Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm

Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the...

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Authors: Llamazares Cantalejo, Celia, Sanz del Olmo, Natalia, Ortega Lopez, Paula|||0000-0003-0377-5429, Gómez Ramírez, Rafael|||0000-0001-6448-2414, Soliveri de Carranza, Juan|||0000-0001-8200-0132, Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935, García Gallego, Sandra|||0000-0001-6112-0450, Copa Patiño, José Luis|||0000-0002-2162-3907
Format: article
Publication Date:2019
Country:España
Institution:Universidad de Alcalá (UAH)
Repository:e_Buah Biblioteca Digital Universidad de Alcalá
Language:English
OAI Identifier:oai:ebuah.uah.es:10017/39787
Online Access:http://hdl.handle.net/10017/39787
https://dx.doi.org/10.3390/biom9090405
Access Level:Open access
Keyword:dendrimer
metallodendrimer
metal
copper
ruthenium
antibacterial
biofilm
Staphylococcus aureus
Escherichia coli
Biología y Biomedicina/Biología
Química
Biology
Chemistry
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spelling Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus BiofilmLlamazares Cantalejo, CeliaSanz del Olmo, NataliaOrtega Lopez, Paula|||0000-0003-0377-5429Gómez Ramírez, Rafael|||0000-0001-6448-2414Soliveri de Carranza, Juan|||0000-0001-8200-0132Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935García Gallego, Sandra|||0000-0001-6112-0450Copa Patiño, José Luis|||0000-0002-2162-3907dendrimermetallodendrimermetalcopperrutheniumantibacterialbiofilmStaphylococcus aureusEscherichia coliBiología y Biomedicina/BiologíaQuímicaBiologyChemistryAntibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the antibacterial effect of carbosilane metallodendrimers with different metals (copper(II)and ruthenium(II)), ligands (chloride and nitrate) and generations (generation 0, 1 and 2) has been studied using planktonic Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Furthermore, the ability of the metallodendrimers to avoid the formation of S. aureus biofilms was also evaluated. The results showed a promising biocide activity in both types of planktonic bacteria, especially for first-generation dendrimers, which arises from the metal complexation to the dendrimer. Cu(II) metallodendrimers require lower concentration than Ru(II) counterpart to inhibit the production of S. aureus biofilms, but none produce hemolysis at the inhibitory concentrations andcan be safely used as antibacterial agents. In particular, the first-generation Cu(II) metallodendrimer with nitrate ligands displayed the most promising properties to continue with further studies in both planktonic cells and biofilms.Agencia Estatal de InvestigaciónJunta de Comunidades de Castilla-La ManchaComunidad de Madrid20192019-08-23journal articlehttp://purl.org/coar/resource_type/c_6501NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10017/39787https://dx.doi.org/10.3390/biom9090405reponame:e_Buah Biblioteca Digital Universidad de Alcaláinstname:Universidad de Alcalá (UAH)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 CTQ2017-86224-P Dendronización como herramienta sintética de obtención de nanosistemas dendríticos específicamente diseñados para uso en aplicaciones biomédicasComunidad de Madrid http://dx.doi.org/10.13039/100012818 Programas de Actividades de I+D entre Grupos de Investigación de la Comunidad de Madrid en Biomedicina B2017%2FBMD-3733 Inmunidad tumoral e inmunoterapia del cáncerComunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703 Nanosistemas dendríticos como agentes y vectores terapéuticos en distintas aplicaciones biomédicas (II)Junta de Comunidades de Castilla-La Mancha http://dx.doi.org/10.13039/501100011698 Not available SBPLY%2F17%2F180501%2F000358 Nanosistemas dendríticos polifenólicos: aplicaciones en cosmética y biomedicinaopen accesshttp://purl.org/coar/access_right/c_abf2Attribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ebuah.uah.es:10017/397872026-06-18T11:13:07Z
dc.title.none.fl_str_mv Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
spellingShingle Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
Llamazares Cantalejo, Celia
dendrimer
metallodendrimer
metal
copper
ruthenium
antibacterial
biofilm
Staphylococcus aureus
Escherichia coli
Biología y Biomedicina/Biología
Química
Biology
Chemistry
