Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake

Scope: Absorption mechanisms of phytoene (PT) and phytofluene (PTF) are poorly known. The main objectives of the study are to measure their micellization and intestinal cell uptake efficiencies and to compare them to those of commonly consumed carotenoids. Other objectives are to assess the involvem...

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Autores: Mapelli Brahm, Paula, Desmarchelier, Charles, Margier, Marielle, Reboul, Emmanuelle, Meléndez Martínez, Antonio Jesús, Borel, Patrick
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2018
País:España
Institución:Universidad de Sevilla (US)
Repositorio:idUS. Depósito de Investigación de la Universidad de Sevilla
OAI Identifier:oai:idus.us.es:11441/137062
Acceso en línea:https://hdl.handle.net/11441/137062
https://doi.org/10.1002/mnfr.201800703
Access Level:acceso abierto
Palabra clave:Bioaccessibility
Bioavailability
Carotenoids
Lutein
β-carotene
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spelling Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular UptakeMapelli Brahm, PaulaDesmarchelier, CharlesMargier, MarielleReboul, EmmanuelleMeléndez Martínez, Antonio JesúsBorel, PatrickBioaccessibilityBioavailabilityCarotenoidsLuteinβ-caroteneScope: Absorption mechanisms of phytoene (PT) and phytofluene (PTF) are poorly known. The main objectives of the study are to measure their micellization and intestinal cell uptake efficiencies and to compare them to those of commonly consumed carotenoids. Other objectives are to assess the involvement of protein(s) in their cellular uptake and whether they compete with other carotenoids for micellization and cellular uptake. Methods and results: Tomato-extract-purified PT and PTF, mainly present as cis-isomers, are much better incorporated in synthetic mixed micelles than pure all-trans lycopene. PT impairs lycopene micellization (−56%, P < 0.05) while PT and PTF do not significantly affect the micellization of other carotenoids, and vice versa. At low concentration, Caco-2 PTF uptake is higher (P < 0.05) than that of PT and lycopene (29%, 21%, and not detectable). SR-BI, but not CD36 neither NPC1L1, is involved in PT and PTF uptake. PT and PTF impair (p < 0.05) β-carotene uptake (−13 and −22%, respectively). Conclusions: The high bioaccessibility of PT and PTF can be partly explained by their high micellization efficiency, which is likely due to their natural cis isomerization and/or to their high molecular flexibility. SR-BI is involved in their cellular uptake, which can explain competitions with other carotenoids.Junta de Andalucía CAROTINCO-P12-AGR-1287Premio Mensual Publicación Científica Destacada de la US. Facultad de FarmaciaWiley-BlackwellNutrición y Bromatología, Toxicología y Medicina LegalJunta de Andalucía2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://hdl.handle.net/11441/137062https://doi.org/10.1002/mnfr.201800703reponame:idUS. Depósito de Investigación de la Universidad de Sevillainstname:Universidad de Sevilla (US)InglésMolecular Nutrition and Food Research, 62 (22), 1800703.CAROTINCO-P12-AGR-1287https://doi.org/10.1002/mnfr.201800703info:eu-repo/semantics/openAccessoai:idus.us.es:11441/1370622026-06-17T12:51:07Z
dc.title.none.fl_str_mv Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
title Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
spellingShingle Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
Mapelli Brahm, Paula
Bioaccessibility
Bioavailability
Carotenoids
Lutein
β-carotene
title_short Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
title_full Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
title_fullStr Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
title_full_unstemmed Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
title_sort Phytoene and Phytofluene Isolated from a Tomato Extract are Readily Incorporated in Mixed Micelles and Absorbed by Caco-2 Cells, as Compared to Lycopene, and SR-BI is Involved in their Cellular Uptake
dc.creator.none.fl_str_mv Mapelli Brahm, Paula
Desmarchelier, Charles
Margier, Marielle
Reboul, Emmanuelle
Meléndez Martínez, Antonio Jesús
Borel, Patrick
author Mapelli Brahm, Paula
author_facet Mapelli Brahm, Paula
Desmarchelier, Charles
Margier, Marielle
Reboul, Emmanuelle
Meléndez Martínez, Antonio Jesús
Borel, Patrick
author_role author
author2 Desmarchelier, Charles
Margier, Marielle
Reboul, Emmanuelle
Meléndez Martínez, Antonio Jesús
Borel, Patrick
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Nutrición y Bromatología, Toxicología y Medicina Legal
Junta de Andalucía
dc.subject.none.fl_str_mv Bioaccessibility
Bioavailability
Carotenoids
Lutein
β-carotene
topic Bioaccessibility
Bioavailability
Carotenoids
Lutein
β-carotene
description Scope: Absorption mechanisms of phytoene (PT) and phytofluene (PTF) are poorly known. The main objectives of the study are to measure their micellization and intestinal cell uptake efficiencies and to compare them to those of commonly consumed carotenoids. Other objectives are to assess the involvement of protein(s) in their cellular uptake and whether they compete with other carotenoids for micellization and cellular uptake. Methods and results: Tomato-extract-purified PT and PTF, mainly present as cis-isomers, are much better incorporated in synthetic mixed micelles than pure all-trans lycopene. PT impairs lycopene micellization (−56%, P < 0.05) while PT and PTF do not significantly affect the micellization of other carotenoids, and vice versa. At low concentration, Caco-2 PTF uptake is higher (P < 0.05) than that of PT and lycopene (29%, 21%, and not detectable). SR-BI, but not CD36 neither NPC1L1, is involved in PT and PTF uptake. PT and PTF impair (p < 0.05) β-carotene uptake (−13 and −22%, respectively). Conclusions: The high bioaccessibility of PT and PTF can be partly explained by their high micellization efficiency, which is likely due to their natural cis isomerization and/or to their high molecular flexibility. SR-BI is involved in their cellular uptake, which can explain competitions with other carotenoids.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/11441/137062
https://doi.org/10.1002/mnfr.201800703
url https://hdl.handle.net/11441/137062
https://doi.org/10.1002/mnfr.201800703
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Molecular Nutrition and Food Research, 62 (22), 1800703.
CAROTINCO-P12-AGR-1287
https://doi.org/10.1002/mnfr.201800703
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv Wiley-Blackwell
publisher.none.fl_str_mv Wiley-Blackwell
dc.source.none.fl_str_mv reponame:idUS. Depósito de Investigación de la Universidad de Sevilla
instname:Universidad de Sevilla (US)
instname_str Universidad de Sevilla (US)
reponame_str idUS. Depósito de Investigación de la Universidad de Sevilla
collection idUS. Depósito de Investigación de la Universidad de Sevilla
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,300724

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