Manipulation of cancer cell spheroids by photovoltaic tweezers: Determination of their charge state
Photovoltaic optoelectronic tweezers (PVOT), based on the bulk photovoltaiceffect presented by certain ferroelectric crystals, have emerged as a versatile toolto manipulate and/or trap micro- and nano-objects. Very recently, an ingeniousmethod has enabled to extend PVOT manipulation to aqueous solut...
| Autores: | , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2024 |
| País: | España |
| Institución: | Universidad Autónoma de Madrid |
| Repositorio: | Biblos-e Archivo. Repositorio Institucional de la UAM |
| Idioma: | inglés |
| OAI Identifier: | oai:repositorio.uam.es:10486/715863 |
| Acceso en línea: | http://hdl.handle.net/10486/715863 https://dx.doi.org/10.1002/adpr.202400124 |
| Access Level: | acceso abierto |
| Palabra clave: | Droplet Optofluidics Ferroelectric Platforms Multicellular Spheroids Optoelectronic Manipulations Photovoltaic Tweezers Física |
| Sumario: | Photovoltaic optoelectronic tweezers (PVOT), based on the bulk photovoltaiceffect presented by certain ferroelectric crystals, have emerged as a versatile toolto manipulate and/or trap micro- and nano-objects. Very recently, an ingeniousmethod has enabled to extend PVOT manipulation to aqueous solution dropletsopening the door to applications in optofluidics and biotechnology. The pho-tovoltaic crystal is placed on the bottom of a cuvette filled with paraffin oil whilethe droplet is hanging from the air–oil interphase where it is manipulated.Herein, this method is used to investigate the feasibility of manipulating hybriddroplets as carriers containing a cancer multicellular spheroid inside cell culturemedium. Droplets of two cancer cell lines, MCF-7 and U-87 MG, have beensuccessfully manipulated by light-induced electrical fields generated in thephotovoltaic platform. From the droplet dynamics, which includes attractive andrepulsive behaviors from the illuminated crystal region, the charge state of thecell culture medium and the spheroid are investigated. The results show that theyare positively and negatively charged, respectively. Moreover, analyzing themigration kinetics of water, cell culture medium, and hybrid droplets, to the lightof a proposed theoretical model, the charge density of both cell line spheroids hasbeen estimated |
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