Injectable alginate hydrogel loaded with GDNF promotes functional recovery in a hemisection model of spinal cord injury

We hypothesized that local delivery of GDNF in spinal cord lesion via an injectable alginate hydrogel gelifying in situ would support spinal cord plasticity and functional recovery. The GDNF release from the hydrogel was slowed by GDNF encapsulation in microspheres compared to non-formulated GDNF (f...

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Detalles Bibliográficos
Autores: Ansorena-Artieda, E. (Eduardo)|||/items/eee6c2d6-f73a-48d3-a5f8-fe090b677c82, Berdt, P. (Pauline) de|||/items/db7ad076-fbf7-4038-8429-ac11864471ce, Ucakar, B. (Bernard)|||/items/655f57ae-f748-47f3-ad02-9620d2707eb7, Simon-Yarza, T. (Teresa)|||/items/8bf17254-4031-4c72-9a3a-9622a60c311f, Jacobs, D. (Damien)|||/items/436d5e29-1fc0-471e-990f-f559ba457a59, Schakman, O. (Olivier)|||/items/7ee316ac-8165-4a88-8abb-538e6f4a41ab, Jankovski, A. (Aleksandar)|||/items/297f013a-383f-43e3-9633-b887b06735af, Deumens, R. (Ronald)|||/items/a0a66dd7-1d88-4a9a-a9a8-9edd51210c08, Blanco-Prieto, M.J. (María José)|||/items/93e177db-635f-456f-b672-b79ef8befc40, Preat, V. (Veronique)|||/items/0a2c09ed-3326-4294-b977-2ff01de90bce, Rieux, A. (Anne) des|||/items/5f717b00-81c4-4e69-9f6a-4770e1bfdda3
Tipo de recurso: artículo
Fecha de publicación:2013
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/34791
Acceso en línea:https://hdl.handle.net/10171/34791
Access Level:acceso abierto
Palabra clave:GDNF
Microspheres
Spinal cord injury
Injectable hydrogel
Alginate
Descripción
Sumario:We hypothesized that local delivery of GDNF in spinal cord lesion via an injectable alginate hydrogel gelifying in situ would support spinal cord plasticity and functional recovery. The GDNF release from the hydrogel was slowed by GDNF encapsulation in microspheres compared to non-formulated GDNF (free GDNF). When injected in a rat spinal cord hemisection model, more neurofilaments were observed in the lesion when the rats were treated with free GDNF-loaded hydrogels. More growing neurites were detected in the tissues surrounding the lesion when the animals were treated with GDNF microsphere-loaded hydrogels. Intense GFAP (astrocytes), low III tubulin (neural cells) and RECA-1 (endothelial cells) stainings were observed for non-treated lesions while GDNF-treated spinal cords presented less GFAP staining and more endothelial and nerve fiber infiltration in the lesion site. The animals treated with free GDNF-loaded hydrogel presented superior functional recovery compared with the animals treated with the GDNF microsphere-loaded hydrogels and non-treated animals.

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