An engineered periosteum for efficient delivery of rhBMP-2 and mesenchymal progenitor cells during bone regeneration

During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (et...

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Detalhes bibliográficos
Autores: Romero-Torrecilla, J.A. (Juan Antonio)|||/items/0d94fc20-5c0d-42aa-8da5-e162ef65978d, Lamo-de-Espinosa-Vázquez-de-Sola, J.M. (José María)|||/items/75feb837-6d53-41fe-808d-4b70661db44e, Ripalda-Cemboráin, P. (Purificación)|||/items/844f1426-2ea0-484d-954c-08f0246aca72, López-Martínez, T. (Tania)|||/items/93d44011-8bb7-44e8-9431-66fe447c5d35, Abizanda-Sarasa, G. (Gloria)|||/items/1e76a0e3-32d0-424e-b58a-d9ddf7a82ef7, Riera-Alvarez, L. (Luis)|||/items/58239e38-f31c-4d1c-b1d3-de4e40e9f4b8, Ruiz-de-Galarreta-Moriones, S.(Sergio)|||/items/3320edac-66b8-4179-b4c1-ded047961ad3, López-Barberena, A. (Asier)|||/items/a9c76e03-b2d5-493c-b6f8-ad37626d2d98, Rodríguez-Florez, N. (Naiara)|||/items/0e6cedfb-6a96-4e19-b698-c97e11c6d94c, Elizalde, R. (Reyes)|||/items/7d5ff705-4327-4fe5-a4c9-7da53b4da3aa, Jayawarna, V. (Vineetha)|||/items/58e6724b-2cf0-4125-b322-66c34aa5d328, Valdés-Fernández, J. (José)|||/items/944a0294-b6a1-4ebb-823b-7715d38a1dba, Echanove-González De Anleo, M. (Miguel)|||/items/04dbf34a-5f19-4c4a-b4d3-f02e0f6e9d8c, Childs, P. (Peter)|||/items/4ea4f21b-7fcb-4202-b65c-e50872331486, Juan-Pardo, E.M. (Elena M.) de|||/items/9502b2ed-6f70-4303-be76-728f46cc1342, Salmeron-Sanchez, M. (Manuel)|||/items/daa40eda-40f4-4474-98fc-98ffe3f84a0f, Prosper-Cardoso, F. (Felipe)|||/items/3d1b0b82-06c3-4e63-8280-e903dc4dc0c1, Muiños-López, E. (Emma)|||/items/73d964b2-f779-4a9a-8f16-79f0f062b620, Granero-Moltó, F. (Froilán)|||/items/9e969359-ca9a-4998-8e4a-bdf4037cba1a
Tipo de documento: artigo
Data de publicação:2023
País:España
Recursos:Universidad de Navarra
Repositório:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglês
OAI Identifier:oai:dadun.unav.edu:10171/67857
Acesso em linha:https://hdl.handle.net/10171/67857
Access Level:Acceso aberto
Palavra-chave:Área de Biomedicina
Induced membrane technique.
Growth-factor delivery.
Stem-cells.
Morphogenetic protein-2.
Biomimetic periosteum.
Defects.
BMP-2
Rat.
Model
Differentiation
Descrição
Resumo:During bone regeneration, the periosteum acts as a carrier for key regenerative cues, delivering osteochondroprogenitor cells and crucial growth factors to the injured bone. We developed a biocompatible, 3D polycaprolactone (PCL) melt electro-written membrane to act as a mimetic periosteum. Poly (ethyl acrylate) coating of the PCL membrane allowed functionalization, mediated by fibronectin and low dose recombinant human BMP-2 (rhBMP-2) (10-25 mu g/ml), resulting in efficient, sustained osteoinduction in vitro. In vivo, rhBMP-2 functionalized mimetic periosteum demonstrated regenerative potential in the treatment of rat critical-size femoral defects with highly efficient healing and functional recovery (80%-93%). Mimetic periosteum has also proven to be efficient for cell delivery, as observed through the migration of transplanted periosteum-derived mesenchymal cells to the bone defect and their survival. Ultimately, mimetic periosteum demonstrated its ability to deliver key stem cells and morphogens to an injured site, exposing a therapeutic and translational potential in vivo when combined with unprecedentedly low rhBMP-2 doses.

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