Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study

Purpose The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. Methods This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a Univ...

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Detalles Bibliográficos
Autores: Rocafort, Muntsa, Henares, Desiree, Brotons, Pedro, Barrabeig i Fabregat, Irene, Launes Montaña, Cristian, Merdrignac, Lore, Valenciano, Marta, Domínguez García, Àngela, Godoy, Pere, Muñoz-Almagro, Carmen
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/194964
Acceso en línea:https://hdl.handle.net/2445/194964
Access Level:acceso abierto
Palabra clave:Nodrissons
Microbiota
Tos ferina
Rinofaringe
Infants
Whooping cough
Nasopharynx
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spelling Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control studyRocafort, MuntsaHenares, DesireeBrotons, PedroBarrabeig i Fabregat, IreneLaunes Montaña, CristianMerdrignac, LoreValenciano, MartaDomínguez García, ÀngelaGodoy, PereMuñoz-Almagro, CarmenNodrissonsMicrobiotaTos ferinaRinofaringeInfantsMicrobiotaWhooping coughNasopharynxPurpose The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. Methods This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. Results Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy controPublic Library of Science (PLoS)2023202320212023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion16 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/194964Articles publicats en revistes (Medicina)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1371/journal.pone.0259318PLoS One, 2021, vol. 16, num. 10, p. e0259318https://doi.org/10.1371/journal.pone.0259318cc-by (c) Rocafort, Muntsa et al., 2021https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1949642026-05-29T05:05:01Z
dc.title.none.fl_str_mv Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
title Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
spellingShingle Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
Rocafort, Muntsa
Nodrissons
Microbiota
Tos ferina
Rinofaringe
Infants
Microbiota
Whooping cough
Nasopharynx
title_short Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
title_full Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
title_fullStr Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
title_full_unstemmed Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
title_sort Exploring the nasopharyngeal microbiota composition in infants with whooping cough: a test-negative case-control study
dc.creator.none.fl_str_mv Rocafort, Muntsa
Henares, Desiree
Brotons, Pedro
Barrabeig i Fabregat, Irene
Launes Montaña, Cristian
Merdrignac, Lore
Valenciano, Marta
Domínguez García, Àngela
Godoy, Pere
Muñoz-Almagro, Carmen
author Rocafort, Muntsa
author_facet Rocafort, Muntsa
Henares, Desiree
Brotons, Pedro
Barrabeig i Fabregat, Irene
Launes Montaña, Cristian
Merdrignac, Lore
Valenciano, Marta
Domínguez García, Àngela
Godoy, Pere
Muñoz-Almagro, Carmen
author_role author
author2 Henares, Desiree
Brotons, Pedro
Barrabeig i Fabregat, Irene
Launes Montaña, Cristian
Merdrignac, Lore
Valenciano, Marta
Domínguez García, Àngela
Godoy, Pere
Muñoz-Almagro, Carmen
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Nodrissons
Microbiota
Tos ferina
Rinofaringe
Infants
Microbiota
Whooping cough
Nasopharynx
topic Nodrissons
Microbiota
Tos ferina
Rinofaringe
Infants
Microbiota
Whooping cough
Nasopharynx
description Purpose The purpose of this study was to characterize the nasopharyngeal microbiota of infants with possible and confirmed pertussis compared to healthy controls. Methods This prospective study included all infants <1 year with microbiologically confirmed diagnosis of pertussis attended at a University Hospital over a 12-month period. For each confirmed case, up to 2 consecutive patients within the same age range and meeting the clinical case definition of pertussis but testing PCR-negative were included as possible cases. A third group of asymptomatic infants (healthy controls) were also included. Nasopharyngeal microbiota was characterized by sequencing the V3-V4 region of the 16S rRNA gene. Common respiratory DNA/RNA viral co-infection was tested by multiplex PCR. Results Twelve confirmed cases, 21 possible cases and 9 healthy controls were included. Confirmed whooping cough was primarily driven by detection of Bordetella with no other major changes on nasopharyngeal microbiota. Possible cases had limited abundance or absence of Bordetella and a distinctive microbiota with lower bacterial richness and diversity and higher rates of viral co-infection than both confirmed cases and healthy controls. Bordetella reads determined by 16S rRNA gene sequencing were found in all 12 confirmed cases (100%), 3 out of the 21 possible cases (14.3%) but in any healthy contro
publishDate 2021
dc.date.none.fl_str_mv 2021
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/194964
url https://hdl.handle.net/2445/194964
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1371/journal.pone.0259318
PLoS One, 2021, vol. 16, num. 10, p. e0259318
https://doi.org/10.1371/journal.pone.0259318
dc.rights.none.fl_str_mv cc-by (c) Rocafort, Muntsa et al., 2021
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Rocafort, Muntsa et al., 2021
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 16 p.
application/pdf
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (Medicina)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.811543

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