Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218

Ibuprofen is one of the most common drugs found as a contaminant in soils, sediments, and waters. Although several microorganisms able to metabolize ibuprofen have been described, the metabolic pathways and factors limiting biodegradation in nature remain poorly characterized. Among the bacteria abl...

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Autores: Aulestia, Magaly, Flores, Amando, Acosta-Jurado, Sebastián, Santero, Eduardo, Camacho, Eva
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Pablo de Olavide (UPO)
Repositorio:RIO. Repositorio Institucional Olavide
Idioma:inglés
OAI Identifier:oai:rio.upo.es:10433/25230
Acesso em linha:https://hdl.handle.net/10433/25230
Access Level:acceso abierto
Palavra-chave:Biodegradation pathway
Biodegradative plasmid
Emerging contaminant
Genetics
Ibuprofen
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spelling Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218Aulestia, MagalyFlores, AmandoAcosta-Jurado, SebastiánSantero, EduardoCamacho, EvaBiodegradation pathwayBiodegradative plasmidEmerging contaminantGeneticsIbuprofenIbuprofen is one of the most common drugs found as a contaminant in soils, sediments, and waters. Although several microorganisms able to metabolize ibuprofen have been described, the metabolic pathways and factors limiting biodegradation in nature remain poorly characterized. Among the bacteria able to grow on ibuprofen, three different strains belonging to Sphingomonadaceae and isolated from different geographical locations carry the same set of genes required for the upper part of the ibuprofen metabolic pathway. Here, we have studied the metabolic pathway of Rhizorhabdus wittichii MPO218, identifying new genes required for the lower part of the ibuprofen metabolic pathway. We have identified two new DNA regions in MPO218 involved in the metabolism of ibuprofen. One is located on the MPO218 chromosome and appears to be required for the metabolism of propionyl-CoA through the methylmalonyl-CoA pathway. Although involved in ibuprofen metabolism, this region is not strictly necessary for growing using ibuprofen. The second region belongs to the pIBU218 plasmid and comprises two gene clusters containing aromatic compound biodegradation genes, part of which are necessary for ibuprofen degradation. We have identified two genes required for the first two steps of the lower part of the ibuprofen metabolic pathway (ipfL and ipfM), and, based on our results, we propose the putative complete pathway for ibuprofen metabolism in strain MPO218. IMPORTANCE Ibuprofen, one of the most common pharmaceutical contaminants in natural environments, is toxic for some aquatic and terrestrial organisms. The main source of environmental ibuprofen is wastewater, so improving wastewater treatment is of relevant importance. Although several microorganisms capable of biodegrading ibuprofen have been described, the metabolic pathways and their genetic bases remain poorly understood. Three bacterial strains of the family Sphingomonadaceae capable of using ibuprofen as carbon and energy source have been described. Although the genes involved in the upper part of the degradation pathway (ipfABDEF cluster) have been identified, those required for the lower part of the pathway remained unknown. Here, we have confirmed the requirement of the ipf cluster for the generation of isobutyl catechol and have identified the genes involved in the subsequent transformation of the metabolic products. Identification of genes involved in ibuprofen degradation is essential to developing improved strains for the removal of this contaminant.ASM20252025-12-1620222022-01-0120222022-01-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10433/25230reponame:RIO. Repositorio Institucional Olavideinstname:Universidad Pablo de Olavide (UPO)InglésengMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available BIO2014-57545-R MODELOS DE REGULACION GLOBAL Y ESPECIFICA EN BACTERIAS DEGRADADORAS DE CONTAMINANTES AMBIENTALESopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:rio.upo.es:10433/252302026-06-13T12:46:27Z
dc.title.none.fl_str_mv Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
title Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
spellingShingle Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
Aulestia, Magaly
Biodegradation pathway
Biodegradative plasmid
Emerging contaminant
Genetics
Ibuprofen
title_short Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
title_full Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
title_fullStr Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
title_full_unstemmed Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
title_sort Genetic Characterization of the Ibuprofen-Degradative Pathway of Rhizorhabdus wittichii MPO218
dc.creator.none.fl_str_mv Aulestia, Magaly
Flores, Amando
Acosta-Jurado, Sebastián
Santero, Eduardo
Camacho, Eva
author Aulestia, Magaly
author_facet Aulestia, Magaly
Flores, Amando
Acosta-Jurado, Sebastián
Santero, Eduardo
Camacho, Eva
author_role author
author2 Flores, Amando
Acosta-Jurado, Sebastián
Santero, Eduardo
Camacho, Eva
author2_role author
author
author
author
dc.contributor.none.fl_str_mv
dc.subject.none.fl_str_mv Biodegradation pathway
Biodegradative plasmid
Emerging contaminant
Genetics
Ibuprofen
topic Biodegradation pathway
Biodegradative plasmid
Emerging contaminant
Genetics
Ibuprofen
description Ibuprofen is one of the most common drugs found as a contaminant in soils, sediments, and waters. Although several microorganisms able to metabolize ibuprofen have been described, the metabolic pathways and factors limiting biodegradation in nature remain poorly characterized. Among the bacteria able to grow on ibuprofen, three different strains belonging to Sphingomonadaceae and isolated from different geographical locations carry the same set of genes required for the upper part of the ibuprofen metabolic pathway. Here, we have studied the metabolic pathway of Rhizorhabdus wittichii MPO218, identifying new genes required for the lower part of the ibuprofen metabolic pathway. We have identified two new DNA regions in MPO218 involved in the metabolism of ibuprofen. One is located on the MPO218 chromosome and appears to be required for the metabolism of propionyl-CoA through the methylmalonyl-CoA pathway. Although involved in ibuprofen metabolism, this region is not strictly necessary for growing using ibuprofen. The second region belongs to the pIBU218 plasmid and comprises two gene clusters containing aromatic compound biodegradation genes, part of which are necessary for ibuprofen degradation. We have identified two genes required for the first two steps of the lower part of the ibuprofen metabolic pathway (ipfL and ipfM), and, based on our results, we propose the putative complete pathway for ibuprofen metabolism in strain MPO218. IMPORTANCE Ibuprofen, one of the most common pharmaceutical contaminants in natural environments, is toxic for some aquatic and terrestrial organisms. The main source of environmental ibuprofen is wastewater, so improving wastewater treatment is of relevant importance. Although several microorganisms capable of biodegrading ibuprofen have been described, the metabolic pathways and their genetic bases remain poorly understood. Three bacterial strains of the family Sphingomonadaceae capable of using ibuprofen as carbon and energy source have been described. Although the genes involved in the upper part of the degradation pathway (ipfABDEF cluster) have been identified, those required for the lower part of the pathway remained unknown. Here, we have confirmed the requirement of the ipf cluster for the generation of isobutyl catechol and have identified the genes involved in the subsequent transformation of the metabolic products. Identification of genes involved in ibuprofen degradation is essential to developing improved strains for the removal of this contaminant.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-01-01
2022
2022-01-01
2025
2025-12-16
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10433/25230
url https://hdl.handle.net/10433/25230
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 Not available BIO2014-57545-R MODELOS DE REGULACION GLOBAL Y ESPECIFICA EN BACTERIAS DEGRADADORAS DE CONTAMINANTES AMBIENTALES
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv ASM
publisher.none.fl_str_mv ASM
dc.source.none.fl_str_mv reponame:RIO. Repositorio Institucional Olavide
instname:Universidad Pablo de Olavide (UPO)
instname_str Universidad Pablo de Olavide (UPO)
reponame_str RIO. Repositorio Institucional Olavide
collection RIO. Repositorio Institucional Olavide
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812429

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