Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation

Muscle cell differentiation caused a reduction of glucose transport, GLUT1 glucose transporter expression, and GLUT1 mRNA levels. A fragment of 2.1 kilobases of the rat GLUT1 gene linked to chloramphenicol acetyltransferase drove transcriptional activity in myoblasts, and differentiation caused a de...

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Autores: Viñals Canals, Francesc, Fandos Espallargas, César, Santalucía Albi, Tomàs, Ferré, Josep, Testar, Xavier, Palacín Prieto, Manuel, Zorzano Olarte, Antonio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:1997
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177637
Acceso en línea:https://hdl.handle.net/2445/177637
Access Level:acceso abierto
Palabra clave:Expressió gènica
Monosacàrids
Músculs
Gene expression
Monosaccharides
Muscles
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repository_id_str
spelling Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiationViñals Canals, FrancescFandos Espallargas, CésarSantalucía Albi, TomàsFerré, JosepTestar, XavierPalacín Prieto, ManuelZorzano Olarte, AntonioExpressió gènicaMonosacàridsMúsculsGene expressionMonosaccharidesMusclesMuscle cell differentiation caused a reduction of glucose transport, GLUT1 glucose transporter expression, and GLUT1 mRNA levels. A fragment of 2.1 kilobases of the rat GLUT1 gene linked to chloramphenicol acetyltransferase drove transcriptional activity in myoblasts, and differentiation caused a decrease in transcription. Transient transfection of 5' and 3' deletion constructs showed that the fragment -99/-33 of the GLUT1 gene drives transcriptional activity of the GLUT1 gene and participates in the reduced transcription after muscle differentiation. Electrophoretic mobility shift assays showed the binding of Sp1 protein to the fragment -102/-37 in the myoblast state but not in myotubes, and Sp1 was found to transactivate the GLUT1 promoter. Western blot analysis indicated that Sp1 was drastically down-regulated during myogenesis. Furthermore, the forced over-expression of MyoD in C3H10T1/2 cells mimicked the effects observed during myogenesis, Sp1 down-regulation and reduced transcriptional activity of the GLUT1 gene promoter. In all, these data suggest a regulatory model in which MyoD activation during myogenesis causes the down-regulation of Sp1, which contributes to the repression of GLUT1 gene transcription and, therefore, leads to the reduction in GLUT1 expression and glucose transport.American Society for Biochemistry and Molecular Biology1997info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177637Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1074/jbc.272.20.12913Journal of Biological Chemistry, 1997, vol. 272, num. 20, p. 12913-12921https://doi.org/10.1074/jbc.272.20.12913(c) American Society for Biochemistry and Molecular Biology, 1997info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1776372026-05-27T06:46:51Z
dc.title.none.fl_str_mv Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
title Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
spellingShingle Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
Viñals Canals, Francesc
Expressió gènica
Monosacàrids
Músculs
Gene expression
Monosaccharides
Muscles
title_short Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
title_full Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
title_fullStr Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
title_full_unstemmed Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
title_sort Myogenesis and MyoD down-regulate Sp1. A mechanism for the repression of GLUT1 during muscle cell differentiation
dc.creator.none.fl_str_mv Viñals Canals, Francesc
Fandos Espallargas, César
Santalucía Albi, Tomàs
Ferré, Josep
Testar, Xavier
Palacín Prieto, Manuel
Zorzano Olarte, Antonio
author Viñals Canals, Francesc
author_facet Viñals Canals, Francesc
Fandos Espallargas, César
Santalucía Albi, Tomàs
Ferré, Josep
Testar, Xavier
Palacín Prieto, Manuel
Zorzano Olarte, Antonio
author_role author
author2 Fandos Espallargas, César
Santalucía Albi, Tomàs
Ferré, Josep
Testar, Xavier
Palacín Prieto, Manuel
Zorzano Olarte, Antonio
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv Expressió gènica
Monosacàrids
Músculs
Gene expression
Monosaccharides
Muscles
topic Expressió gènica
Monosacàrids
Músculs
Gene expression
Monosaccharides
Muscles
description Muscle cell differentiation caused a reduction of glucose transport, GLUT1 glucose transporter expression, and GLUT1 mRNA levels. A fragment of 2.1 kilobases of the rat GLUT1 gene linked to chloramphenicol acetyltransferase drove transcriptional activity in myoblasts, and differentiation caused a decrease in transcription. Transient transfection of 5' and 3' deletion constructs showed that the fragment -99/-33 of the GLUT1 gene drives transcriptional activity of the GLUT1 gene and participates in the reduced transcription after muscle differentiation. Electrophoretic mobility shift assays showed the binding of Sp1 protein to the fragment -102/-37 in the myoblast state but not in myotubes, and Sp1 was found to transactivate the GLUT1 promoter. Western blot analysis indicated that Sp1 was drastically down-regulated during myogenesis. Furthermore, the forced over-expression of MyoD in C3H10T1/2 cells mimicked the effects observed during myogenesis, Sp1 down-regulation and reduced transcriptional activity of the GLUT1 gene promoter. In all, these data suggest a regulatory model in which MyoD activation during myogenesis causes the down-regulation of Sp1, which contributes to the repression of GLUT1 gene transcription and, therefore, leads to the reduction in GLUT1 expression and glucose transport.
publishDate 1997
dc.date.none.fl_str_mv 1997
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177637
url https://hdl.handle.net/2445/177637
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1074/jbc.272.20.12913
Journal of Biological Chemistry, 1997, vol. 272, num. 20, p. 12913-12921
https://doi.org/10.1074/jbc.272.20.12913
dc.rights.none.fl_str_mv (c) American Society for Biochemistry and Molecular Biology, 1997
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) American Society for Biochemistry and Molecular Biology, 1997
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
publisher.none.fl_str_mv American Society for Biochemistry and Molecular Biology
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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