Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir

5 Pág.

Detalles Bibliográficos
Autores: Ferreira e Silva, Joana, Jiménez-Ruiz, Saúl, Rodrigues, Marisa, Santos, Emídio, Castro-Scholten, Sabrina, Lizana, Vitor, Martí-Marco, Alba, Almeida, Tereza, Lopes, Ana Margarida, Abrantes, Joana, Bárcena, Juan, Blanco, Esther, Rouco, Carlos, García-Bocanegra, I., Alves, Paulo Célio, Santos, Nuno
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/372135
Acceso en línea:http://hdl.handle.net/10261/372135
https://api.elsevier.com/content/abstract/scopus_id/85208478545
Access Level:acceso abierto
Palabra clave:Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
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dc.title.none.fl_str_mv Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
title Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
spellingShingle Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
Ferreira e Silva, Joana
Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
title_short Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
title_full Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
title_fullStr Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
title_full_unstemmed Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
title_sort Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoir
dc.creator.none.fl_str_mv Ferreira e Silva, Joana
Jiménez-Ruiz, Saúl
Rodrigues, Marisa
Santos, Emídio
Castro-Scholten, Sabrina
Lizana, Vitor
Martí-Marco, Alba
Almeida, Tereza
Lopes, Ana Margarida
Abrantes, Joana
Bárcena, Juan
Blanco, Esther
Rouco, Carlos
García-Bocanegra, I.
Alves, Paulo Célio
Santos, Nuno
author Ferreira e Silva, Joana
author_facet Ferreira e Silva, Joana
Jiménez-Ruiz, Saúl
Rodrigues, Marisa
Santos, Emídio
Castro-Scholten, Sabrina
Lizana, Vitor
Martí-Marco, Alba
Almeida, Tereza
Lopes, Ana Margarida
Abrantes, Joana
Bárcena, Juan
Blanco, Esther
Rouco, Carlos
García-Bocanegra, I.
Alves, Paulo Célio
Santos, Nuno
author_role author
author2 Jiménez-Ruiz, Saúl
Rodrigues, Marisa
Santos, Emídio
Castro-Scholten, Sabrina
Lizana, Vitor
Martí-Marco, Alba
Almeida, Tereza
Lopes, Ana Margarida
Abrantes, Joana
Bárcena, Juan
Blanco, Esther
Rouco, Carlos
García-Bocanegra, I.
Alves, Paulo Célio
Santos, Nuno
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Fundação para a Ciência e a Tecnologia (Portugal)
European Commission
Ministerio de Ciencia, Innovación y Universidades (España)
Agencia Estatal de Investigación (España)
Ferreira e Silva, Joana [0000-0002-6515-0654]
Jiménez-Ruiz, Saúl [0000-0003-2090-9353]
Santos, Emídio [0000-0002-8055-8287]
Castro-Scholten, Sabrina [0000-0001-8761-5945]
Martí-Marco, Alba [0000-0003-4990-8338]
Lopes, Ana Margarida [0000-0002-7176-0969]
Abrantes, Joana [0000-0002-8391-7134]
Bárcena, Juan [0000-0001-6050-0654]
Blanco, Esther [0000-0001-8413-4222]
Rouco, Carlos [0000-0003-1026-3253]
García-Bocanegra, I. [0000-0003-3388-2604]
Alves, Paulo Célio [0000-0003-4797-0939]
Santos, Nuno [0000-0002-1676-107X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
topic Epidemiology
European rabbit
Oryctolagus cuniculus
Protein Saver cards
Wildlife
description 5 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/372135
https://api.elsevier.com/content/abstract/scopus_id/85208478545
url http://hdl.handle.net/10261/372135
https://api.elsevier.com/content/abstract/scopus_id/85208478545
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/AEI/PID2023–151954NB-100/
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111080RB-C21
https://doi.org/10.1016/j.prevetmed.2024.106369
Sí
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eu_rights_str_mv openAccess
