A Preclinical Model to Assess Intestinal Barrier Integrity Using Canine Enteroids and Colonoids

While two-dimensional (2D) cell cultures, such as Caco-2 and Madin–Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these two-dimensional in vitro systems present inherent limitations in replicating the complexities of in vivo biology. Recent progress in three-dime...

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Detalles Bibliográficos
Autores: Corbett, Megan P., Gabriel, Vojtech, Livania, Vanessa, Díaz-Regañón Fernández, David Rafael, Ralston, Abigail, Zdyrski, Christopher, Liu, Dongjie, Minkler, Sarah, Wickham, Hannah, Lincoln, Addison, Paukner, Karel, Atherly, Todd, Merodio, María M., Sahoo, Dipak Kumar, Meyerholz, David K., Allenspach, Karin, Mochel, Jonathan P.
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/119276
Acceso en línea:https://hdl.handle.net/20.500.14352/119276
Access Level:acceso abierto
Palabra clave:636.09
Transwell
Canine
Organoid
Caco-2
TEM
MDCK
TEER
Veterinaria
3109 Ciencias Veterinarias
Descripción
Sumario:While two-dimensional (2D) cell cultures, such as Caco-2 and Madin–Darby canine kidney (MDCK) cells are widely used in a variety of biological models, these two-dimensional in vitro systems present inherent limitations in replicating the complexities of in vivo biology. Recent progress in three-dimensional organoid technology has the potential to address these limitations. In this study, the characteristics of conventional 2D cell culture systems were compared to those of canine intestinal organoids (enteroids, ENT, and colonoids, COL). Light microscopy and transmission electron microscopy were employed to evaluate the microanatomy of ENT, COL, Caco-2, and MDCK cell monolayers, while transepithelial electrical resistance (TEER) values were measured to assess monolayer integrity. The TEER values of canine ENT monolayers more closely approximated reported TEER values for human small intestines compared to Caco-2 and MDCK monolayers. Additionally, canine ENT demonstrated greater monolayer stability than Caco-2 and MDCK cells. Notably, while all systems displayed desmosomes, canine ENT and COL exclusively produced mucus. These findings highlight the potential of the canine organoid system as a more biologically relevant model for in vitro studies, addressing the limitations of conventional 2D cell culture systems.

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