Cell-type specification at criticality underlies rhizoid patterning in the liverwort Marchantia polymorpha

Rhizoid specification in Marchantia polymorpha gemmae involves lateral inhibition mediated by the microRNA FRH1, which represses the rhizoid-specific transcription factor RSL1. Accordingly, rhizoid precursor cells appear isolated or spatially organized into small linear clusters. Yet, the molecular...

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Detalles Bibliográficos
Autores: Mercadal, Josep, Ferreira, Mar, Caño-Delgado, Ana I., Ibañes, Marta
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2026
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/422945
Acceso en línea:http://hdl.handle.net/10261/422945
https://api.elsevier.com/content/abstract/scopus_id/105029055667
Access Level:acceso abierto
Palabra clave:Rhizoids
Cell specification
Criticality
Lateral inhibition
Marchantia
Pattern formation
Reaction-diffusion
Descripción
Sumario:Rhizoid specification in Marchantia polymorpha gemmae involves lateral inhibition mediated by the microRNA FRH1, which represses the rhizoid-specific transcription factor RSL1. Accordingly, rhizoid precursor cells appear isolated or spatially organized into small linear clusters. Yet, the molecular mechanisms driving this patterning remain unclear. Using mathematical modeling, we show that rhizoid patterning is consistent with local activation of RSL1, modulated by lateral inhibition through FRH1 diffusion. Our results suggest that rhizoid precursor cells are initiated stochastically and refined by lateral inhibition via a diffusion-driven switch, in which FRH1 mobility generates a sharp transition between spatially uniform and patterned states. These patterns are associated with a subcritical Turing bifurcation and naturally occur at criticality. Combining published and original data, we show that our model can reproduce the spatial statistics of wild-type and mutant phenotypes. Our work highlights a patterning mechanism underlying cellular differentiation and tissue morphogenesis.