Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation

Aquaporins are a family of ubiquitous transmembrane proteins that allow the transport of water and small molecules across the cell plasma membrane. The different members of this family present a characteristic distribution across different cell types, which is species-specific. In mammalian sperm, d...

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Autores: Delgado Bermúdez, Ariadna, Recuero, Sandra, Llavanera, Marc, Mateo Otero, Yentel, Sandu, Andra, Barranco, Isabel, Ribas Maynou, Jordi, Yeste Oliveras, Marc
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10256/19849
Acceso en línea:http://hdl.handle.net/10256/19849
Access Level:acceso abierto
Palabra clave:Aquaporins
Aquaporines
Espermatozoides -- Investigació
Spermatozoa -- Research
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oai_identifier_str oai:recercat.cat:10256/19849
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network_name_str España
repository_id_str
spelling Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitationDelgado Bermúdez, AriadnaRecuero, SandraLlavanera, MarcMateo Otero, YentelSandu, AndraBarranco, IsabelRibas Maynou, JordiYeste Oliveras, MarcAquaporinsAquaporinesEspermatozoides -- InvestigacióSpermatozoa -- ResearchAquaporins are a family of ubiquitous transmembrane proteins that allow the transport of water and small molecules across the cell plasma membrane. The different members of this family present a characteristic distribution across different cell types, which is species-specific. In mammalian sperm, different AQPs, including AQP3, AQP7 and AQP11, have been identified; their main roles are related to osmoadaptation and sperm motility activation after ejaculation. Capacitation, which is a post-ejaculatory process that sperm must undergo to achieve fertilizing ability, is triggered by pH changes and different extracellular ions that are present in the female reproductive tract. Considering the function of AQPs and their influence on pH through the regulation of water flow, this study aimed to elucidate the potential role of different AQPs during in vitro sperm capacitation using three different transition metal compounds as AQP inhibitors. Cooper sulphate, a specific inhibitor of AQP3, caused a drastic increase in peroxide intracellular levels compared to the control. Mercury chloride, an unspecific inhibitor of all AQPs except AQP7 produced an increase in membrane lipid disorder and led to a decrease in sperm motility and kinetics parameters. Finally, the addition of silver sulfadiazine, an unspecific inhibitor of all AQPs, generated the same effects than mercury chloride, decreased the intracellular pH and altered tyrosine phosphorylation levels after the induction of the acrosome reaction. In the light of the aforementioned, a) the permeability of AQP3 to peroxides does not seem to be crucial for sperm capacitation and acrosome reaction; b) AQPs have a key role in preserving sperm motility during that process; and c) AQPs as a whole seem to contribute to the maintenance of lipid membrane architecture during capacitation and may be related to the intracellular signaling pathways involved in the acrosome reaction. Hence, further research aimed to elucidate the mechanisms underlying the involvement of AQPs in mammalian sperm capacitation and acrosome reaction is warrantedThis research was funded by the Ministry of Science and Innovation (Spain) (RYC-2014-15581, AGL2017-88329-R, and PRE2018-083488); and Regional Government of Catalonia, Spain (2017-SGR-1229)Frontiers MediaAgencia Estatal de InvestigaciónMinisterio de Economía y Competitividad (Espanya)2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer-reviewedapplication/pdfhttp://hdl.handle.net/10256/19849http://hdl.handle.net/10256/19849Frontiers in Cell and Developmental Biology, 2021, vol. 9, art.núm. 656438Articles publicats (D-B)Delgado Bermúdez, Ariadna Recuero, Sandra Llavanera, Marc Mateo Otero, Yentel Sandu, Andra Barranco, Isabel Ribas Maynou, Jordi Yeste Oliveras, Marc 2021 Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation Frontiers in Cell and Developmental Biology 9 art.núm. 656438reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)Inglésinfo:eu-repo/semantics/altIdentifier/doi/10.3389/fcell.2021.656438info:eu-repo/semantics/altIdentifier/eissn/2296-634Xinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-Rinfo:eu-repo/grantAgreement/MINECO//RYC-2014-15581Attribution 4.0 International (CC BY 4.0)https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10256/198492026-05-29T05:05:01Z
dc.title.none.fl_str_mv Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
title Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
spellingShingle Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
Delgado Bermúdez, Ariadna
Aquaporins
Aquaporines
Espermatozoides -- Investigació
Spermatozoa -- Research
title_short Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
title_full Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
title_fullStr Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
title_full_unstemmed Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
title_sort Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation
dc.creator.none.fl_str_mv Delgado Bermúdez, Ariadna
Recuero, Sandra
Llavanera, Marc
Mateo Otero, Yentel
Sandu, Andra
Barranco, Isabel
Ribas Maynou, Jordi
Yeste Oliveras, Marc
author Delgado Bermúdez, Ariadna
author_facet Delgado Bermúdez, Ariadna
Recuero, Sandra
Llavanera, Marc
Mateo Otero, Yentel
Sandu, Andra
Barranco, Isabel
Ribas Maynou, Jordi
Yeste Oliveras, Marc
author_role author
author2 Recuero, Sandra
Llavanera, Marc
Mateo Otero, Yentel
Sandu, Andra
Barranco, Isabel
Ribas Maynou, Jordi
Yeste Oliveras, Marc
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación
Ministerio de Economía y Competitividad (Espanya)
dc.subject.none.fl_str_mv Aquaporins
Aquaporines
Espermatozoides -- Investigació
Spermatozoa -- Research
topic Aquaporins
Aquaporines
Espermatozoides -- Investigació
Spermatozoa -- Research
description Aquaporins are a family of ubiquitous transmembrane proteins that allow the transport of water and small molecules across the cell plasma membrane. The different members of this family present a characteristic distribution across different cell types, which is species-specific. In mammalian sperm, different AQPs, including AQP3, AQP7 and AQP11, have been identified; their main roles are related to osmoadaptation and sperm motility activation after ejaculation. Capacitation, which is a post-ejaculatory process that sperm must undergo to achieve fertilizing ability, is triggered by pH changes and different extracellular ions that are present in the female reproductive tract. Considering the function of AQPs and their influence on pH through the regulation of water flow, this study aimed to elucidate the potential role of different AQPs during in vitro sperm capacitation using three different transition metal compounds as AQP inhibitors. Cooper sulphate, a specific inhibitor of AQP3, caused a drastic increase in peroxide intracellular levels compared to the control. Mercury chloride, an unspecific inhibitor of all AQPs except AQP7 produced an increase in membrane lipid disorder and led to a decrease in sperm motility and kinetics parameters. Finally, the addition of silver sulfadiazine, an unspecific inhibitor of all AQPs, generated the same effects than mercury chloride, decreased the intracellular pH and altered tyrosine phosphorylation levels after the induction of the acrosome reaction. In the light of the aforementioned, a) the permeability of AQP3 to peroxides does not seem to be crucial for sperm capacitation and acrosome reaction; b) AQPs have a key role in preserving sperm motility during that process; and c) AQPs as a whole seem to contribute to the maintenance of lipid membrane architecture during capacitation and may be related to the intracellular signaling pathways involved in the acrosome reaction. Hence, further research aimed to elucidate the mechanisms underlying the involvement of AQPs in mammalian sperm capacitation and acrosome reaction is warranted
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer-reviewed
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10256/19849
http://hdl.handle.net/10256/19849
url http://hdl.handle.net/10256/19849
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/semantics/altIdentifier/doi/10.3389/fcell.2021.656438
info:eu-repo/semantics/altIdentifier/eissn/2296-634X
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88329-R
info:eu-repo/grantAgreement/MINECO//RYC-2014-15581
dc.rights.none.fl_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Attribution 4.0 International (CC BY 4.0)
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv Frontiers in Cell and Developmental Biology, 2021, vol. 9, art.núm. 656438
Articles publicats (D-B)
Delgado Bermúdez, Ariadna Recuero, Sandra Llavanera, Marc Mateo Otero, Yentel Sandu, Andra Barranco, Isabel Ribas Maynou, Jordi Yeste Oliveras, Marc 2021 Aquaporins are essential to maintain motility and membrane lipid architecture during mammalian sperm capacitation Frontiers in Cell and Developmental Biology 9 art.núm. 656438
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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