Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography

The high proportion of acetonitrile used in many HILIC mobile phases significantly changes the acid-base properties of pH buffers and analytes foreseen from available data in water. In this paper, the recommended stability pH range for chromatographic columns is examined with various acetonitrile wa...

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Autores: Alvarez-Segura, Támara, Subirats i Vila, Xavier, Rosés Pascual, Martí
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2018
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/127710
Acceso en línea:https://hdl.handle.net/2445/127710
Access Level:acceso abierto
Palabra clave:Cromatografia de líquids
Equilibri àcid-base
Concentració dels ions d'hidrogen
Liquid chromatography
Acid-base equilibrium
Hydrogen-ion concentration
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spelling Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatographyAlvarez-Segura, TámaraSubirats i Vila, XavierRosés Pascual, MartíCromatografia de líquidsEquilibri àcid-baseConcentració dels ions d'hidrogenLiquid chromatographyAcid-base equilibriumHydrogen-ion concentrationThe high proportion of acetonitrile used in many HILIC mobile phases significantly changes the acid-base properties of pH buffers and analytes foreseen from available data in water. In this paper, the recommended stability pH range for chromatographic columns is examined with various acetonitrile water mixtures, resulting in a significant broadening in the operational pH window with the content of organic solvent. Additionally, the challenge of buffer selection in HILIC is also addressed. Commonly used ammonium acetate shrinks its pH buffering range in acetonitrile-rich mobile phases due to variations in the dissociation constants of the buffer constituents (acetic acid and ammonium). Thus, other organic acids such as formic acid, TFA, and succinimide have been studied as buffers in order to fully cover the pH range of use of the column. Also the retention-pH profiles of several acids and bases have been studied in 80% and 90% acetonitrile using the proposed buffers and their behavior compared to that obtained with buffers prepared from oxalic acid, pyrrolidine, and triethylamine. The latter two show additional interactions in 80% acetonitrile that distort the expected retention-pH profiles of acid analytes, but not the ones of bases. In 90% acetonitrile the profiles are affected by significant additional solute-buffer interactions that might be caused by ion pairing, homo- and heteroassociation in this low ion solvating medium.Elsevier B.V.2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/127710Articles publicats en revistes (Enginyeria Química i Química Analítica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.aca.2018.11.021Analytica Chimica Acta, 2018, vol. 1050, p. 176-184https://doi.org/10.1016/j.aca.2018.11.021cc-by-nc-nd (c) Elsevier B.V., 2018http://creativecommons.org/licenses/by-nc-nd/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1277102026-05-27T06:46:51Z
dc.title.none.fl_str_mv Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
title Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
spellingShingle Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
Alvarez-Segura, Támara
Cromatografia de líquids
Equilibri àcid-base
Concentració dels ions d'hidrogen
Liquid chromatography
Acid-base equilibrium
Hydrogen-ion concentration
title_short Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
title_full Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
title_fullStr Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
title_full_unstemmed Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
title_sort Retention-pH profiles of acids and bases in hydrophilic interaction liquid chromatography
dc.creator.none.fl_str_mv Alvarez-Segura, Támara
Subirats i Vila, Xavier
Rosés Pascual, Martí
author Alvarez-Segura, Támara
author_facet Alvarez-Segura, Támara
Subirats i Vila, Xavier
Rosés Pascual, Martí
author_role author
author2 Subirats i Vila, Xavier
Rosés Pascual, Martí
author2_role author
author
dc.subject.none.fl_str_mv Cromatografia de líquids
Equilibri àcid-base
Concentració dels ions d'hidrogen
Liquid chromatography
Acid-base equilibrium
Hydrogen-ion concentration
topic Cromatografia de líquids
Equilibri àcid-base
Concentració dels ions d'hidrogen
Liquid chromatography
Acid-base equilibrium
Hydrogen-ion concentration
description The high proportion of acetonitrile used in many HILIC mobile phases significantly changes the acid-base properties of pH buffers and analytes foreseen from available data in water. In this paper, the recommended stability pH range for chromatographic columns is examined with various acetonitrile water mixtures, resulting in a significant broadening in the operational pH window with the content of organic solvent. Additionally, the challenge of buffer selection in HILIC is also addressed. Commonly used ammonium acetate shrinks its pH buffering range in acetonitrile-rich mobile phases due to variations in the dissociation constants of the buffer constituents (acetic acid and ammonium). Thus, other organic acids such as formic acid, TFA, and succinimide have been studied as buffers in order to fully cover the pH range of use of the column. Also the retention-pH profiles of several acids and bases have been studied in 80% and 90% acetonitrile using the proposed buffers and their behavior compared to that obtained with buffers prepared from oxalic acid, pyrrolidine, and triethylamine. The latter two show additional interactions in 80% acetonitrile that distort the expected retention-pH profiles of acid analytes, but not the ones of bases. In 90% acetonitrile the profiles are affected by significant additional solute-buffer interactions that might be caused by ion pairing, homo- and heteroassociation in this low ion solvating medium.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/127710
url https://hdl.handle.net/2445/127710
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.aca.2018.11.021
Analytica Chimica Acta, 2018, vol. 1050, p. 176-184
https://doi.org/10.1016/j.aca.2018.11.021
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2018
http://creativecommons.org/licenses/by-nc-nd/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Enginyeria Química i Química Analítica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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