A successful method for phycocyanin extraction from Arthrospira platensisusing [Emim] [EtSO4] ionic liquid

Research into the extraction of phycobiliprotein from Arthrospira platensis is currently ongoing. Most traditional methods of phycobiliprotein extraction include the use of organic solvents, which negatively affect the sustainability of the process. Ionic liquids (ILs) are a promising alternative fo...

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Detalles Bibliográficos
Autores: Sánchez-Laso, Jennifer, Piera, Alejandro, Vicente, Gemma, Bautista, Luis Fernando, Rodríguez, Rosalía, Espada, Juan J.
Tipo de recurso: artículo
Fecha de publicación:2021
País:España
Institución:Universidad Rey Juan Carlos
Repositorio:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
OAI Identifier:oai:burjcdigital.urjc.es:10115/18608
Acceso en línea:http://hdl.handle.net/10115/18608
Access Level:acceso abierto
Palabra clave:Arthrospira platensis
bioproducts
phycobiliprotein
phycocyanin
ionic liquid
extraction
Descripción
Sumario:Research into the extraction of phycobiliprotein from Arthrospira platensis is currently ongoing. Most traditional methods of phycobiliprotein extraction include the use of organic solvents, which negatively affect the sustainability of the process. Ionic liquids (ILs) are a promising alternative for phycobiliprotein extraction due to their properties as green solvents. In the present work an imidazolium-based IL and sonication were studied for phycobiliprotein and carbohydrate extraction. A factorial experimental design was used to optimize the amount of extracted phycocyanin. The maximum extraction yield was achieved by using low biomass/solvent ratios combined with high IL/water ratios and sonication powers, and long operation times. The recovery of IL was studied under these conditions using a dialysis-based process to separate the IL from the extracted phycobiliproteins. The results reveal the possibility of using the recovered IL for seven consecutive extraction cycles with an acceptable phycocyanin extraction amount, from 75 mg g−1 (fresh IL) to 60 mg g−1 (7 cycles of reused IL). The use of [Emim] [EtSO4] + ultrasound is a promising alternative for phycocyanin extraction, enhancing the results obtained with other IL in terms of extracted phycocyanin and the number of times the IL can be reused.