Study of methanogen communities associated with different rumen protozoal populations

Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were...

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Detalles Bibliográficos
Autores: Belanche, A., de la Fuente Oliver, Gabriel, Newbold, C. Jamie
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/49223
Acceso en línea:https://doi.org/10.1111/1574-6941.12423
http://hdl.handle.net/10459.1/49223
Access Level:acceso abierto
Palabra clave:archaea
endosymbiotic
holotrich
methanogens
rumen protozoa
Remugants
Protozous
Ruminants
Protozoa
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spelling Study of methanogen communities associated with different rumen protozoal populationsBelanche, A.de la Fuente Oliver, GabrielNewbold, C. Jamiearchaeaendosymbioticholotrichmethanogensrumen protozoaRemugantsProtozousRuminantsProtozoaProtozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7<br>3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67% similarity within treatment) in the rumen with similar diversity but different structures than PAM (P< 0.05). On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P< 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.This work was supported by the Commission of the European Communities (REDNEX project FP7-KBBE-2007-1) and the Welsh Government.Oxford University Press2015201520152015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://doi.org/10.1111/1574-6941.12423http://hdl.handle.net/10459.1/49223http://hdl.handle.net/10459.1/49223reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1111/1574-6941.12423Fems Microbiology Ecology, 2015, vol. 90, núm. 3, p. 663-677info:eu-repo/grantAgreement/EC/FP7/211606cc-by-nc, (c) Belanche, A. et al., 2015info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by-nc/4.0oai:recercat.cat:10459.1/492232026-05-29T05:05:01Z
dc.title.none.fl_str_mv Study of methanogen communities associated with different rumen protozoal populations
title Study of methanogen communities associated with different rumen protozoal populations
spellingShingle Study of methanogen communities associated with different rumen protozoal populations
Belanche, A.
archaea
endosymbiotic
holotrich
methanogens
rumen protozoa
Remugants
Protozous
Ruminants
Protozoa
title_short Study of methanogen communities associated with different rumen protozoal populations
title_full Study of methanogen communities associated with different rumen protozoal populations
title_fullStr Study of methanogen communities associated with different rumen protozoal populations
title_full_unstemmed Study of methanogen communities associated with different rumen protozoal populations
title_sort Study of methanogen communities associated with different rumen protozoal populations
dc.creator.none.fl_str_mv Belanche, A.
de la Fuente Oliver, Gabriel
Newbold, C. Jamie
author Belanche, A.
author_facet Belanche, A.
de la Fuente Oliver, Gabriel
Newbold, C. Jamie
author_role author
author2 de la Fuente Oliver, Gabriel
Newbold, C. Jamie
author2_role author
author
dc.subject.none.fl_str_mv archaea
endosymbiotic
holotrich
methanogens
rumen protozoa
Remugants
Protozous
Ruminants
Protozoa
topic archaea
endosymbiotic
holotrich
methanogens
rumen protozoa
Remugants
Protozous
Ruminants
Protozoa
description Protozoa-associated methanogens (PAM) are considered one of the most active communities in the rumen methanogenesis. This experiment investigated whether methanogens are sequestrated within rumen protozoa, and structural differences between rumen free-living methanogens and PAM. Rumen protozoa were harvested from totally faunated sheep, and six protozoal fractions (plus free-living microorganisms) were generated by sequential filtration. Holotrich-monofaunated sheep were also used to investigate the holotrich-associated methanogens. Protozoal size determined the number of PAM as big protozoa had 1.7<br>3.3 times more methanogen DNA than smaller protozoa, but also more endosymbiotic bacteria (2.2- to 3.5-fold times). Thus, similar abundance of methanogens with respect to total bacteria were observed across all protozoal fractions and free-living microorganisms, suggesting that methanogens are not accumulated within rumen protozoa in a greater proportion to that observed in the rumen as a whole. All rumen methanogen communities had similar diversity (22.2 ± 3.4 TRFs). Free-living methanogens composed a conserved community (67% similarity within treatment) in the rumen with similar diversity but different structures than PAM (P< 0.05). On the contrary, PAM constituted a more variable community (48% similarity), which differed between holotrich and total protozoa (P< 0.001). Thus, PAM constitutes a community, which requires further investigation as part of methane mitigation strategies.
publishDate 2015
dc.date.none.fl_str_mv 2015
2015
2015
2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.1111/1574-6941.12423
http://hdl.handle.net/10459.1/49223
http://hdl.handle.net/10459.1/49223
url https://doi.org/10.1111/1574-6941.12423
http://hdl.handle.net/10459.1/49223
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1111/1574-6941.12423
Fems Microbiology Ecology, 2015, vol. 90, núm. 3, p. 663-677
info:eu-repo/grantAgreement/EC/FP7/211606
dc.rights.none.fl_str_mv cc-by-nc, (c) Belanche, A. et al., 2015
info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by-nc/4.0
rights_invalid_str_mv cc-by-nc, (c) Belanche, A. et al., 2015
http://creativecommons.org/licenses/by-nc/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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