The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum

All multicellular organisms, including ascidians, host diverse microbial communities that are essential for their evolution. The global invader Didemnum vexillum is a colonial species native to Japan with two main genetic clades, A (the only invasive) and B, which provides a unique opportunity to as...

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Detalles Bibliográficos
Autores: Casso, María, Turon, Marta, Marco, Núria, Pascual Berniola, Marta, Turon Barrera, Xavier
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/175770
Acceso en línea:https://hdl.handle.net/2445/175770
Access Level:acceso abierto
Palabra clave:Simbiosi
Microbiota
Espècies introduïdes
Ascidiacis
Symbiosis
Introduced organisms
Sea squirts
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spelling The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillumCasso, MaríaTuron, MartaMarco, NúriaPascual Berniola, MartaTuron Barrera, XavierSimbiosiMicrobiotaEspècies introduïdesAscidiacisSymbiosisMicrobiotaIntroduced organismsSea squirtsAll multicellular organisms, including ascidians, host diverse microbial communities that are essential for their evolution. The global invader Didemnum vexillum is a colonial species native to Japan with two main genetic clades, A (the only invasive) and B, which provides a unique opportunity to assess if the microbiome remains stable in the colonization process or shifts according to local environment. We have analyzed, using 16S amplicon sequencing, the microbiome of 65 D. vexillum colonies from 13 populations worldwide including the two clades in the native area, plus samples from a congeneric species and seawater from one of the localities. We found 3,525 zero-radius operational taxonomic units (ZOTUs) in D. vexillum, belonging to 36 bacterial and 3 archaeal phyla. The microbiome of this species had a markedly different composition from surrounding seawater and from the congeneric species. For the globally invasive clade A, we found 3,154 ZOTUs, and 8 of them were present in all colonies, constituting a core microbiome with high abundance (69.57% of the total reads) but low diversity (0.25% of the total number of ZOTUs). The variable component was quantitatively much less important but comprised a highly diverse assemblage. In a multiple regression model, global microbiome structure correlated with differences in temperature range across localities and also with geographic distances, pointing to horizontal acquisition of the symbionts. However, the ascidian may have a strong capacity to select and enrich its microbiome, as we found that the most abundant ZOTUs from tunic samples had low abundance in seawater samples from the same locality. The microbiome structure also correlated with the genetic distances between colonies obtained in a previous genome-wide analysis, suggesting some potential for vertical transmission. In geographically restricted comparisons, temperature and genetic makeup, but not geography, explained microbiome structure. The combination of a quantitatively dominant core component and a highly diverse variable fraction in the microbiome of D. vexillum can contribute to the success of this global invader in different environments.Frontiers Media2020info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/175770Articles publicats en revistes (Genètica, Microbiologia i Estadística)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3389/fmars.2020.00201Frontiers In Marine Science, 2020, vol. 7https://doi.org/10.3389/fmars.2020.00201cc-by (c) Casso, María et al., 2020http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1757702026-05-27T06:46:51Z
dc.title.none.fl_str_mv The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
title The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
spellingShingle The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
Casso, María
Simbiosi
Microbiota
Espècies introduïdes
Ascidiacis
Symbiosis
Microbiota
Introduced organisms
Sea squirts
title_short The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
title_full The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
title_fullStr The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
title_full_unstemmed The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
title_sort The Microbiome of the Worldwide Invasive Ascidian Didemnum vexillum
dc.creator.none.fl_str_mv Casso, María
Turon, Marta
Marco, Núria
Pascual Berniola, Marta
Turon Barrera, Xavier
author Casso, María
author_facet Casso, María
Turon, Marta
Marco, Núria
Pascual Berniola, Marta
Turon Barrera, Xavier
author_role author
author2 Turon, Marta
Marco, Núria
Pascual Berniola, Marta
Turon Barrera, Xavier
author2_role author
author
author
author
dc.subject.none.fl_str_mv Simbiosi
Microbiota
Espècies introduïdes
Ascidiacis
Symbiosis
Microbiota
Introduced organisms
Sea squirts
topic Simbiosi
Microbiota
Espècies introduïdes
Ascidiacis
Symbiosis
Microbiota
Introduced organisms
Sea squirts
description All multicellular organisms, including ascidians, host diverse microbial communities that are essential for their evolution. The global invader Didemnum vexillum is a colonial species native to Japan with two main genetic clades, A (the only invasive) and B, which provides a unique opportunity to assess if the microbiome remains stable in the colonization process or shifts according to local environment. We have analyzed, using 16S amplicon sequencing, the microbiome of 65 D. vexillum colonies from 13 populations worldwide including the two clades in the native area, plus samples from a congeneric species and seawater from one of the localities. We found 3,525 zero-radius operational taxonomic units (ZOTUs) in D. vexillum, belonging to 36 bacterial and 3 archaeal phyla. The microbiome of this species had a markedly different composition from surrounding seawater and from the congeneric species. For the globally invasive clade A, we found 3,154 ZOTUs, and 8 of them were present in all colonies, constituting a core microbiome with high abundance (69.57% of the total reads) but low diversity (0.25% of the total number of ZOTUs). The variable component was quantitatively much less important but comprised a highly diverse assemblage. In a multiple regression model, global microbiome structure correlated with differences in temperature range across localities and also with geographic distances, pointing to horizontal acquisition of the symbionts. However, the ascidian may have a strong capacity to select and enrich its microbiome, as we found that the most abundant ZOTUs from tunic samples had low abundance in seawater samples from the same locality. The microbiome structure also correlated with the genetic distances between colonies obtained in a previous genome-wide analysis, suggesting some potential for vertical transmission. In geographically restricted comparisons, temperature and genetic makeup, but not geography, explained microbiome structure. The combination of a quantitatively dominant core component and a highly diverse variable fraction in the microbiome of D. vexillum can contribute to the success of this global invader in different environments.
publishDate 2020
dc.date.none.fl_str_mv 2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/175770
url https://hdl.handle.net/2445/175770
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3389/fmars.2020.00201
Frontiers In Marine Science, 2020, vol. 7
https://doi.org/10.3389/fmars.2020.00201
dc.rights.none.fl_str_mv cc-by (c) Casso, María et al., 2020
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Casso, María et al., 2020
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Articles publicats en revistes (Genètica, Microbiologia i Estadística)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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