Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583

Enterococcus faecalis is one of the most controversial species of lactic acid bacteria. Some strains are used as probiotics, while others are associated with severe and life-threatening nosocomial infections. Their pathogenicity depends on the acqui-sition of multidrug resistance and virulence facto...

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Autores: Pérez, Marta M., Calles-Enríquez, Marina, Río Lagar, Beatriz del, Ladero Losada, Víctor Manuel, Martín, M. Cruz, Fernández García, María, Álvarez González, Miguel Ángel
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2015
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/143039
Acceso en línea:http://hdl.handle.net/10261/143039
Access Level:acceso abierto
Palabra clave:Biofilms
Gelatinase
Insertion sequences
Enterococcus faecalis
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spelling Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583Pérez, Marta M.Calles-Enríquez, MarinaRío Lagar, Beatriz delLadero Losada, Víctor ManuelMartín, M. CruzFernández García, MaríaÁlvarez González, Miguel ÁngelBiofilmsGelatinaseInsertion sequencesEnterococcus faecalisEnterococcus faecalis is one of the most controversial species of lactic acid bacteria. Some strains are used as probiotics, while others are associated with severe and life-threatening nosocomial infections. Their pathogenicity depends on the acqui-sition of multidrug resistance and virulence factors. Gelatinase, which is required in the first steps of biofilm formation, is an important virulence determinant involved in E. faecalis pathogenesis, including endocarditis and peritonitis. The gene that codes for gelatinase (gelE) is controlled by the Fsr quorum-sensing system, whose encoding genes (fsrA, fsrB, fsrC, and fsrD) are located immediately upstream of gelE. The integration of a DNA fragment into the fsr locus of a derived mutant of E. faecalis V583 suppressed the gelatinase activity and prevented biofilm formation. Sequence analysis indicated the presence of IS256 integrated into the fsrC gene at nucleotide position 321. Interestingly, IS256 is also associated with biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus. This is the first description of an insertion sequence that prevents biofilm formation in E. faecalis. Copyright de NRC Research PressPeer ReviewedCanadian Science PublishingConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2017201720152017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/143039reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1139/cjm-2015-0090Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1430392026-05-22T06:33:51Z
dc.title.none.fl_str_mv Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
title Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
spellingShingle Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
Pérez, Marta M.
Biofilms
Gelatinase
Insertion sequences
Enterococcus faecalis
title_short Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
title_full Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
title_fullStr Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
title_full_unstemmed Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
title_sort Is256 abolishes gelatinase activity and biofilm formation in a mutant of the nosocomial pathogen enterococcus faecalis v583
dc.creator.none.fl_str_mv Pérez, Marta M.
Calles-Enríquez, Marina
Río Lagar, Beatriz del
Ladero Losada, Víctor Manuel
Martín, M. Cruz
Fernández García, María
Álvarez González, Miguel Ángel
author Pérez, Marta M.
author_facet Pérez, Marta M.
Calles-Enríquez, Marina
Río Lagar, Beatriz del
Ladero Losada, Víctor Manuel
Martín, M. Cruz
Fernández García, María
Álvarez González, Miguel Ángel
author_role author
author2 Calles-Enríquez, Marina
Río Lagar, Beatriz del
Ladero Losada, Víctor Manuel
Martín, M. Cruz
Fernández García, María
Álvarez González, Miguel Ángel
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Biofilms
Gelatinase
Insertion sequences
Enterococcus faecalis
topic Biofilms
Gelatinase
Insertion sequences
Enterococcus faecalis
description Enterococcus faecalis is one of the most controversial species of lactic acid bacteria. Some strains are used as probiotics, while others are associated with severe and life-threatening nosocomial infections. Their pathogenicity depends on the acqui-sition of multidrug resistance and virulence factors. Gelatinase, which is required in the first steps of biofilm formation, is an important virulence determinant involved in E. faecalis pathogenesis, including endocarditis and peritonitis. The gene that codes for gelatinase (gelE) is controlled by the Fsr quorum-sensing system, whose encoding genes (fsrA, fsrB, fsrC, and fsrD) are located immediately upstream of gelE. The integration of a DNA fragment into the fsr locus of a derived mutant of E. faecalis V583 suppressed the gelatinase activity and prevented biofilm formation. Sequence analysis indicated the presence of IS256 integrated into the fsrC gene at nucleotide position 321. Interestingly, IS256 is also associated with biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus. This is the first description of an insertion sequence that prevents biofilm formation in E. faecalis. Copyright de NRC Research Press
publishDate 2015
dc.date.none.fl_str_mv 2015
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/143039
url http://hdl.handle.net/10261/143039
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1139/cjm-2015-0090
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Canadian Science Publishing
publisher.none.fl_str_mv Canadian Science Publishing
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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