Comparative transcriptome and histomorphology analysis of skeletal muscle in Fujian white rabbit (Oryctolagus cuniculus) at different growth stages
[EN] Transcriptome sequencing was performed to analyse the differentially expressed genes (DEGs) of skeletal muscle in Fujian white rabbits at different stages, to help establish gene expression datasets for further investigation of the mechanisms underlying genetic skeletal muscle growth in Fujian...
| Autores: | , , , , , , , |
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| Tipo de recurso: | artículo |
| Fecha de publicación: | 2025 |
| País: | España |
| Institución: | Universitat Politècnica de València (UPV) |
| Repositorio: | RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia |
| Idioma: | inglés |
| OAI Identifier: | oai:riunet.upv.es:10251/231845 |
| Acceso en línea: | https://riunet.upv.es/handle/10251/231845 |
| Access Level: | acceso abierto |
| Palabra clave: | Fujian white rabbit Transcriptome Skeletal muscle development Gene expression Growth stage Muscle fiber |
| Sumario: | [EN] Transcriptome sequencing was performed to analyse the differentially expressed genes (DEGs) of skeletal muscle in Fujian white rabbits at different stages, to help establish gene expression datasets for further investigation of the mechanisms underlying genetic skeletal muscle growth in Fujian white rabbit. The transcriptomes of skeletal muscle in Fujian white rabbits at five growth stages with 25 rabbits (5 rabbits per stage) were analysed. Double fluorescence staining was performed on skeletal muscle sections at three postnatal stages for muscle fibre classification and statistical analysis. A total of 9737 DEGs were acquired from five stages. The Kyoto Encyclopaedia of Genes and Genomes analysis showed that 8249 genes were enriched in 1148 pathways, of which 67 were significantly (P<0.05) enriched, mainly in the mitogen-activated protein kinase signalling pathway, glucagon signalling pathway and peroxisome proliferator-activated receptors signalling pathway associated with normal skeletal muscle development. Among all groups, skipped exons were the most abundant alternative splice event. Many of the DEGs were well known to be related to growth of skeletal muscle in Fujian white rabbits including myogenic factor 5, Myomesin-2, Phosphoglycerate mutase 2, Myostatin and Myogenic factor 6, which were verified by quantitative reverse transcription polymerase chain reaction. These results contribute to define the molecular regulatory mechanism of muscle growth and development in Fujian white rabbits and serve as a theoretical basis for improving the meat production and growth of meat rabbit breeds. |
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