Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide

Introduction: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the dia...

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Autores: Riveros, Ana L., Eggeling, Cynthia, Riquelme, Sebastián, Adura, Carolina, López-Iglesias, Carmen, Guzmán, Fanny, Araya, Eyleen, Almada, Mario, Juárez, Josué, Váldez, Miguel Angel, Fuentevilla, Ignacio Andrés, López Serrano, Olga, Kogan, Marcelo J.
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/213940
Acesso em linha:http://hdl.handle.net/10261/213940
Access Level:Acceso aberto
Palavra-chave:Gold nanorots
Cell-penetrating peptides
Liposome
Amphipathic arginine rich peptide
Biological barrier permeation
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spelling Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptideRiveros, Ana L.Eggeling, CynthiaRiquelme, SebastiánAdura, CarolinaLópez-Iglesias, CarmenGuzmán, FannyAraya, EyleenAlmada, MarioJuárez, JosuéVáldez, Miguel AngelFuentevilla, Ignacio AndrésLópez Serrano, OlgaKogan, Marcelo J.Gold nanorotsCell-penetrating peptidesLiposomeAmphipathic arginine rich peptideBiological barrier permeationIntroduction: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD. Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM. Results and Discussion: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage. Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.This research was funded by Fondecyt Postdoctoral 3130654; FONDAP 15130011; Fondecyt 1170929, Fondecyt 1190623, Nucleo UNAB 36/18N, CONACYT/CONICYT (México) 204393; CONICYT/CONACYT (Chile), and Fondequip EQM170111.Peer reviewedDove PressConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/213940reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.2147/IJN.S237820Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2139402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
title Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
spellingShingle Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
Riveros, Ana L.
Gold nanorots
Cell-penetrating peptides
Liposome
Amphipathic arginine rich peptide
Biological barrier permeation
title_short Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
title_full Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
title_fullStr Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
title_full_unstemmed Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
title_sort Improving cell penetration of gold nanorods by using an amphipathic arginine rich peptide
dc.creator.none.fl_str_mv Riveros, Ana L.
Eggeling, Cynthia
Riquelme, Sebastián
Adura, Carolina
López-Iglesias, Carmen
Guzmán, Fanny
Araya, Eyleen
Almada, Mario
Juárez, Josué
Váldez, Miguel Angel
Fuentevilla, Ignacio Andrés
López Serrano, Olga
Kogan, Marcelo J.
author Riveros, Ana L.
author_facet Riveros, Ana L.
Eggeling, Cynthia
Riquelme, Sebastián
Adura, Carolina
López-Iglesias, Carmen
Guzmán, Fanny
Araya, Eyleen
Almada, Mario
Juárez, Josué
Váldez, Miguel Angel
Fuentevilla, Ignacio Andrés
López Serrano, Olga
Kogan, Marcelo J.
author_role author
author2 Eggeling, Cynthia
Riquelme, Sebastián
Adura, Carolina
López-Iglesias, Carmen
Guzmán, Fanny
Araya, Eyleen
Almada, Mario
Juárez, Josué
Váldez, Miguel Angel
Fuentevilla, Ignacio Andrés
López Serrano, Olga
Kogan, Marcelo J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Gold nanorots
Cell-penetrating peptides
Liposome
Amphipathic arginine rich peptide
Biological barrier permeation
topic Gold nanorots
Cell-penetrating peptides
Liposome
Amphipathic arginine rich peptide
Biological barrier permeation
description Introduction: Gold nanorods are highly reactive, have a large surface-to-volume ratio, and can be functionalized with biomolecules. Gold nanorods can absorb infrared electromagnetic radiation, which is subsequently dispersed as local heat. Gold nanoparticles can be used as powerful tools for the diagnosis and therapy of different diseases. To improve the biological barrier permeation of nanoparticles with low cytotoxicity, in this study, we conjugated gold nanorods with cell-penetrating peptides (oligoarginines) and with the amphipathic peptide CLPFFD. Methods: We studied the interaction of the functionalized gold nanorods with biological membrane models (liposomes) by dynamic light scattering, transmission electron microscopy and the Langmuir balance. Furthermore, we evaluated the effects on cell viability and permeability with an MTS assay and TEM. Results and Discussion: The interaction study by DLS, the Langmuir balance and cryo-TEM support that GNR-Arg7CLPFFD enhances the interactions between GNRs and biological membranes. In addition, cells treated with GNR-Arg7CLPFFD internalized 80% more nanoparticles than cells treated with GNR alone and did not induce cell damage. Conclusion: Our results indicate that incorporation of an amphipathic sequence into oligoarginines for the functionalization of gold nanorods enhances biological membrane nanoparticle interactions and nanoparticle cell permeability with respect to nanorods functionalized with oligoarginine. Overall, functionalized gold nanorods with amphipathic arginine rich peptides might be candidates for improving drug delivery by facilitating biological barrier permeation.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/213940
url http://hdl.handle.net/10261/213940
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.2147/IJN.S237820
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Dove Press
publisher.none.fl_str_mv Dove Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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