Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes

A direct competitive immunosensor for the electrochemical determination of Imidacloprid (IMD) pesticide on gold nanoparticle-modified screen-printed carbon electrodes (AuNP-SPCE) is here reported for the first time. Self-obtained specific monoclonal antibodies are immobilized on the AuNP-SPCE taking...

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Autores: Pérez-Fernández, Beatriz, Mercader Badia, Josep Vicent, Abad Fuentes, Antonio, Checa Orrego, Brenda I., Costa-García, Agustín, de la Escosura Muñiz, Alfredo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/201723
Acceso en línea:http://hdl.handle.net/10261/201723
Access Level:acceso abierto
Palabra clave:Electrochemical immunosensor
Screen-printed carbon electrodes
Imidacloprid
Point-of-use
Pesticides
Nanoparticles
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spelling Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodesPérez-Fernández, BeatrizMercader Badia, Josep VicentAbad Fuentes, AntonioCheca Orrego, Brenda I.Costa-García, Agustínde la Escosura Muñiz, AlfredoElectrochemical immunosensorScreen-printed carbon electrodesImidaclopridPoint-of-usePesticidesNanoparticlesA direct competitive immunosensor for the electrochemical determination of Imidacloprid (IMD) pesticide on gold nanoparticle-modified screen-printed carbon electrodes (AuNP-SPCE) is here reported for the first time. Self-obtained specific monoclonal antibodies are immobilized on the AuNP-SPCE taking advantage of the AuNPs biofunctionalization abilities. In our biosensor design, free IMD in the sample competes with IMD conjugated with horseradish peroxidase (IMD-HRP) for the recognition by the antibodies. After that, 3,3′,5,5′-Tetramethylbenzidine (TMB) is enzymatically oxidized by HRP, followed by the oxidized TMB reduction back at the surface of the SPCE. This process gives an associated catalytic current (analytical signal) that is inversely proportional to the IMD amount. The main parameters affecting the analytical signal have been optimized, reaching a good precision (repeatability with a RSD of 6%), accuracy (relative error of 6%), stability (up to one month), selectivity and an excellent limit of detection (LOD of 22 pmol L−1), below the maximum levels allowed by the legislation, with a wide response range (50–10000 pmol L−1). The detection through antibodies also allows to have an excellent selectivity against other pesticides potentially present in real samples. Low matrix effects were found when analysing IMD in tap water and watermelon samples. The electrochemical immunosensor was also validated with HPLC-MS/MS, the reference method used in official laboratories for IMD analysis, through statistical tests. Our findings make the electrochemical immunosensor as an outstanding method for the rapid and sensitive determination of IMD at the point-of-use.This work has been supported by the FC-GRUPIN-ID/2018/000166 project from the Asturias Regional Government and the CTQ2017-86994-R project from the Spanish Ministry Economy and Competitiveness (MINECO). A. de la Escosura-Muñiz acknowledges the Spanish Ministry of Science, Innovation and Universities (MICINN) for the “Ramón y Cajal” Research Fellow (RyC-2016-20299).Peer reviewedElsevierMinisterio de Economía y Competitividad (España)Principado de AsturiasMinisterio de Ciencia, Innovación y Universidades (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/201723reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-86994-Rhttps://doi.org/10.1016/j.talanta.2019.120465Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2017232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
title Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
spellingShingle Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
Pérez-Fernández, Beatriz
Electrochemical immunosensor
Screen-printed carbon electrodes
Imidacloprid
Point-of-use
Pesticides
Nanoparticles
title_short Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
title_full Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
title_fullStr Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
title_full_unstemmed Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
title_sort Direct competitive immunosensor for Imidacloprid pesticide detection on gold nanoparticle-modified electrodes
dc.creator.none.fl_str_mv Pérez-Fernández, Beatriz
Mercader Badia, Josep Vicent
Abad Fuentes, Antonio
Checa Orrego, Brenda I.
Costa-García, Agustín
de la Escosura Muñiz, Alfredo
author Pérez-Fernández, Beatriz
author_facet Pérez-Fernández, Beatriz
Mercader Badia, Josep Vicent
Abad Fuentes, Antonio
Checa Orrego, Brenda I.
Costa-García, Agustín
de la Escosura Muñiz, Alfredo
author_role author
author2 Mercader Badia, Josep Vicent
Abad Fuentes, Antonio
Checa Orrego, Brenda I.
Costa-García, Agustín
de la Escosura Muñiz, Alfredo
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Principado de Asturias
Ministerio de Ciencia, Innovación y Universidades (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Electrochemical immunosensor
Screen-printed carbon electrodes
Imidacloprid
Point-of-use
Pesticides
Nanoparticles
topic Electrochemical immunosensor
Screen-printed carbon electrodes
Imidacloprid
Point-of-use
Pesticides
Nanoparticles
description A direct competitive immunosensor for the electrochemical determination of Imidacloprid (IMD) pesticide on gold nanoparticle-modified screen-printed carbon electrodes (AuNP-SPCE) is here reported for the first time. Self-obtained specific monoclonal antibodies are immobilized on the AuNP-SPCE taking advantage of the AuNPs biofunctionalization abilities. In our biosensor design, free IMD in the sample competes with IMD conjugated with horseradish peroxidase (IMD-HRP) for the recognition by the antibodies. After that, 3,3′,5,5′-Tetramethylbenzidine (TMB) is enzymatically oxidized by HRP, followed by the oxidized TMB reduction back at the surface of the SPCE. This process gives an associated catalytic current (analytical signal) that is inversely proportional to the IMD amount. The main parameters affecting the analytical signal have been optimized, reaching a good precision (repeatability with a RSD of 6%), accuracy (relative error of 6%), stability (up to one month), selectivity and an excellent limit of detection (LOD of 22 pmol L−1), below the maximum levels allowed by the legislation, with a wide response range (50–10000 pmol L−1). The detection through antibodies also allows to have an excellent selectivity against other pesticides potentially present in real samples. Low matrix effects were found when analysing IMD in tap water and watermelon samples. The electrochemical immunosensor was also validated with HPLC-MS/MS, the reference method used in official laboratories for IMD analysis, through statistical tests. Our findings make the electrochemical immunosensor as an outstanding method for the rapid and sensitive determination of IMD at the point-of-use.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/201723
url http://hdl.handle.net/10261/201723
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTQ2017-86994-R
https://doi.org/10.1016/j.talanta.2019.120465
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.81155

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