Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model

Pregnant BALB/c mice have been widely used as an in vivo model to study Neospora caninum infection biology and to provide proof-of-concept for assessments of drugs and vaccines against neosporosis. The fact that this model has been used with different isolates of variable virulence, varying infectio...

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Detalles Bibliográficos
Autores: Arranz Solís, David, Aguado-Martínez, Adriana, Müller, Joachim, Ortega Mora, Luis Miguel, Regidor-Cerrillo, Javier, Hemphill, Andrew
Tipo de recurso: artículo
Fecha de publicación:2015
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/91545
Acceso en línea:https://hdl.handle.net/20.500.14352/91545
Access Level:acceso abierto
Palabra clave:Neospora caninum, Neosporosis, Pregnant mouse model, Nc-spain7, Experimental infection, Dose titration
Parasitología (Veterinaria)
3109 Ciencias Veterinarias
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spelling Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse modelArranz Solís, DavidAguado-Martínez, AdrianaMüller, JoachimOrtega Mora, Luis MiguelRegidor-Cerrillo, JavierHemphill, AndrewNeospora caninum, Neosporosis, Pregnant mouse model, Nc-spain7, Experimental infection, Dose titrationParasitología (Veterinaria)3109 Ciencias VeterinariasPregnant BALB/c mice have been widely used as an in vivo model to study Neospora caninum infection biology and to provide proof-of-concept for assessments of drugs and vaccines against neosporosis. The fact that this model has been used with different isolates of variable virulence, varying infection routes and differing methods to prepare the parasites for infection, has rendered the comparison of results from different laboratories impossible. In most studies, mice were infected with similar number of parasites (2×106) as employed in ruminant models (107 for cows and 106 for sheep), which seems inappropriate considering the enormous differences in the weight of these species. Thus, for achieving meaningful results in vaccination and drug efficacy experiments, a refinement and standardization of this experimental model is necessary. Thus, 2×106, 105, 104, 103 and 102 tachyzoites of the highly virulent and well-characterised Nc-Spain7 isolate were subcutaneously inoculated into mice at day 7 of pregnancy, and clinical outcome, vertical transmission, parasite burden and antibody responses were compared. Dams from all infected groups presented nervous signs and the percentage of surviving pups at day 30 postpartum was surprisingly low (24%) in mice infected with only 102 tachyzoites. Importantly, infection with 105 tachyzoites resulted in antibody levels, cerebral parasite burden in dams and 100% mortality rate in pups, which was identical to infection with 2×106 tachyzoites. Considering these results, it is reasonable to lower the challenge dose to 105 tachyzoites in further experiments when assessing drugs or vaccine candidatesElsevierUniversidad Complutense de Madrid20152015-01-0120152015-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/91545reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/915452026-06-02T12:44:21Z
dc.title.none.fl_str_mv Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
title Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
spellingShingle Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
Arranz Solís, David
Neospora caninum, Neosporosis, Pregnant mouse model, Nc-spain7, Experimental infection, Dose titration
Parasitología (Veterinaria)
3109 Ciencias Veterinarias
title_short Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
title_full Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
title_fullStr Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
title_full_unstemmed Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
title_sort Dose-dependent effects of experimental infection with the virulent Neospora caninum Nc-Spain7 isolate in a pregnant mouse model
dc.creator.none.fl_str_mv Arranz Solís, David
Aguado-Martínez, Adriana
Müller, Joachim
Ortega Mora, Luis Miguel
Regidor-Cerrillo, Javier
Hemphill, Andrew
author Arranz Solís, David
author_facet Arranz Solís, David
Aguado-Martínez, Adriana
Müller, Joachim
Ortega Mora, Luis Miguel
Regidor-Cerrillo, Javier
Hemphill, Andrew
author_role author
author2 Aguado-Martínez, Adriana
Müller, Joachim
Ortega Mora, Luis Miguel
Regidor-Cerrillo, Javier
Hemphill, Andrew
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv Neospora caninum, Neosporosis, Pregnant mouse model, Nc-spain7, Experimental infection, Dose titration
Parasitología (Veterinaria)
3109 Ciencias Veterinarias
topic Neospora caninum, Neosporosis, Pregnant mouse model, Nc-spain7, Experimental infection, Dose titration
Parasitología (Veterinaria)
3109 Ciencias Veterinarias
description Pregnant BALB/c mice have been widely used as an in vivo model to study Neospora caninum infection biology and to provide proof-of-concept for assessments of drugs and vaccines against neosporosis. The fact that this model has been used with different isolates of variable virulence, varying infection routes and differing methods to prepare the parasites for infection, has rendered the comparison of results from different laboratories impossible. In most studies, mice were infected with similar number of parasites (2×106) as employed in ruminant models (107 for cows and 106 for sheep), which seems inappropriate considering the enormous differences in the weight of these species. Thus, for achieving meaningful results in vaccination and drug efficacy experiments, a refinement and standardization of this experimental model is necessary. Thus, 2×106, 105, 104, 103 and 102 tachyzoites of the highly virulent and well-characterised Nc-Spain7 isolate were subcutaneously inoculated into mice at day 7 of pregnancy, and clinical outcome, vertical transmission, parasite burden and antibody responses were compared. Dams from all infected groups presented nervous signs and the percentage of surviving pups at day 30 postpartum was surprisingly low (24%) in mice infected with only 102 tachyzoites. Importantly, infection with 105 tachyzoites resulted in antibody levels, cerebral parasite burden in dams and 100% mortality rate in pups, which was identical to infection with 2×106 tachyzoites. Considering these results, it is reasonable to lower the challenge dose to 105 tachyzoites in further experiments when assessing drugs or vaccine candidates
publishDate 2015
dc.date.none.fl_str_mv 2015
2015-01-01
2015
2015-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/91545
url https://hdl.handle.net/20.500.14352/91545
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
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