Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics

Background: The presence of micro- and nanoplastics (MNPLs) in human blood raises concerns about their vascular impact and their potential contribution to cardiovascular diseases. Endothelial cells are a primary target of circulating MNPLs; however, the molecular and functional consequences of this...

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Autores: Martín Pérez, Joan|||0009-0006-9137-3604, Villacorta, Aliro|||0000-0003-2737-4232, Gutierrez Garcia, Javier|||0009-0007-7376-8933, Egea, Raquel|||0000-0002-6201-4112, Morataya Reyes, Michelle|||0000-0001-9620-2069, Cassú-Casadevall, Mireia, Barguilla, Irene|||0000-0003-1081-4457, Marcos Dauder, Ricardo|||0000-0001-7891-357X, Hernández Bonilla, Alba|||0000-0001-6938-1233, García Rodríguez, Alba|||0000-0002-1175-7418
Tipo de recurso: artículo
Fecha de publicación:2026
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:dnet:uabarcelona_::c2eba6d918a0bdb38d08dbc1d721ac7b
Acceso en línea:https://ddd.uab.cat/record/327986
https://dx.doi.org/urn:doi:10.3389/ftox.2026.1812922
Access Level:acceso abierto
Palabra clave:HUVEC
Functional effects
Genotoxicity
Internalization
Polystyrene nanoplastics
Transcriptomics
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spelling Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplasticsMartín Pérez, Joan|||0009-0006-9137-3604Villacorta, Aliro|||0000-0003-2737-4232Gutierrez Garcia, Javier|||0009-0007-7376-8933Egea, Raquel|||0000-0002-6201-4112Morataya Reyes, Michelle|||0000-0001-9620-2069Cassú-Casadevall, MireiaBarguilla, Irene|||0000-0003-1081-4457Marcos Dauder, Ricardo|||0000-0001-7891-357XHernández Bonilla, Alba|||0000-0001-6938-1233García Rodríguez, Alba|||0000-0002-1175-7418HUVECFunctional effectsGenotoxicityInternalizationPolystyrene nanoplasticsTranscriptomicsBackground: The presence of micro- and nanoplastics (MNPLs) in human blood raises concerns about their vascular impact and their potential contribution to cardiovascular diseases. Endothelial cells are a primary target of circulating MNPLs; however, the molecular and functional consequences of this exposure remain largely undefined. Methods: In this study, we exposed primary human umbilical vein endothelial cells (HUVECs) to carboxylated polystyrene nanoplastics (PS-NPLs; 30, 50, and 100 nm) and integrated RNA sequencing with targeted functional assays. Results: Transcriptomics revealed a robust response characterized by coordinated dysregulation of cholesterol homeostasis, genotoxic stress and DNA repair, inflammatory signaling, and endothelial plasticity (endothelial-to-mesenchymal transition). Guided by these signatures, functional assays confirmed increased intracellular cholesterol, DNA damage, remodelling of migratory capacity and angiogenic behaviour, and reduced IL-6 secretion. Discussion: Overall, the concordance between transcriptomic programs and functional endpoints supports a mechanistic framework in which PS-NPL exposure rewires endothelial metabolic and stress-response networks, with downstream consequences for key vascular functions. Differences across the nanoscale range modulated the magnitude and temporal profile of specific endpoints, but the shared molecular core response predominated across treatments. 22026-01-0120262026-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/327986https://dx.doi.org/urn:doi:10.3389/ftox.2026.1812922reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengGeneralitat de Catalunya https://doi.org/10.13039/501100002809 2020/BP-00177Generalitat de Catalunya https://doi.org/10.13039/501100002809 2023/BP-00212European Commission https://doi.org/10.13039/501100000780 965196Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43Generalitat de Catalunya https://doi.org/10.13039/501100002809 2021/SGR-00731open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:uabarcelona_::c2eba6d918a0bdb38d08dbc1d721ac7b2026-06-06T12:50:31Z
dc.title.none.fl_str_mv Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
title Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
spellingShingle Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
