Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research

Advances in regenerative medicine have enabled the search for new solutions to current health problems in so far unexplored fields. Thus, we focused on cadaveric subcutaneous fat as a promising source of adipose-derived stem cells (ADSCs) that have potential to differentiate into different cell line...

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Autores: Milián L, Molina P, Oliver-Ferrándiz M, Fernández-Sellers C, Monzó A, Sánchez-Sánchez R, Braza-Boils A, Mata M, Zorio E
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Institución:INCLIVA
Repositorio:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
OAI Identifier:oai:incliva.fundanetsuite.com:p17795
Acceso en línea:https://incliva.portalinvestigacion.com/publicaciones/17795
Access Level:acceso abierto
Palabra clave:adipose-derived stem cells
mesenchymal cell
cartilage regeneration
bone regeneration
tissue engineering
in vitro disease models
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spelling Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and ResearchMilián LMolina POliver-Ferrándiz MFernández-Sellers CMonzó ASánchez-Sánchez RBraza-Boils AMata MZorio Eadipose-derived stem cellsmesenchymal cellcartilage regenerationbone regenerationtissue engineeringin vitro disease modelsAdvances in regenerative medicine have enabled the search for new solutions to current health problems in so far unexplored fields. Thus, we focused on cadaveric subcutaneous fat as a promising source of adipose-derived stem cells (ADSCs) that have potential to differentiate into different cell lines. With this aim, we isolated and characterized ADSCs from cadaveric samples with a postmortem interval ranging from 30 to 55 h and evaluated their ability to differentiate into chondrocytes or osteocytes. A commercial ADSC line was used as reference. Morphological and protein expression analyses were used to confirm the final stage of differentiation. Eight out of fourteen samples from patients were suitable to complete the whole protocol. Cadaveric ADSCs exhibited features of stem cells based upon several markers: CD29 (84.49 +/- 14.07%), CD105 (94.38 +/- 2.09%), and CD44 (99.77 +/- 0.32%). The multiparametric assessment of differentiation confirmed the generation of stable lines of chondrocytes and osteocytes. In conclusion, we provide evidence supporting the feasibility of obtaining viable postmortem human subcutaneous fat ADSCs with potential application in tissue engineering and research fields.MDPI2023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://incliva.portalinvestigacion.com/publicaciones/17795INTERNATIONAL JOURNAL OF MOLECULAR SCIENCESISSN: 16616596ISSNe: 14220067reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVAinstname:INCLIVAInglésinfo:eu-repo/semantics/openAccessoai:incliva.fundanetsuite.com:p177952026-06-07T16:35:31Z
dc.title.none.fl_str_mv Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
title Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
spellingShingle Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
Milián L
adipose-derived stem cells
mesenchymal cell
cartilage regeneration
bone regeneration
tissue engineering
in vitro disease models
title_short Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
title_full Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
title_fullStr Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
title_full_unstemmed Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
title_sort Cadaveric Adipose-Derived Stem Cells for Regenerative Medicine and Research
dc.creator.none.fl_str_mv Milián L
Molina P
Oliver-Ferrándiz M
Fernández-Sellers C
Monzó A
Sánchez-Sánchez R
Braza-Boils A
Mata M
Zorio E
author Milián L
author_facet Milián L
Molina P
Oliver-Ferrándiz M
Fernández-Sellers C
Monzó A
Sánchez-Sánchez R
Braza-Boils A
Mata M
Zorio E
author_role author
author2 Molina P
Oliver-Ferrándiz M
Fernández-Sellers C
Monzó A
Sánchez-Sánchez R
Braza-Boils A
Mata M
Zorio E
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv adipose-derived stem cells
mesenchymal cell
cartilage regeneration
bone regeneration
tissue engineering
in vitro disease models
topic adipose-derived stem cells
mesenchymal cell
cartilage regeneration
bone regeneration
tissue engineering
in vitro disease models
description Advances in regenerative medicine have enabled the search for new solutions to current health problems in so far unexplored fields. Thus, we focused on cadaveric subcutaneous fat as a promising source of adipose-derived stem cells (ADSCs) that have potential to differentiate into different cell lines. With this aim, we isolated and characterized ADSCs from cadaveric samples with a postmortem interval ranging from 30 to 55 h and evaluated their ability to differentiate into chondrocytes or osteocytes. A commercial ADSC line was used as reference. Morphological and protein expression analyses were used to confirm the final stage of differentiation. Eight out of fourteen samples from patients were suitable to complete the whole protocol. Cadaveric ADSCs exhibited features of stem cells based upon several markers: CD29 (84.49 +/- 14.07%), CD105 (94.38 +/- 2.09%), and CD44 (99.77 +/- 0.32%). The multiparametric assessment of differentiation confirmed the generation of stable lines of chondrocytes and osteocytes. In conclusion, we provide evidence supporting the feasibility of obtaining viable postmortem human subcutaneous fat ADSCs with potential application in tissue engineering and research fields.
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://incliva.portalinvestigacion.com/publicaciones/17795
url https://incliva.portalinvestigacion.com/publicaciones/17795
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
ISSN: 16616596
ISSNe: 14220067
reponame:r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
instname:INCLIVA
instname_str INCLIVA
reponame_str r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
collection r-INCLIVA. Repositorio Institucional de Producción Científica de INCLIVA
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,81155

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