Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea

Rotavirus (RV) is the main cause of gastroenteritis in children. Prebiotics and, more recently, postbiotics are used for preventing and treating gastrointestinal infections. The aim of this study was to analyze the effects of a LactofidusTM, short-chain galacto-oligosaccharides (scGOS) and long-chai...

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Autores: Morales Ferré, Carla, Azagra Boronat, Ignasi, Massot Cladera, Malen, Tims, S., Knipping, K., Garssen, J., Knol, J., Franch i Masferrer, Àngels, Castell, Margarida, Pérez-Cano, Francisco J., Rodríguez Lagunas, María José
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/185957
Acceso en línea:https://hdl.handle.net/2445/185957
Access Level:acceso abierto
Palabra clave:Diarrea
Malalties intestinals
Prebiòtics
Probiòtics
Diarrhea
Intestinal diseases
Prebiotics
Probiotics
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spelling Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-DiarrheaMorales Ferré, CarlaAzagra Boronat, IgnasiMassot Cladera, MalenTims, S.Knipping, K.Garssen, J.Knol, J.Franch i Masferrer, ÀngelsCastell, MargaridaPérez-Cano, Francisco J.Rodríguez Lagunas, María JoséDiarreaMalalties intestinalsPrebiòticsProbiòticsDiarrheaIntestinal diseasesPrebioticsProbioticsRotavirus (RV) is the main cause of gastroenteritis in children. Prebiotics and, more recently, postbiotics are used for preventing and treating gastrointestinal infections. The aim of this study was to analyze the effects of a LactofidusTM, short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharides (lcFOS) mixture, and their combination on RV infection, in a rat model, for early life diarrhea. Fifteen litters of suckling rats were intragastrically administered daily with the vehicle, the prebiotic mixture, the postbiotic or the combination. The RV was inoculated on day 5 and then fecal samples were clinically evaluated daily. Viral shedding, intestinal permeability assay, in vitro blocking assay, immunoglobulin profiles, and anti-RV response were assessed at day 8 and 16 of life. Cecal microbiota composition, intestinal gene expression, and short chain fatty acids (SCFAs) were analyzed at day 16. The incidence and severity of diarrhea were significantly reduced by all the supplementations. Moreover, they showed blocking activity, changes in the immunoglobulin profiles, in gut microbiota, and in the intestinal gene expression. The prebiotic mixture reduced gut permeability and changed the SCFA profile, whereas the postbiotic enhanced the expression of Toll-like receptors (TLRs). The combination preserved most of the individual observed effects, and furthermore, complementary effects, such as an increase in white blood cells and lymphocytes recruitment, as well as upregulation of TLR7 and TLR9 gene expression.MDPI2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/185957Articles publicats en revistes (Bioquímica i Fisiologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.3390/nu14061163Nutrients, 2022, vol. 14, num. 6, p. 1-23https://doi.org/10.3390/nu14061163cc-by (c) Morales Ferré, Carla et al., 2022https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1859572026-05-27T06:46:51Z
dc.title.none.fl_str_mv Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
title Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
spellingShingle Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
Morales Ferré, Carla
Diarrea
Malalties intestinals
Prebiòtics
Probiòtics
Diarrhea
Intestinal diseases
Prebiotics
Probiotics
title_short Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
title_full Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
title_fullStr Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
title_full_unstemmed Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
title_sort Preventive Effect of a Postbiotic and Prebiotic Mixture in a Rat Model of Early Life Rotavirus Induced-Diarrhea
dc.creator.none.fl_str_mv Morales Ferré, Carla
Azagra Boronat, Ignasi
Massot Cladera, Malen
Tims, S.
Knipping, K.
Garssen, J.
Knol, J.
Franch i Masferrer, Àngels
Castell, Margarida
Pérez-Cano, Francisco J.
Rodríguez Lagunas, María José
author Morales Ferré, Carla
author_facet Morales Ferré, Carla
Azagra Boronat, Ignasi
Massot Cladera, Malen
Tims, S.
Knipping, K.
Garssen, J.
Knol, J.
Franch i Masferrer, Àngels
Castell, Margarida
Pérez-Cano, Francisco J.
Rodríguez Lagunas, María José
author_role author
author2 Azagra Boronat, Ignasi
Massot Cladera, Malen
Tims, S.
Knipping, K.
Garssen, J.
Knol, J.
Franch i Masferrer, Àngels
Castell, Margarida
Pérez-Cano, Francisco J.
Rodríguez Lagunas, María José
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Diarrea
Malalties intestinals
Prebiòtics
Probiòtics
Diarrhea
Intestinal diseases
Prebiotics
Probiotics
topic Diarrea
Malalties intestinals
Prebiòtics
Probiòtics
Diarrhea
Intestinal diseases
Prebiotics
Probiotics
description Rotavirus (RV) is the main cause of gastroenteritis in children. Prebiotics and, more recently, postbiotics are used for preventing and treating gastrointestinal infections. The aim of this study was to analyze the effects of a LactofidusTM, short-chain galacto-oligosaccharides (scGOS) and long-chain fructo-oligosaccharides (lcFOS) mixture, and their combination on RV infection, in a rat model, for early life diarrhea. Fifteen litters of suckling rats were intragastrically administered daily with the vehicle, the prebiotic mixture, the postbiotic or the combination. The RV was inoculated on day 5 and then fecal samples were clinically evaluated daily. Viral shedding, intestinal permeability assay, in vitro blocking assay, immunoglobulin profiles, and anti-RV response were assessed at day 8 and 16 of life. Cecal microbiota composition, intestinal gene expression, and short chain fatty acids (SCFAs) were analyzed at day 16. The incidence and severity of diarrhea were significantly reduced by all the supplementations. Moreover, they showed blocking activity, changes in the immunoglobulin profiles, in gut microbiota, and in the intestinal gene expression. The prebiotic mixture reduced gut permeability and changed the SCFA profile, whereas the postbiotic enhanced the expression of Toll-like receptors (TLRs). The combination preserved most of the individual observed effects, and furthermore, complementary effects, such as an increase in white blood cells and lymphocytes recruitment, as well as upregulation of TLR7 and TLR9 gene expression.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/185957
url https://hdl.handle.net/2445/185957
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.3390/nu14061163
Nutrients, 2022, vol. 14, num. 6, p. 1-23
https://doi.org/10.3390/nu14061163
dc.rights.none.fl_str_mv cc-by (c) Morales Ferré, Carla et al., 2022
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Morales Ferré, Carla et al., 2022
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Fisiologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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