An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness
The impact of global warming on transpiration and photosynthesis would compromise plant fitness, impacting on crop yields and ecosystem functioning. In this frame, we explored the performance of a set of Arabidopsis mutants carrying partial or total loss-of-function alleles of stomatal development g...
| Autores: | , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2022 |
| País: | España |
| Institución: | Universidad de Castilla-La Mancha |
| Repositorio: | RUIdeRA. Repositorio Institucional de la UCLM |
| OAI Identifier: | oai:ruidera.uclm.es:10578/46496 |
| Acceso en línea: | https://www.frontiersin.org/articles/10.3389/fpls.2022.919299/full https://hdl.handle.net/10578/46496 |
| Access Level: | acceso abierto |
| Palabra clave: | Autofluorescence imaging Heat adaptation Photosynthesis Stomatal development Supra-optimal temperature Thermomorphogenesis Transpiration |
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An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thicknessPérez Bueno, María LuisaIllescas Miranda, JonatanFernández Martín-Forero, Amanda Leticiade Marcos Serrano, AlbertoBarón Ayala, MatildeFenoll Comes, María CarmenMena Marugan, María de la MontañaAutofluorescence imagingHeat adaptationPhotosynthesisStomatal developmentSupra-optimal temperatureThermomorphogenesisTranspirationThe impact of global warming on transpiration and photosynthesis would compromise plant fitness, impacting on crop yields and ecosystem functioning. In this frame, we explored the performance of a set of Arabidopsis mutants carrying partial or total loss-of-function alleles of stomatal development genes and displaying distinct stomatal abundances. Using microscopy and non-invasive imaging techniques on this genotype collection, we examined anatomical leaf and stomatal traits, plant growth and development, and physiological performance at optimal (22°C) and supra-optimal (30°C) temperatures. All genotypes showed thermomorphogenetic responses but no signs of heat stress. Data analysis singled out an extremely low stomatal abundance mutant, spch-5. At 22°C, spch-5 had lower transpiration and warmer leaves than the wild type. However, at 30°C, this mutant developed larger stomata and thinner leaves, paralleled by a notable cooling capacity, similar to that of the wild type. Despite their low stomatal density (SD), spch-5 plants grown at 30°C showed no photosynthesis or growth penalties. The behavior of spch-5 at supra-optimal temperature exemplifies how the effect of very low stomatal numbers can be counteracted by a combination of larger stomata and thinner leaves. Furthermore, it provides a novel strategy for coping with high growth temperatures.Frontiers Media202620262022info:eu-repo/semantics/articleapplication/pdfapplication/pdfhttps://www.frontiersin.org/articles/10.3389/fpls.2022.919299/fullhttps://hdl.handle.net/10578/46496reponame:RUIdeRA. Repositorio Institucional de la UCLMinstname:Universidad de Castilla-La ManchaInglésinfo:eu-repo/semantics/openAccessoai:ruidera.uclm.es:10578/464962026-05-27T07:36:41Z |
| dc.title.none.fl_str_mv |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| title |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| spellingShingle |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness Pérez Bueno, María Luisa Autofluorescence imaging Heat adaptation Photosynthesis Stomatal development Supra-optimal temperature Thermomorphogenesis Transpiration |
| title_short |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| title_full |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| title_fullStr |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| title_full_unstemmed |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| title_sort |
An extremely low stomatal density mutant overcomes cooling limitations at supra-optimal temperature by adjusting stomatal size and leaf thickness |
| dc.creator.none.fl_str_mv |
Pérez Bueno, María Luisa Illescas Miranda, Jonatan Fernández Martín-Forero, Amanda Leticia de Marcos Serrano, Alberto Barón Ayala, Matilde Fenoll Comes, María Carmen Mena Marugan, María de la Montaña |
| author |
Pérez Bueno, María Luisa |
| author_facet |
Pérez Bueno, María Luisa Illescas Miranda, Jonatan Fernández Martín-Forero, Amanda Leticia de Marcos Serrano, Alberto Barón Ayala, Matilde Fenoll Comes, María Carmen Mena Marugan, María de la Montaña |
| author_role |
author |
| author2 |
Illescas Miranda, Jonatan Fernández Martín-Forero, Amanda Leticia de Marcos Serrano, Alberto Barón Ayala, Matilde Fenoll Comes, María Carmen Mena Marugan, María de la Montaña |
| author2_role |
author author author author author author |
| dc.subject.none.fl_str_mv |
Autofluorescence imaging Heat adaptation Photosynthesis Stomatal development Supra-optimal temperature Thermomorphogenesis Transpiration |
| topic |
Autofluorescence imaging Heat adaptation Photosynthesis Stomatal development Supra-optimal temperature Thermomorphogenesis Transpiration |
| description |
The impact of global warming on transpiration and photosynthesis would compromise plant fitness, impacting on crop yields and ecosystem functioning. In this frame, we explored the performance of a set of Arabidopsis mutants carrying partial or total loss-of-function alleles of stomatal development genes and displaying distinct stomatal abundances. Using microscopy and non-invasive imaging techniques on this genotype collection, we examined anatomical leaf and stomatal traits, plant growth and development, and physiological performance at optimal (22°C) and supra-optimal (30°C) temperatures. All genotypes showed thermomorphogenetic responses but no signs of heat stress. Data analysis singled out an extremely low stomatal abundance mutant, spch-5. At 22°C, spch-5 had lower transpiration and warmer leaves than the wild type. However, at 30°C, this mutant developed larger stomata and thinner leaves, paralleled by a notable cooling capacity, similar to that of the wild type. Despite their low stomatal density (SD), spch-5 plants grown at 30°C showed no photosynthesis or growth penalties. The behavior of spch-5 at supra-optimal temperature exemplifies how the effect of very low stomatal numbers can be counteracted by a combination of larger stomata and thinner leaves. Furthermore, it provides a novel strategy for coping with high growth temperatures. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022 2026 2026 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://www.frontiersin.org/articles/10.3389/fpls.2022.919299/full https://hdl.handle.net/10578/46496 |
| url |
https://www.frontiersin.org/articles/10.3389/fpls.2022.919299/full https://hdl.handle.net/10578/46496 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Frontiers Media |
| publisher.none.fl_str_mv |
Frontiers Media |
| dc.source.none.fl_str_mv |
reponame:RUIdeRA. Repositorio Institucional de la UCLM instname:Universidad de Castilla-La Mancha |
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Universidad de Castilla-La Mancha |
| reponame_str |
RUIdeRA. Repositorio Institucional de la UCLM |
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RUIdeRA. Repositorio Institucional de la UCLM |
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15,811543 |