Martín Pérez, Joan|||0009-0006-9137-3604
HUVEC
Functional effects
Genotoxicity
Internalization
Polystyrene nanoplastics
Transcriptomics
title_short Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
title_full Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
title_fullStr Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
title_full_unstemmed Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
title_sort Transcriptomic and functional profiling of endothelial dysfunction induced by polystyrene nanoplastics
dc.creator.none.fl_str_mv Martín Pérez, Joan|||0009-0006-9137-3604
Villacorta, Aliro|||0000-0003-2737-4232
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Morataya Reyes, Michelle|||0000-0001-9620-2069
Cassú-Casadevall, Mireia
Barguilla, Irene|||0000-0003-1081-4457
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
García Rodríguez, Alba|||0000-0002-1175-7418
author Martín Pérez, Joan|||0009-0006-9137-3604
author_facet Martín Pérez, Joan|||0009-0006-9137-3604
Villacorta, Aliro|||0000-0003-2737-4232
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Morataya Reyes, Michelle|||0000-0001-9620-2069
Cassú-Casadevall, Mireia
Barguilla, Irene|||0000-0003-1081-4457
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
García Rodríguez, Alba|||0000-0002-1175-7418
author_role author
author2 Villacorta, Aliro|||0000-0003-2737-4232
Gutierrez Garcia, Javier|||0009-0007-7376-8933
Egea, Raquel|||0000-0002-6201-4112
Morataya Reyes, Michelle|||0000-0001-9620-2069
Cassú-Casadevall, Mireia
Barguilla, Irene|||0000-0003-1081-4457
Marcos Dauder, Ricardo|||0000-0001-7891-357X
Hernández Bonilla, Alba|||0000-0001-6938-1233
García Rodríguez, Alba|||0000-0002-1175-7418
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv HUVEC
Functional effects
Genotoxicity
Internalization
Polystyrene nanoplastics
Transcriptomics
topic HUVEC
Functional effects
Genotoxicity
Internalization
Polystyrene nanoplastics
Transcriptomics
description Background: The presence of micro- and nanoplastics (MNPLs) in human blood raises concerns about their vascular impact and their potential contribution to cardiovascular diseases. Endothelial cells are a primary target of circulating MNPLs; however, the molecular and functional consequences of this exposure remain largely undefined. Methods: In this study, we exposed primary human umbilical vein endothelial cells (HUVECs) to carboxylated polystyrene nanoplastics (PS-NPLs; 30, 50, and 100 nm) and integrated RNA sequencing with targeted functional assays. Results: Transcriptomics revealed a robust response characterized by coordinated dysregulation of cholesterol homeostasis, genotoxic stress and DNA repair, inflammatory signaling, and endothelial plasticity (endothelial-to-mesenchymal transition). Guided by these signatures, functional assays confirmed increased intracellular cholesterol, DNA damage, remodelling of migratory capacity and angiogenic behaviour, and reduced IL-6 secretion. Discussion: Overall, the concordance between transcriptomic programs and functional endpoints supports a mechanistic framework in which PS-NPL exposure rewires endothelial metabolic and stress-response networks, with downstream consequences for key vascular functions. Differences across the nanoscale range modulated the magnitude and temporal profile of specific endpoints, but the shared molecular core response predominated across treatments.
publishDate 2026
dc.date.none.fl_str_mv 2
2026-01-01
2026
2026-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/327986
https://dx.doi.org/urn:doi:10.3389/ftox.2026.1812922
url https://ddd.uab.cat/record/327986
https://dx.doi.org/urn:doi:10.3389/ftox.2026.1812922
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Generalitat de Catalunya https://doi.org/10.13039/501100002809 2020/BP-00177
Generalitat de Catalunya https://doi.org/10.13039/501100002809 2023/BP-00212
European Commission https://doi.org/10.13039/501100000780 965196
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2020-116789RB-C43
Generalitat de Catalunya https://doi.org/10.13039/501100002809 2021/SGR-00731
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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