Long non-coding RNAs involved in Drosophila development and regeneration

The discovery of functional long non-coding RNAs (lncRNAs) changed their initial concept as transcriptional noise. LncRNAs have been identified as regulators of multiple biological processes, including chromatin structure, gene expression, splicing, mRNA degradation, and translation. However, functi...

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Detalles Bibliográficos
Autores: Camilleri Robles, Carlos, Amador, Raziel, Tiebe, Marcel, Teleman, Aurelio A., Serras, Florenci, Guigó Serra, Roderic, Corominas Guiu, Montserrat
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Universitat Pompeu Fabra
Repositorio:Repositorio Digital de la UPF
OAI Identifier:oai:repositori.upf.edu:10230/61175
Acceso en línea:http://hdl.handle.net/10230/61175
http://dx.doi.org/10.1093/nargab/lqae091
Access Level:acceso abierto
Palabra clave:RNA
Drosòfila--Genètica
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spelling Long non-coding RNAs involved in Drosophila development and regenerationCamilleri Robles, CarlosAmador, RazielTiebe, MarcelTeleman, Aurelio A.Serras, FlorenciGuigó Serra, RodericCorominas Guiu, MontserratRNADrosòfila--GenèticaThe discovery of functional long non-coding RNAs (lncRNAs) changed their initial concept as transcriptional noise. LncRNAs have been identified as regulators of multiple biological processes, including chromatin structure, gene expression, splicing, mRNA degradation, and translation. However, functional studies of lncRNAs are hindered by the usual lack of phenotypes upon deletion or inhibition. Here, we used Drosophila imaginal discs as a model system to identify lncRNAs involved in development and regeneration. We examined a subset of lncRNAs expressed in the wing, leg, and eye disc development. Additionally, we analyzed transcriptomic data from regenerating wing discs to profile the expression pattern of lncRNAs during tissue repair. We focused on the lncRNA CR40469, which is upregulated during regeneration. We generated CR40469 mutant flies that developed normally but showed impaired wing regeneration upon cell death induction. The ability of these mutants to regenerate was restored by the ectopic expression of CR40469. Furthermore, we found that the lncRNA CR34335 has a high degree of sequence similarity with CR40469 and can partially compensate for its function during regeneration in the absence of CR40469. Our findings point to a potential role of the lncRNA CR40469 in trans during the response to damage in the wing imaginal disc.Ministerio de Ciencia e Innovación of the Spanish Government [PGC2018-099763-B100, PID2021-123300NB-I00, BES-2016-076691]; Agència de Gestió d’Ajuts Universitaris i Recerca of the Generalitat de Catalunya [2021SGR00293]; Institució Catalana de Recerca i Estudis Avançats (via an ICREA Academia award to M.C.); New Indigo project (to A.A.T.); Consejo Nacional de Ciencia y Tecnología of the Mexican Government (predoctoral fellowship awarded to R.A.).Oxford University Press202420242024info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/61175http://dx.doi.org/10.1093/nargab/lqae091reponame:Repositorio Digital de la UPFinstname:Universitat Pompeu FabraInglésNAR Genom Bioinform. 2024 Aug 16;6(3):lqae091info:eu-repo/grantAgreement/ES/2PE/PGC2018-099763-B100info:eu-repo/grantAgreement/ES/3PE/PID2021-123300NB-I00info:eu-repo/grantAgreement/ES/1PE/BES-2016-076691© The Author(s) 2024. Published by Oxford University Press on behalf of NAR Genomics and Bioinformatics. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:repositori.upf.edu:10230/611752026-06-12T07:21:37Z
dc.title.none.fl_str_mv Long non-coding RNAs involved in Drosophila development and regeneration
title Long non-coding RNAs involved in Drosophila development and regeneration
spellingShingle Long non-coding RNAs involved in Drosophila development and regeneration
Camilleri Robles, Carlos
RNA
Drosòfila--Genètica
title_short Long non-coding RNAs involved in Drosophila development and regeneration
title_full Long non-coding RNAs involved in Drosophila development and regeneration
title_fullStr Long non-coding RNAs involved in Drosophila development and regeneration
title_full_unstemmed Long non-coding RNAs involved in Drosophila development and regeneration
title_sort Long non-coding RNAs involved in Drosophila development and regeneration
dc.creator.none.fl_str_mv Camilleri Robles, Carlos
Amador, Raziel
Tiebe, Marcel
Teleman, Aurelio A.
Serras, Florenci
Guigó Serra, Roderic
Corominas Guiu, Montserrat
author Camilleri Robles, Carlos
author_facet Camilleri Robles, Carlos
Amador, Raziel
Tiebe, Marcel
Teleman, Aurelio A.
Serras, Florenci
Guigó Serra, Roderic
Corominas Guiu, Montserrat
author_role author
author2 Amador, Raziel
Tiebe, Marcel
Teleman, Aurelio A.
Serras, Florenci
Guigó Serra, Roderic
Corominas Guiu, Montserrat
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv RNA
Drosòfila--Genètica
topic RNA
Drosòfila--Genètica
description The discovery of functional long non-coding RNAs (lncRNAs) changed their initial concept as transcriptional noise. LncRNAs have been identified as regulators of multiple biological processes, including chromatin structure, gene expression, splicing, mRNA degradation, and translation. However, functional studies of lncRNAs are hindered by the usual lack of phenotypes upon deletion or inhibition. Here, we used Drosophila imaginal discs as a model system to identify lncRNAs involved in development and regeneration. We examined a subset of lncRNAs expressed in the wing, leg, and eye disc development. Additionally, we analyzed transcriptomic data from regenerating wing discs to profile the expression pattern of lncRNAs during tissue repair. We focused on the lncRNA CR40469, which is upregulated during regeneration. We generated CR40469 mutant flies that developed normally but showed impaired wing regeneration upon cell death induction. The ability of these mutants to regenerate was restored by the ectopic expression of CR40469. Furthermore, we found that the lncRNA CR34335 has a high degree of sequence similarity with CR40469 and can partially compensate for its function during regeneration in the absence of CR40469. Our findings point to a potential role of the lncRNA CR40469 in trans during the response to damage in the wing imaginal disc.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/61175
http://dx.doi.org/10.1093/nargab/lqae091
url http://hdl.handle.net/10230/61175
http://dx.doi.org/10.1093/nargab/lqae091
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv NAR Genom Bioinform. 2024 Aug 16;6(3):lqae091
info:eu-repo/grantAgreement/ES/2PE/PGC2018-099763-B100
info:eu-repo/grantAgreement/ES/3PE/PID2021-123300NB-I00
info:eu-repo/grantAgreement/ES/1PE/BES-2016-076691
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositorio Digital de la UPF
instname:Universitat Pompeu Fabra
instname_str Universitat Pompeu Fabra
reponame_str Repositorio Digital de la UPF
collection Repositorio Digital de la UPF
repository.name.fl_str_mv
repository.mail.fl_str_mv
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