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dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Evaluation of dried blood spots for serological surveys of myxoma and rabbit hemorrhagic disease viruses in their wild reservoirFerreira e Silva, JoanaJiménez-Ruiz, SaúlRodrigues, MarisaSantos, EmídioCastro-Scholten, SabrinaLizana, VitorMartí-Marco, AlbaAlmeida, TerezaLopes, Ana MargaridaAbrantes, JoanaBárcena, JuanBlanco, EstherRouco, CarlosGarcía-Bocanegra, I.Alves, Paulo CélioSantos, NunoEpidemiologyEuropean rabbitOryctolagus cuniculusProtein Saver cardsWildlife5 Pág.Myxoma (MYXV) and rabbit hemorrhagic disease (RHDV) viruses are pathogens of economic relevance for cuniculture and conservation concern for wild European rabbits (Oryctolagus cuniculus), recently classified as 'Endangered' in its native range. Large-scale serological surveys, facilitated by sample collection using dried blood spots (DBS), allow monitoring seroprevalence in the wild reservoir but require evaluating the technique for the host and pathogen of interest. This study aimed to evaluate Protein Saver 903 DBS for MYXV and RHDV (genotype GI.2) serological surveys in European rabbits. Paired serum and DBS collected from 172 rabbits harvested or found dead in the Iberian Peninsula were tested for IgG antibodies specific against MYXV and RHDV GI.2 using indirect ELISA. We found an almost perfect agreement between serum and DBS for MYXV (Cohen's κ=0.914, CI95 0.847 - 0.981) and a strong agreement for RHDV GI.2 (Cohen's κ=0.808, CI95=0.722 - 0.893). The diagnostic sensitivity of DBS was 95.4 % (CI95 90.3 - 97.9 %) for MYXV and 82.1 % (CI95 73.2 - 88.5 %) for RHDV GI.2. The diagnostic specificity and positive predictive value were 100 % for both pathogens. This study supports DBS as a suitable sampling strategy for serological surveys of antibodies specific to MYXV and RHDV GI.2 in European rabbits, which generally agrees with results from other hosts and pathogens where this technique was evaluated.This work was supported by Fundação para a Ciência e Tecnologia (FCT) [grant SFRH/BPD/116596/2016 to N.S., CEECIND/01388/2017 to A.M.L. and CEECIND/00078/2017 to J.A.] and co-funded by the project NORTE-01–0246-FEDER-000063, supported by Norte Portugal Regional Operational Programme (NORTE2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). This study was partially funded by project LAGMED (www.lagmed.eu), supported by FCT (PRIMA/0003/2018), the Spanish Ministry of Science, Innovation and University (PRIMA S2–11-PCI2019–103732 to E.B.) and PRIMA programme, an Art. 185 initiative supported and funded under Horizon 2020, the European Union’s Framework Programme for Research and Innovation. This work benefited from research grants funded by the Spanish Ministry of Science and Innovation (projects Iber-Lagohealth; PID2023–151954NB-100 and LagoHealth; PID2019–111080RB-C21). It was also partially funded by the Sub-modality 2.4. "UCOLIDERA" of the "Enrique Aguilar Benítez de Lugo" Research Plan of the University of Cordoba and CIBER -Consorcio Centro de Investigación Biomédica en Red- (CB 2021), Instituto de Salud Carlos III, Ministerio de Ciencia e Innovación and European Union – NextGenerationEU. S.J.R. is supported by a ‘Juan de la Cierva’ contract (JDC2022–048850-I) funded by the MCIN/AEI/10.13039/501100011033 and by the European Union “NextGenerationEU”/PRTR. S.C.S. is supported by an FPU grant from the Spanish Ministry of Universities (FPU19/06026). This work was supported by Life Iberconejo (LIFE20-GIE ES000731).Peer reviewedElsevierFundação para a Ciência e a Tecnologia (Portugal)European CommissionMinisterio de Ciencia, Innovación y Universidades (España)Agencia Estatal de Investigación (España)Ferreira e Silva, Joana [0000-0002-6515-0654]Jiménez-Ruiz, Saúl [0000-0003-2090-9353]Santos, Emídio [0000-0002-8055-8287]Castro-Scholten, Sabrina [0000-0001-8761-5945]Martí-Marco, Alba [0000-0003-4990-8338]Lopes, Ana Margarida [0000-0002-7176-0969]Abrantes, Joana [0000-0002-8391-7134]Bárcena, Juan [0000-0001-6050-0654]Blanco, Esther [0000-0001-8413-4222]Rouco, Carlos [0000-0003-1026-3253]García-Bocanegra, I. [0000-0003-3388-2604]Alves, Paulo Célio [0000-0003-4797-0939]Santos, Nuno [0000-0002-1676-107X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/372135https://api.elsevier.com/content/abstract/scopus_id/85208478545reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PCI2019-103732info:eu-repo/grantAgreement/AEI/PID2023–151954NB-100/info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111080RB-C21https://doi.org/10.1016/j.prevetmed.2024.106369Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3721352026-05-22T06:33:51Z
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