The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea

[EN] Legumes usually have compound inflorescences, where flowers/pods develop from secondary inflorescences (I2), formed laterally at the primary inflorescence (I1). Number of flowers per I2, characteristic of each legume species, has important ecological and evolutionary relevance as it determines...

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Autores: Caballo, Cristina, Ortega, Raúl, Gil, Juan, Millán, Teresa, Rubio, Josefa, Berbel Tornero, Ana|||0000-0002-6446-0693, MADUEÑO, FRANCISCO|||0000-0001-7598-3003
Tipo de recurso: artículo
Fecha de publicación:2022
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/196985
Acceso en línea:https://riunet.upv.es/handle/10251/196985
Access Level:acceso abierto
Palabra clave:Chickpea breeding
Chickpea double-pod mutants
Inflorescence architecture
Meristem activity
R2R3-MYB genes, RAX
Genes
Seed yield
Compound inflorescence
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spelling The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpeaCaballo, CristinaOrtega, RaúlGil, JuanMillán, TeresaRubio, JosefaBerbel Tornero, Ana|||0000-0002-6446-0693MADUEÑO, FRANCISCO|||0000-0001-7598-3003Chickpea breedingChickpea double-pod mutantsInflorescence architectureMeristem activityR2R3-MYB genes, RAXGenesSeed yieldCompound inflorescence[EN] Legumes usually have compound inflorescences, where flowers/pods develop from secondary inflorescences (I2), formed laterally at the primary inflorescence (I1). Number of flowers per I2, characteristic of each legume species, has important ecological and evolutionary relevance as it determines diversity in inflorescence architecture; moreover, it is also agronomically important for its potential impact on yield. Nevertheless, the genetic network controlling the number of flowers per I2 is virtually unknown. Chickpea (Cicer arietinum) typically produces one flower per I2 but single flower (sfl) mutants produce two (double-pod phenotype). We isolated the SFL gene by mapping the sfl-d mutation and identifying and characterising a second mutant allele. We analysed the effect of sfl on chickpea inflorescence ontogeny with scanning electron microscopy and studied the expression of SFL and meristem identity genes by RNA in situ hybridisation. We show that SFL corresponds to CaRAX1/2a, which codes a MYB transcription factor specifically expressed in the I2 meristem. Our findings reveal SFL as a central factor controlling chickpea inflorescence architecture, acting in the I2 meristem to regulate the length of the period for which it remains active, and therefore determining the number of floral meristems that it can produce.We thank Ludovico Dreni for advice on phylogenetic analysis and M. J. Domenech for efficient technical assistance. We also thank C. Ferrandiz, M. A. Blazquez and J. L. Weller for helpful discussions and critical reading. Work at the FM laboratory was financed through grants from the Spanish Ministerio de Ciencia Innovacion y Universidades, FEDER and Generalitat Valenciana (BIO2015-64307-R, PGC2018-099232-B-I00 and Prometeo/2019/004). Work at IFAPA and UCO has been supported by INIA project RTA2017-00041-00-00, co-financed by the European Union through the ERDF2014-2020 'Programa Operativo de Crecimiento Inteligente'. CC acknowledges her PhD fellowship INIA-CCAA.Blackwell PublishingInstituto Universitario Mixto de Biología Molecular y Celular de PlantasGeneralitat ValencianaAgencia Estatal de InvestigaciónEuropean Regional Development FundMinisterio de Economía y CompetitividadInstituto Nacional de Investigaciones AgrariasRepositorio Institucional de la Universitat Politècnica de València Riunet20222022-05-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/196985reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-099232-B-I00 DESCIFRANDO EL MODO DE ACCION DE TERMINAL FLOWER 1Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2019%2F004Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2015-64307-R CONTROL GENETICO DE LA ARQUITECTURA DE LA INFLORESCENCIA DE LEGUMINOSAS: NUEVOS GENES PARA LA MEJORA DE SU RENDIMIENTOMinisterio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RTA2017-00041open accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial - Sin obra derivada (by-nc-nd) http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/1969852026-06-13T07:49:27Z
