Transmission of sheep-bovine spongiform encephalopathy to pigs

Experimental transmission of the bovine spongiform encephalopathy (BSE) agent has been successfully reported in pigs inoculated via three simultaneous distinct routes (intracerebral, intraperitoneal and intravenous). Sheep derived BSE (Sh-BSE) is transmitted more efficiently than the original cattle...

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Autores: Hedman, C., Bolea, R., Marín, B., Cobrière, F., Filali, H., Vazquez, F., Pitarch, J.L., Vargas, A., Acín, C., Moreno, B., Pumarola, M., Andreoletti, O., Badiola, J.J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Institución:Universidad de Zaragoza
Repositorio:Zaguán. Repositorio Digital de la Universidad de Zaragoza
OAI Identifier:oai:zaguan.unizar.es:47389
Acceso en línea:http://zaguan.unizar.es/record/47389
Access Level:acceso abierto
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spelling Transmission of sheep-bovine spongiform encephalopathy to pigsHedman, C.Bolea, R.Marín, B.Cobrière, F.Filali, H.Vazquez, F.Pitarch, J.L.Vargas, A.Acín, C.Moreno, B.Pumarola, M.Andreoletti, O.Badiola, J.J.Experimental transmission of the bovine spongiform encephalopathy (BSE) agent has been successfully reported in pigs inoculated via three simultaneous distinct routes (intracerebral, intraperitoneal and intravenous). Sheep derived BSE (Sh-BSE) is transmitted more efficiently than the original cattle-BSE isolate in a transgenic mouse model expressing porcine prion protein. However, the neuropathology and distribution of Sh-BSE in pigs as natural hosts, and susceptibility to this agent, is unknown. In the present study, seven pigs were intracerebrally inoculated with Sh-BSE prions. One pig was euthanized for analysis in the preclinical disease stage. The remaining six pigs developed neurological signs and histopathology revealed severe spongiform changes accompanied by astrogliosis and microgliosis throughout the central nervous system. Intracellular and neuropil-associated pathological prion protein (PrPSc) deposition was consistently observed in different brain sections and corroborated by Western blot. PrPSc was detected by immunohistochemistry and enzyme immunoassay in the following tissues in at least one animal: lymphoid tissues, peripheral nerves, gastrointestinal tract, skeletal muscle, adrenal gland and pancreas. PrPSc deposition was revealed by immunohistochemistry alone in the retina, optic nerve and kidney. These results demonstrate the efficient transmission of Sh-BSE in pigs and show for the first time that in this species propagation of bovine PrPSc in a wide range of peripheral tissues is possible. These results provide important insight into the distribution and detection of prions in non-ruminant animals.2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://zaguan.unizar.es/record/47389reponame:Zaguán. Repositorio Digital de la Universidad de Zaragozainstname:Universidad de ZaragozaInglésinfo:eu-repo/semantics/openAccessoai:zaguan.unizar.es:473892026-05-29T13:59:51Z
dc.title.none.fl_str_mv Transmission of sheep-bovine spongiform encephalopathy to pigs
title Transmission of sheep-bovine spongiform encephalopathy to pigs
spellingShingle Transmission of sheep-bovine spongiform encephalopathy to pigs
Hedman, C.
title_short Transmission of sheep-bovine spongiform encephalopathy to pigs
title_full Transmission of sheep-bovine spongiform encephalopathy to pigs
title_fullStr Transmission of sheep-bovine spongiform encephalopathy to pigs
title_full_unstemmed Transmission of sheep-bovine spongiform encephalopathy to pigs
title_sort Transmission of sheep-bovine spongiform encephalopathy to pigs
dc.creator.none.fl_str_mv Hedman, C.
Bolea, R.
Marín, B.
Cobrière, F.
Filali, H.
Vazquez, F.
Pitarch, J.L.
Vargas, A.
Acín, C.
Moreno, B.
Pumarola, M.
Andreoletti, O.
Badiola, J.J.
author Hedman, C.
author_facet Hedman, C.
Bolea, R.
Marín, B.
Cobrière, F.
Filali, H.
Vazquez, F.
Pitarch, J.L.
Vargas, A.
Acín, C.
Moreno, B.
Pumarola, M.
Andreoletti, O.
Badiola, J.J.
author_role author
author2 Bolea, R.
Marín, B.
Cobrière, F.
Filali, H.
Vazquez, F.
Pitarch, J.L.
Vargas, A.
Acín, C.
Moreno, B.
Pumarola, M.
Andreoletti, O.
Badiola, J.J.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
description Experimental transmission of the bovine spongiform encephalopathy (BSE) agent has been successfully reported in pigs inoculated via three simultaneous distinct routes (intracerebral, intraperitoneal and intravenous). Sheep derived BSE (Sh-BSE) is transmitted more efficiently than the original cattle-BSE isolate in a transgenic mouse model expressing porcine prion protein. However, the neuropathology and distribution of Sh-BSE in pigs as natural hosts, and susceptibility to this agent, is unknown. In the present study, seven pigs were intracerebrally inoculated with Sh-BSE prions. One pig was euthanized for analysis in the preclinical disease stage. The remaining six pigs developed neurological signs and histopathology revealed severe spongiform changes accompanied by astrogliosis and microgliosis throughout the central nervous system. Intracellular and neuropil-associated pathological prion protein (PrPSc) deposition was consistently observed in different brain sections and corroborated by Western blot. PrPSc was detected by immunohistochemistry and enzyme immunoassay in the following tissues in at least one animal: lymphoid tissues, peripheral nerves, gastrointestinal tract, skeletal muscle, adrenal gland and pancreas. PrPSc deposition was revealed by immunohistochemistry alone in the retina, optic nerve and kidney. These results demonstrate the efficient transmission of Sh-BSE in pigs and show for the first time that in this species propagation of bovine PrPSc in a wide range of peripheral tissues is possible. These results provide important insight into the distribution and detection of prions in non-ruminant animals.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.none.fl_str_mv http://zaguan.unizar.es/record/47389
url http://zaguan.unizar.es/record/47389
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv
publisher.none.fl_str_mv
dc.source.none.fl_str_mv reponame:Zaguán. Repositorio Digital de la Universidad de Zaragoza
instname:Universidad de Zaragoza
instname_str Universidad de Zaragoza
reponame_str Zaguán. Repositorio Digital de la Universidad de Zaragoza
collection Zaguán. Repositorio Digital de la Universidad de Zaragoza
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