Protocol for stimulating specific rodent limb receptive fields while recording in vivo somatosensory-evoked activity

Studies on sensory information processing typically focus on whisker-related tactile information, overlooking the question of how sensory inputs from other body areas are processed at cortical levels. Here, we present a protocol for stimulating specific rodent limb receptive fields while recording i...

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Detalles Bibliográficos
Autor: Aguilar Lepe, Juan de los Reyes
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universidad de Castilla-La Mancha
Repositorio:RUIdeRA. Repositorio Institucional de la UCLM
OAI Identifier:oai:ruidera.uclm.es:10578/42352
Acceso en línea:http://10.1016/j.xpro.2024.102972
https://hdl.handle.net/10578/42352
Access Level:acceso abierto
Palabra clave:Behavior
Health Sciences
In vivo
Limb
Neuroscience
Receptive fields
Rodent
Somatosensory
Descripción
Sumario:Studies on sensory information processing typically focus on whisker-related tactile information, overlooking the question of how sensory inputs from other body areas are processed at cortical levels. Here, we present a protocol for stimulating specific rodent limb receptive fields while recording in vivo somatosensory-evoked activity. We describe steps for localizing cortical-hindlimb coordinates using acute peripheral stimulation, electrode placement, and the application of electrical stimulation. This protocol overcomes the challenge of inducing a reproducible and consistent stimulation of specific limbs.