Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes

Pterostilbene is a dimethyl ether derivative of resveratrol, less metabolized than its analogue, due to the substitution of two hydroxyl groups with methoxyl groups. Nevertheless, the amounts of pterostilbene phase II metabolites found in plasma and tissues are higher than those of the parent compou...

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Autores: Trepiana Arin, Jenifer, Krisa, Stéphanie, Portillo Baquedano, María Puy
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/49021
Acceso en línea:http://hdl.handle.net/10810/49021
Access Level:acceso abierto
Palabra clave:pterostilbene metabolites
pterostilbene-4′-O-glucuronide
pterostilbene-4′-O-sulfate
liver steatosis
AML-12 hepatocytes
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spelling Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured HepatocytesTrepiana Arin, JeniferKrisa, StéphaniePortillo Baquedano, María Puypterostilbene metabolitespterostilbene-4′-O-glucuronidepterostilbene-4′-O-sulfateliver steatosisAML-12 hepatocytesPterostilbene is a dimethyl ether derivative of resveratrol, less metabolized than its analogue, due to the substitution of two hydroxyl groups with methoxyl groups. Nevertheless, the amounts of pterostilbene phase II metabolites found in plasma and tissues are higher than those of the parent compound. The first aim of this study was to assess whether pterostilbene-4′-O-glucuronide (PT-G) and pterostilbene-4′-O-sulfate (PT-S) were able to prevent triglyceride accumulation in AML12 (alpha mouse liver 12) hepatocytes. This being the case, we aimed to analyze the mechanisms involved in their effects. For this purpose, an in vitro model mimicking the hepatocyte situation in fatty liver was developed by incubating mouse AML12 hepatocytes with palmitic acid (PA). For cell treatments, hepatocytes were incubated with 1, 10 or 25 µM of pterostilbene, pterostilbene-4′-O-glucuronide or pterostilbene-4′-O-sulfate for 18 h. Triglycerides and cell viability were assessed by a commercial kit and crystal violet assay, respectively. Protein expression of enzymes and transporters involved in triglyceride metabolism was analyzed by immunoblot. The results showed for the first time the anti-steatotic effect of pterostilbene metabolites and thus, that they contribute to the preventive effect induced by pterostilbene on steatosis in in vivo models. This anti-steatotic effect is mainly due to the inhibition of de novo lipogenesis.This research was funded by Ministerio de Economía y Competitividad-Fondo Europeo de Desarrollo Regional (grant number AGL-2015-65719-R MINECO/FEDER, UE), Instituto de Salud Carlos III CIBERobn (grant number CB12/03/30007) and University of the Basque Country (grant number GIU 18/173).MDPI2020202020202020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/49021reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/AGL-2015-65719-R/https://www.mdpi.com/1420-3049/25/22/5444/htminfo:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/490212026-06-18T09:23:17Z
dc.title.none.fl_str_mv Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
title Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
spellingShingle Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
Trepiana Arin, Jenifer
pterostilbene metabolites
pterostilbene-4′-O-glucuronide
pterostilbene-4′-O-sulfate
liver steatosis
AML-12 hepatocytes
title_short Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
title_full Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
title_fullStr Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
title_full_unstemmed Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
title_sort Activity of Pterostilbene Metabolites against Liver Steatosis in Cultured Hepatocytes
dc.creator.none.fl_str_mv Trepiana Arin, Jenifer
Krisa, Stéphanie
Portillo Baquedano, María Puy
author Trepiana Arin, Jenifer
author_facet Trepiana Arin, Jenifer
Krisa, Stéphanie
Portillo Baquedano, María Puy
author_role author
author2 Krisa, Stéphanie
Portillo Baquedano, María Puy
author2_role author
author
dc.subject.none.fl_str_mv pterostilbene metabolites
pterostilbene-4′-O-glucuronide
pterostilbene-4′-O-sulfate
liver steatosis
AML-12 hepatocytes
topic pterostilbene metabolites
pterostilbene-4′-O-glucuronide
pterostilbene-4′-O-sulfate
liver steatosis
AML-12 hepatocytes
description Pterostilbene is a dimethyl ether derivative of resveratrol, less metabolized than its analogue, due to the substitution of two hydroxyl groups with methoxyl groups. Nevertheless, the amounts of pterostilbene phase II metabolites found in plasma and tissues are higher than those of the parent compound. The first aim of this study was to assess whether pterostilbene-4′-O-glucuronide (PT-G) and pterostilbene-4′-O-sulfate (PT-S) were able to prevent triglyceride accumulation in AML12 (alpha mouse liver 12) hepatocytes. This being the case, we aimed to analyze the mechanisms involved in their effects. For this purpose, an in vitro model mimicking the hepatocyte situation in fatty liver was developed by incubating mouse AML12 hepatocytes with palmitic acid (PA). For cell treatments, hepatocytes were incubated with 1, 10 or 25 µM of pterostilbene, pterostilbene-4′-O-glucuronide or pterostilbene-4′-O-sulfate for 18 h. Triglycerides and cell viability were assessed by a commercial kit and crystal violet assay, respectively. Protein expression of enzymes and transporters involved in triglyceride metabolism was analyzed by immunoblot. The results showed for the first time the anti-steatotic effect of pterostilbene metabolites and thus, that they contribute to the preventive effect induced by pterostilbene on steatosis in in vivo models. This anti-steatotic effect is mainly due to the inhibition of de novo lipogenesis.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/49021
url http://hdl.handle.net/10810/49021
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/AGL-2015-65719-R/
https://www.mdpi.com/1420-3049/25/22/5444/htm
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
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