Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules

18 p.-5 fig. We dedicate this work to the memory of our friend, mentor, and colleague, Jorge B. Schvartzman.

Detalhes bibliográficos
Autores: Cebrián, Jorge, Martínez, Víctor, Hernandez, Pablo, Krimer, Dora B., Martínez-Robles, María Luisa, Schvartzman, Jorge Bernardo, Fernández-Nestosa, María José
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/357772
Acesso em linha:http://hdl.handle.net/10261/357772
Access Level:Acceso aberto
Palavra-chave:DNA topology
2D gels
Supercoiling
Catenation
Knots
Topoisomerase
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spelling Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA moleculesCebrián, JorgeMartínez, VíctorHernandez, PabloKrimer, Dora B.Martínez-Robles, María LuisaSchvartzman, Jorge BernardoFernández-Nestosa, María JoséDNA topology2D gelsSupercoilingCatenationKnotsTopoisomerase18 p.-5 fig. We dedicate this work to the memory of our friend, mentor, and colleague, Jorge B. Schvartzman.Two-dimensional (2D) agarose gel electrophoresis is the method of choice to analyze DNA topology. The possibility to use E. coli strains with different genetic backgrounds in combination with nicking enzymes and different concentrations of norfloxacin improves the resolution of 2D gels to study the electrophoretic behavior of three different families of DNA topoisomers: supercoiled DNA molecules, post-replicative catenanes, and knotted DNA molecules. Here, we describe the materials and procedures required to optimize their separation by 2D gels. Understanding the differences in their electrophoretic behavior can help explain some important physical characteristics of these different types of DNA topoisomers.This work was sustained by grant BFU2011- 22489 to J.B.S. from the Spanish Ministerio de Economía y Competitividad. MJFN and VM were partially supported by an award granted by the National Council of Science and Technology (CONACYT), as Active Researchers of the National Incentive Program for Researchers (PRONII).Peer reviewedBio-protocolMinisterio de Economía y Competitividad (España)Consejo Nacional de Ciencia y Tecnología (Paraguay)Cebrián, Jorge [0000-0001-5070-0070]Hernandez, Pablo [0000-0002-8113-1277]Krimer, Dora B. [0000-0001-9972-3994]Schvartzman, Jorge Bernardo [0000-0002-1856-6278]Fernández-Nestosa, María José [0000-0001-7764-7791]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/357772reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN//BFU2011-22489https://doi.org/10.21769/BioProtoc.4983Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3577722026-05-22T06:33:51Z
dc.title.none.fl_str_mv Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
spellingShingle Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
Cebrián, Jorge
DNA topology
2D gels
Supercoiling
Catenation
Knots
Topoisomerase
title_short Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_full Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_fullStr Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_full_unstemmed Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
title_sort Electrophoretic mobility assay to separate supercoiled, catenated, and knotted DNA molecules
dc.creator.none.fl_str_mv Cebrián, Jorge
Martínez, Víctor
Hernandez, Pablo
Krimer, Dora B.
Martínez-Robles, María Luisa
Schvartzman, Jorge Bernardo
Fernández-Nestosa, María José
author Cebrián, Jorge
author_facet Cebrián, Jorge
Martínez, Víctor
Hernandez, Pablo
Krimer, Dora B.
Martínez-Robles, María Luisa
Schvartzman, Jorge Bernardo
Fernández-Nestosa, María José
author_role author
author2 Martínez, Víctor
Hernandez, Pablo
Krimer, Dora B.
Martínez-Robles, María Luisa
Schvartzman, Jorge Bernardo
Fernández-Nestosa, María José
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Consejo Nacional de Ciencia y Tecnología (Paraguay)
Cebrián, Jorge [0000-0001-5070-0070]
Hernandez, Pablo [0000-0002-8113-1277]
Krimer, Dora B. [0000-0001-9972-3994]
Schvartzman, Jorge Bernardo [0000-0002-1856-6278]
Fernández-Nestosa, María José [0000-0001-7764-7791]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv DNA topology
2D gels
Supercoiling
Catenation
Knots
Topoisomerase
topic DNA topology
2D gels
Supercoiling
Catenation
Knots
Topoisomerase
description 18 p.-5 fig. We dedicate this work to the memory of our friend, mentor, and colleague, Jorge B. Schvartzman.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/357772
url http://hdl.handle.net/10261/357772
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN//BFU2011-22489
https://doi.org/10.21769/BioProtoc.4983
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Bio-protocol
publisher.none.fl_str_mv Bio-protocol
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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