dc.title.none.fl_str_mv The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
title The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
spellingShingle The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
Caballo, Cristina
Chickpea breeding
Chickpea double-pod mutants
Inflorescence architecture
Meristem activity
R2R3-MYB genes, RAX
Genes
Seed yield
Compound inflorescence
title_short The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
title_full The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
title_fullStr The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
title_full_unstemmed The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
title_sort The SINGLE FLOWER (SFL) gene encodes a MYB transcription factor that regulates the number of flowers produced by the inflorescence of chickpea
dc.creator.none.fl_str_mv Caballo, Cristina
Ortega, Raúl
Gil, Juan
Millán, Teresa
Rubio, Josefa
Berbel Tornero, Ana|||0000-0002-6446-0693
MADUEÑO, FRANCISCO|||0000-0001-7598-3003
author Caballo, Cristina
author_facet Caballo, Cristina
Ortega, Raúl
Gil, Juan
Millán, Teresa
Rubio, Josefa
Berbel Tornero, Ana|||0000-0002-6446-0693
MADUEÑO, FRANCISCO|||0000-0001-7598-3003
author_role author
author2 Ortega, Raúl
Gil, Juan
Millán, Teresa
Rubio, Josefa
Berbel Tornero, Ana|||0000-0002-6446-0693
MADUEÑO, FRANCISCO|||0000-0001-7598-3003
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Instituto Universitario Mixto de Biología Molecular y Celular de Plantas
Generalitat Valenciana
Agencia Estatal de Investigación
European Regional Development Fund
Ministerio de Economía y Competitividad
Instituto Nacional de Investigaciones Agrarias
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Chickpea breeding
Chickpea double-pod mutants
Inflorescence architecture
Meristem activity
R2R3-MYB genes, RAX
Genes
Seed yield
Compound inflorescence
topic Chickpea breeding
Chickpea double-pod mutants
Inflorescence architecture
Meristem activity
R2R3-MYB genes, RAX
Genes
Seed yield
Compound inflorescence
description [EN] Legumes usually have compound inflorescences, where flowers/pods develop from secondary inflorescences (I2), formed laterally at the primary inflorescence (I1). Number of flowers per I2, characteristic of each legume species, has important ecological and evolutionary relevance as it determines diversity in inflorescence architecture; moreover, it is also agronomically important for its potential impact on yield. Nevertheless, the genetic network controlling the number of flowers per I2 is virtually unknown. Chickpea (Cicer arietinum) typically produces one flower per I2 but single flower (sfl) mutants produce two (double-pod phenotype). We isolated the SFL gene by mapping the sfl-d mutation and identifying and characterising a second mutant allele. We analysed the effect of sfl on chickpea inflorescence ontogeny with scanning electron microscopy and studied the expression of SFL and meristem identity genes by RNA in situ hybridisation. We show that SFL corresponds to CaRAX1/2a, which codes a MYB transcription factor specifically expressed in the I2 meristem. Our findings reveal SFL as a central factor controlling chickpea inflorescence architecture, acting in the I2 meristem to regulate the length of the period for which it remains active, and therefore determining the number of floral meristems that it can produce.
publishDate 2022
dc.date.none.fl_str_mv 2022
2022-05-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/196985
url https://riunet.upv.es/handle/10251/196985
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020 PGC2018-099232-B-I00 DESCIFRANDO EL MODO DE ACCION DE TERMINAL FLOWER 1
Generalitat Valenciana https://doi.org/10.13039/501100003359 PROMETEO%2F2019%2F004
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 BIO2015-64307-R CONTROL GENETICO DE LA ARQUITECTURA DE LA INFLORESCENCIA DE LEGUMINOSAS: NUEVOS GENES PARA LA MEJORA DE SU RENDIMIENTO
Ministerio de Economía y Competitividad http://dx.doi.org/10.13039/501100003329 RTA2017-00041
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial - Sin obra derivada (by-nc-nd)
http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Blackwell Publishing
publisher.none.fl_str_mv Blackwell Publishing
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300719

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