Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria

The antibacterial activity against bacterial plant pathogens and its relationships with the presence of the cyclic lipopeptide (cLP) biosynthetic genes ituC (iturin), bmyB (bacillomycin), fenD (fengycin) and srfAA (surfactin), and their corresponding antimicrobial peptide products have been studied...

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Autores: Mora Pons, Isabel, Cabrefiga Olamendi, Jordi, Montesinos Seguí, Emilio
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2015
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/13307
Acceso en línea:http://hdl.handle.net/10256/13307
Access Level:acceso abierto
Palabra clave:Antibiòtics pèptids
Peptide antibiotics
Bacils
Bacillus (Bacteria)
Plantes -- Malalties
Plant diseases
Bacteris fitopatògens
Phytopathogenic bacteria
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spelling Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic BacteriaMora Pons, IsabelCabrefiga Olamendi, JordiMontesinos Seguí, EmilioAntibiòtics pèptidsPeptide antibioticsBacilsBacillus (Bacteria)Plantes -- MalaltiesPlant diseasesBacteris fitopatògensPhytopathogenic bacteriaThe antibacterial activity against bacterial plant pathogens and its relationships with the presence of the cyclic lipopeptide (cLP) biosynthetic genes ituC (iturin), bmyB (bacillomycin), fenD (fengycin) and srfAA (surfactin), and their corresponding antimicrobial peptide products have been studied in a collection of 64 strains of Bacillus spp. isolated from plant environments. The most frequent antimicrobial peptide (AMP) genes were bmyB, srfAAand fenD (34-50% of isolates). Most isolates (98.4%) produced surfactin isoforms, 90.6%iturins and 79.7% fengycins. The antibacterial activity was very frequent and generally intense among the collection of strains because 75% of the isolates were active against at least 6 of the 8 bacterial plant pathogens tested. Hierarchical and correspondence analysis confirmed the presence of two clearly differentiated groups. One group consisted of Bacillus strains that showed a strong antibacterial activity, presented several cLPs genes and produced several isoforms of cLPs simultaneously, mainly composed of B. subtilisand B. amyloliquefaciens, although the last one was exclusive to this group. Another group was characterized by strains with very low or none antibacterial activity, that showed one or none of the cLP genes and produced a few or none of the corresponding cLPs, and was the most heterogenous group including B. subtilis, B. licheniformis, B. megaterium, B. pumilus, B. cereusand B. thuringiensis, although the last two were exclusive to this group. This work demonstrated that the antagonistic capacity of plant-associated Bacillusagainst plant pathogenic bacteria is related to the presence of cLP genes and to the production of the corresponding cLPs, and it is mainly associated to the species B. subtilisand B. amyloliquefaciens. Our findings would help to increase the yield and efficiency of screening methods to obtain candidate strains to biocontrol agents with a mechanism of action relaying on the production of antimicrobial cLPsFunding was provided by Programa Nacional de Agricultura y Alimentación (CICYT) (Ref. AGL-2009-13255-C02-01) of the MINECO of Spain, and FP7 KBBE.2013.1.2-04. Ref. 613678. DROPSA project of the European Union. The research group is accredited and supported in part by SGR 2009-0812, 2014 SGR 697 and TECNIO net from CataloniaPublic Library of Science (PLoS)Ministerio de Ciencia e Innovación (Espanya)2015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10256/13307http://hdl.handle.net/10256/13307PLoS One, 2015, vol. 10, núm. 5, p. e0127738Articles publicats (D-EQATA)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0127738info:eu-repo/semantics/altIdentifier/eissn/1932-6203info:eu-repo/grantAgreement/MICINN//AGL2009-13255-C02-01info:eu-repo/grantAgreement/EC/FP7/613678Attribution 4.0 Spainhttp://creativecommons.org/licenses/by/4.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/133072026-05-29T05:05:01Z
dc.title.none.fl_str_mv Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
title Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
spellingShingle Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
Mora Pons, Isabel
Antibiòtics pèptids
Peptide antibiotics
Bacils
Bacillus (Bacteria)
Plantes -- Malalties
Plant diseases
Bacteris fitopatògens
Phytopathogenic bacteria
title_short Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
title_full Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
title_fullStr Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
title_full_unstemmed Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
title_sort Cyclic Lipopeptide Biosynthetic Genes and Products, and Inhibitory Activity of Plant- Associated Bacillus against Phytopathogenic Bacteria
dc.creator.none.fl_str_mv Mora Pons, Isabel
Cabrefiga Olamendi, Jordi
Montesinos Seguí, Emilio
author Mora Pons, Isabel
author_facet Mora Pons, Isabel
Cabrefiga Olamendi, Jordi
Montesinos Seguí, Emilio
author_role author
author2 Cabrefiga Olamendi, Jordi
Montesinos Seguí, Emilio
author2_role author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (Espanya)
dc.subject.none.fl_str_mv Antibiòtics pèptids
Peptide antibiotics
Bacils
Bacillus (Bacteria)
Plantes -- Malalties
Plant diseases
Bacteris fitopatògens
Phytopathogenic bacteria
topic Antibiòtics pèptids
Peptide antibiotics
Bacils
Bacillus (Bacteria)
Plantes -- Malalties
Plant diseases
Bacteris fitopatògens
Phytopathogenic bacteria
description The antibacterial activity against bacterial plant pathogens and its relationships with the presence of the cyclic lipopeptide (cLP) biosynthetic genes ituC (iturin), bmyB (bacillomycin), fenD (fengycin) and srfAA (surfactin), and their corresponding antimicrobial peptide products have been studied in a collection of 64 strains of Bacillus spp. isolated from plant environments. The most frequent antimicrobial peptide (AMP) genes were bmyB, srfAAand fenD (34-50% of isolates). Most isolates (98.4%) produced surfactin isoforms, 90.6%iturins and 79.7% fengycins. The antibacterial activity was very frequent and generally intense among the collection of strains because 75% of the isolates were active against at least 6 of the 8 bacterial plant pathogens tested. Hierarchical and correspondence analysis confirmed the presence of two clearly differentiated groups. One group consisted of Bacillus strains that showed a strong antibacterial activity, presented several cLPs genes and produced several isoforms of cLPs simultaneously, mainly composed of B. subtilisand B. amyloliquefaciens, although the last one was exclusive to this group. Another group was characterized by strains with very low or none antibacterial activity, that showed one or none of the cLP genes and produced a few or none of the corresponding cLPs, and was the most heterogenous group including B. subtilis, B. licheniformis, B. megaterium, B. pumilus, B. cereusand B. thuringiensis, although the last two were exclusive to this group. This work demonstrated that the antagonistic capacity of plant-associated Bacillusagainst plant pathogenic bacteria is related to the presence of cLP genes and to the production of the corresponding cLPs, and it is mainly associated to the species B. subtilisand B. amyloliquefaciens. Our findings would help to increase the yield and efficiency of screening methods to obtain candidate strains to biocontrol agents with a mechanism of action relaying on the production of antimicrobial cLPs
publishDate 2015
dc.date.none.fl_str_mv 2015
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/13307
http://hdl.handle.net/10256/13307
url http://hdl.handle.net/10256/13307
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0127738
info:eu-repo/semantics/altIdentifier/eissn/1932-6203
info:eu-repo/grantAgreement/MICINN//AGL2009-13255-C02-01
info:eu-repo/grantAgreement/EC/FP7/613678
dc.rights.none.fl_str_mv Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 Spain
http://creativecommons.org/licenses/by/4.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv PLoS One, 2015, vol. 10, núm. 5, p. e0127738
Articles publicats (D-EQATA)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,811543

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