Differentiation of blood and environmental interfering stains on substrates by Chemometrics-Assisted ATR FTIR spectroscopy

Blood is the most common and relevant bodily fluid that can be found in crime scenes. It is critical to correctly identify it, and to be able to differentiate it from other substances that may also appear at the crime scene. In this work, several stains of blood, chocolate, ketchup, and tomato sauce...

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Detalles Bibliográficos
Autores: Cano Trujillo, Cristina Pilar|||0000-0002-9587-4514, García Ruiz, Carmen|||0000-0001-5925-3449, Montalvo García, Gemma|||0000-0002-5640-8908, Ortega Ojeda, Fernando Ernesto
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universidad de Alcalá (UAH)
Repositorio:e_Buah Biblioteca Digital Universidad de Alcalá
Idioma:inglés
OAI Identifier:oai:ebuah.uah.es:10017/55795
Acceso en línea:http://hdl.handle.net/10017/55795
https://dx.doi.org/10.1016/j.saa.2023.122409
Access Level:acceso abierto
Palabra clave:Blood
Interferent
Substrate
Vibrational spectroscopy
ATR FTIR
OPLS-DA
Química
Chemistry
Descripción
Sumario:Blood is the most common and relevant bodily fluid that can be found in crime scenes. It is critical to correctly identify it, and to be able to differentiate it from other substances that may also appear at the crime scene. In this work, several stains of blood, chocolate, ketchup, and tomato sauce on five different substrates (plywood, metal, gauze, denim, and glass) were analysed by ATR FTIR spectroscopy assisted with orthogonal partial least square discriminant analysis (OPLS-DA) models. It was possible to differentiate blood from the environmental interfering substances independently of the substrate they were on, and to differentiate bloodstains according to the substrate they were deposited on. These results represent a proof-of-concept that open new horizons to differentiate bloodstains from other interfering substances on common substrates present in crime scenes.