method for isolating exosomes from bovine and human milk, comparing different storage conditions and different extraction protocols. We found differences in the yield extraction associated with pre-storage milk conditions and observed some differences according to the processing agent. When we removed milk fat globules and added rennet before freezing, we obtained a cleaner final fraction. In summary, we attempted to optimize a rennet-based new milk-exosome isolation method and concluded that pre-treatment, followed by freezing of samples, yielded the best exosome population.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-11-19
2024
2024-07-08
2024
2024-07-08
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.12105/25530
url https://hdl.handle.net/20.500.12105/25530
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII) PI19%2F01007 USO DEL SIMPORTER DE YODO Y SODIO (HNIS) ENDOGENO EN EXOSOMAS DE PLACENTA, COMO HERRAMIENTA PARA LA TERAPIA Y DIAGNOSTICO DE CANCER. ENDOEXONIS
Instituto de Salud Carlos III http://dx.doi.org/10.13039/501100004587 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 (ISCIII) DTS21%2F00130 ANDAMIOS POROSOS FUNCIONALIZADOS CON EXOSOMAS COMO HERRAMIENTA DE CAPTURA DE CELULAS TUMORALES (TC-CAP)
ES RTI2018-099343-B-100 Not available
ES PID2021-125948OB-I00 Not available
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute (MDPI)
dc.source.none.fl_str_mv reponame:Repisalud
instname:Instituto de Salud Carlos III (ISCIII)
instname_str Instituto de Salud Carlos III (ISCIII)
reponame_str Repisalud
collection Repisalud
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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