title_short Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_full Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_fullStr Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_full_unstemmed Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
title_sort Antibacterial Effect of Carbosilane Metallodendrimers in Planktonic Cells of Gram-Positive and Gram-Negative Bacteria and Staphylococcus aureus Biofilm
dc.creator.none.fl_str_mv Llamazares Cantalejo, Celia
Sanz del Olmo, Natalia
Ortega Lopez, Paula|||0000-0003-0377-5429
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
García Gallego, Sandra|||0000-0001-6112-0450
Copa Patiño, José Luis|||0000-0002-2162-3907
author Llamazares Cantalejo, Celia
author_facet Llamazares Cantalejo, Celia
Sanz del Olmo, Natalia
Ortega Lopez, Paula|||0000-0003-0377-5429
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
García Gallego, Sandra|||0000-0001-6112-0450
Copa Patiño, José Luis|||0000-0002-2162-3907
author_role author
author2 Sanz del Olmo, Natalia
Ortega Lopez, Paula|||0000-0003-0377-5429
Gómez Ramírez, Rafael|||0000-0001-6448-2414
Soliveri de Carranza, Juan|||0000-0001-8200-0132
Mata de la Mata, Francisco Javier de la|||0000-0003-0418-3935
García Gallego, Sandra|||0000-0001-6112-0450
Copa Patiño, José Luis|||0000-0002-2162-3907
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv dendrimer
metallodendrimer
metal
copper
ruthenium
antibacterial
biofilm
Staphylococcus aureus
Escherichia coli
Biología y Biomedicina/Biología
Química
Biology
Chemistry
topic dendrimer
metallodendrimer
metal
copper
ruthenium
antibacterial
biofilm
Staphylococcus aureus
Escherichia coli
Biología y Biomedicina/Biología
Química
Biology
Chemistry
description Antibiotic resistance is currently one of the main threats to public health security. Biofilm formation is a resistance mechanism that is responsible for most human bacterial infections and requires new and effective therapeutic approaches, such as those provided by nanotechnology. In this work, the antibacterial effect of carbosilane metallodendrimers with different metals (copper(II)and ruthenium(II)), ligands (chloride and nitrate) and generations (generation 0, 1 and 2) has been studied using planktonic Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria. Furthermore, the ability of the metallodendrimers to avoid the formation of S. aureus biofilms was also evaluated. The results showed a promising biocide activity in both types of planktonic bacteria, especially for first-generation dendrimers, which arises from the metal complexation to the dendrimer. Cu(II) metallodendrimers require lower concentration than Ru(II) counterpart to inhibit the production of S. aureus biofilms, but none produce hemolysis at the inhibitory concentrations andcan be safely used as antibacterial agents. In particular, the first-generation Cu(II) metallodendrimer with nitrate ligands displayed the most promising properties to continue with further studies in both planktonic cells and biofilms.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019-08-23
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10017/39787
https://dx.doi.org/10.3390/biom9090405
url http://hdl.handle.net/10017/39787
https://dx.doi.org/10.3390/biom9090405
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016 CTQ2017-86224-P Dendronización como herramienta sintética de obtención de nanosistemas dendríticos específicamente diseñados para uso en aplicaciones biomédicas
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Programas de Actividades de I+D entre Grupos de Investigación de la Comunidad de Madrid en Biomedicina B2017%2FBMD-3733 Inmunidad tumoral e inmunoterapia del cáncer
Comunidad de Madrid http://dx.doi.org/10.13039/100012818 Not available B2017%2FBMD-3703 Nanosistemas dendríticos como agentes y vectores terapéuticos en distintas aplicaciones biomédicas (II)
Junta de Comunidades de Castilla-La Mancha http://dx.doi.org/10.13039/501100011698 Not available SBPLY%2F17%2F180501%2F000358 Nanosistemas dendríticos polifenólicos: aplicaciones en cosmética y biomedicina
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:e_Buah Biblioteca Digital Universidad de Alcalá
instname:Universidad de Alcalá (UAH)
instname_str Universidad de Alcalá (UAH)
reponame_str e_Buah Biblioteca Digital Universidad de Alcalá
collection e_Buah Biblioteca Digital Universidad de Alcalá
repository.name.fl_str_mv
repository.mail.fl_str_mv
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