Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp

Streptomyces bacteria produce a wide number of antibiotics and antitumor compounds that have attracted the attention of pharmaceutical and biotech companies. In this study, we provide evidence showing that the xylem sap from grapevines has a positive effect on the production of different antibiotics...

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Autores: Santamaría, Ramón I., Martínez-Carrasco, Ana, Martín, Jesús, Tormo, José R., Pérez-Victoria, Ignacio, González, Ignacio, Genilloud, Olga, Reyes, Fernando, Díaz, Margarita
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/281525
Acceso en línea:http://hdl.handle.net/10261/281525
Access Level:acceso abierto
Palabra clave:Streptomyces
Elicitor
Antibiotic production
Xylem sap
Grapevine
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spelling Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces sppSantamaría, Ramón I.Martínez-Carrasco, AnaMartín, JesúsTormo, José R.Pérez-Victoria, IgnacioGonzález, IgnacioGenilloud, OlgaReyes, FernandoDíaz, MargaritaStreptomycesElicitorAntibiotic productionXylem sapGrapevineStreptomyces bacteria produce a wide number of antibiotics and antitumor compounds that have attracted the attention of pharmaceutical and biotech companies. In this study, we provide evidence showing that the xylem sap from grapevines has a positive effect on the production of different antibiotics by several Streptomyces species, including S. ambofaciens ATCC 23877 and S. argillaceus ATCC 12596 among others. The production of several already known compounds was induced: actinomycin D, chromomycin A3, fungichromin B, mithramycin A, etc., and four compounds with molecular formulas not included in the Dictionary of Natural Products (DNP v28.2) were also produced. The molecules present in the xylem sap that acts as elicitors were smaller than 3 kDa and soluble in water and insoluble in ether, ethyl acetate, or methanol. A combination of potassium citrate and di-D-fructose dianhydrides (related to levanbiose or inulobiose) seemed to be the main effectors identified from the active fraction. However, the level of induction obtained in the presence of these compounds mix was weaker and delayed with respect to the one got when using the whole xylem sap or the 3 kDa sap fraction, suggesting that another, not identified, elicitor must be also implied in this inductionThe laboratory of R.I.S. and M.D. is funded by projects PID2019-107716RB-I00 (MICN/AEI/10.13039/501100011033), SA036G19 (“Consejería de Educación” of Regional Government of Castile and Leon) and, from the Regional Government of Castile and Leon/FEDER program (“Escalera de Excelencia” CLU-2017-03/14-20) to the Institute of Functional Biology and Genomics (IBFG). CLU-2017-03 co-funded by the P.O. FEDER of Castilla y León 14-20.Multidisciplinary Digital Publishing InstituteMinisterio de Ciencia e Innovación (España)Agencia Estatal de Investigación (España)Junta de Castilla y LeónEuropean CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2022202220222022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/281525reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107716RB-I00https:// doi.org/10.3390/antibiotics11050672Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2815252026-05-22T06:33:51Z
dc.title.none.fl_str_mv Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
title Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
spellingShingle Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
Santamaría, Ramón I.
Streptomyces
Elicitor
Antibiotic production
Xylem sap
Grapevine
title_short Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
title_full Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
title_fullStr Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
title_full_unstemmed Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
title_sort Grapevine Xylem Sap Is a Potent Elicitor of Antibiotic Production in Streptomyces spp
dc.creator.none.fl_str_mv Santamaría, Ramón I.
Martínez-Carrasco, Ana
Martín, Jesús
Tormo, José R.
Pérez-Victoria, Ignacio
González, Ignacio
Genilloud, Olga
Reyes, Fernando
Díaz, Margarita
author Santamaría, Ramón I.
author_facet Santamaría, Ramón I.
Martínez-Carrasco, Ana
Martín, Jesús
Tormo, José R.
Pérez-Victoria, Ignacio
González, Ignacio
Genilloud, Olga
Reyes, Fernando
Díaz, Margarita
author_role author
author2 Martínez-Carrasco, Ana
Martín, Jesús
Tormo, José R.
Pérez-Victoria, Ignacio
González, Ignacio
Genilloud, Olga
Reyes, Fernando
Díaz, Margarita
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Agencia Estatal de Investigación (España)
Junta de Castilla y León
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Streptomyces
Elicitor
Antibiotic production
Xylem sap
Grapevine
topic Streptomyces
Elicitor
Antibiotic production
Xylem sap
Grapevine
description Streptomyces bacteria produce a wide number of antibiotics and antitumor compounds that have attracted the attention of pharmaceutical and biotech companies. In this study, we provide evidence showing that the xylem sap from grapevines has a positive effect on the production of different antibiotics by several Streptomyces species, including S. ambofaciens ATCC 23877 and S. argillaceus ATCC 12596 among others. The production of several already known compounds was induced: actinomycin D, chromomycin A3, fungichromin B, mithramycin A, etc., and four compounds with molecular formulas not included in the Dictionary of Natural Products (DNP v28.2) were also produced. The molecules present in the xylem sap that acts as elicitors were smaller than 3 kDa and soluble in water and insoluble in ether, ethyl acetate, or methanol. A combination of potassium citrate and di-D-fructose dianhydrides (related to levanbiose or inulobiose) seemed to be the main effectors identified from the active fraction. However, the level of induction obtained in the presence of these compounds mix was weaker and delayed with respect to the one got when using the whole xylem sap or the 3 kDa sap fraction, suggesting that another, not identified, elicitor must be also implied in this induction
publishDate 2022
dc.date.none.fl_str_mv 2022
2022
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/281525
url http://hdl.handle.net/10261/281525
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107716RB-I00
https:// doi.org/10.3390/antibiotics11050672
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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