QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase

Strawberry (Fragaria × ananassa) fruits are an excellent source of L-ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of...

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Autores: Muñoz, Pilar, Castillejo, Cristina, Gómez, José Antonio, Miranda, Luis, Lesemann, Silke, Olbricht, Klaus, Petit, Aurélie, Chartier, Philippe, Haugeneder, Annika, Trinkl, Johanna, Mazzoni, Luca, Masny, Agnieszka, Zurawicz, Edward, Ziegler, Freya Maria Rosemarie, Usadel, Björn, Schwab, Wilfried, Denoyes, Béatrice, Mezzetti, Bruno, Osorio, Sonia, Sánchez-Sevilla, José F, Amaya, Iraida
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/371774
Acesso em linha:http://hdl.handle.net/10261/371774
https://api.elsevier.com/content/abstract/scopus_id/85159687598
Access Level:Acceso aberto
Palavra-chave:strawberry fruit
complex genetic
phosphorylase
QTL analysis
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spelling QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylaseMuñoz, PilarCastillejo, CristinaGómez, José AntonioMiranda, LuisLesemann, SilkeOlbricht, KlausPetit, AurélieChartier, PhilippeHaugeneder, AnnikaTrinkl, JohannaMazzoni, LucaMasny, AgnieszkaZurawicz, EdwardZiegler, Freya Maria RosemarieUsadel, BjörnSchwab, WilfriedDenoyes, BéatriceMezzetti, BrunoOsorio, SoniaSánchez-Sevilla, José FAmaya, Iraidastrawberry fruitcomplex geneticphosphorylaseQTL analysisStrawberry (Fragaria × ananassa) fruits are an excellent source of L-ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines 'Candonga' and 'Senga Sengana', adapted to distinct Southern and Northern European areas. To account for environmental effects, the F1 and parental lines were grown and phenotyped in five locations across Europe (France, Germany, Italy, Poland and Spain). Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold, with significant differences among genotypes and environments. AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus (QTL) analysis. Environmentally stable QTLs for AsA content were detected in linkage group (LG) 3A, LG 5A, LG 5B, LG 6B and LG 7C. Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A), and the chloroplast-located AsA transporter gene FaPHT4;4(7C) might be the underlying genetic factors for QTLs on LG 3A and 7C, respectively. We show that recessive alleles of FaGGP(3A) inherited from both parental lines increase fruit AsA content. Furthermore, expression of FaGGP(3A) was two-fold higher in lines with high AsA. Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.This research was supported by the European Union’s Horizon 2020 research and innovation program (GoodBerry; grant agreement number 679303), Agencia Estatal de Investigación (PID2019-111496RR-I00 / AEI / 10.13039/501100011033) and PR.AVA.AVA2019.034 (IFAPA, FEDER funds)Peer reviewedOxford University PressEuropean CommissionAgencia Estatal de Investigación (España)Instituto de Investigación y Formación Agraria y Pesquera (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/371774https://api.elsevier.com/content/abstract/scopus_id/85159687598reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/H2020/679303info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111496RR-I00Horticulture researchhttps://doi.org/10.1093/hr/uhad006Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3717742026-05-22T06:33:51Z
dc.title.none.fl_str_mv QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
title QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
spellingShingle QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
Muñoz, Pilar
strawberry fruit
complex genetic
phosphorylase
QTL analysis
title_short QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
title_full QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
title_fullStr QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
title_full_unstemmed QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
title_sort QTL analysis for ascorbic acid content in strawberry fruit reveals a complex genetic architecture and association with GDP-L-galactose phosphorylase
dc.creator.none.fl_str_mv Muñoz, Pilar
Castillejo, Cristina
Gómez, José Antonio
Miranda, Luis
Lesemann, Silke
Olbricht, Klaus
Petit, Aurélie
Chartier, Philippe
Haugeneder, Annika
Trinkl, Johanna
Mazzoni, Luca
Masny, Agnieszka
Zurawicz, Edward
Ziegler, Freya Maria Rosemarie
Usadel, Björn
Schwab, Wilfried
Denoyes, Béatrice
Mezzetti, Bruno
Osorio, Sonia
Sánchez-Sevilla, José F
Amaya, Iraida
author Muñoz, Pilar
author_facet Muñoz, Pilar
Castillejo, Cristina
Gómez, José Antonio
Miranda, Luis
Lesemann, Silke
Olbricht, Klaus
Petit, Aurélie
Chartier, Philippe
Haugeneder, Annika
Trinkl, Johanna
Mazzoni, Luca
Masny, Agnieszka
Zurawicz, Edward
Ziegler, Freya Maria Rosemarie
Usadel, Björn
Schwab, Wilfried
Denoyes, Béatrice
Mezzetti, Bruno
Osorio, Sonia
Sánchez-Sevilla, José F
Amaya, Iraida
author_role author
author2 Castillejo, Cristina
Gómez, José Antonio
Miranda, Luis
Lesemann, Silke
Olbricht, Klaus
Petit, Aurélie
Chartier, Philippe
Haugeneder, Annika
Trinkl, Johanna
Mazzoni, Luca
Masny, Agnieszka
Zurawicz, Edward
Ziegler, Freya Maria Rosemarie
Usadel, Björn
Schwab, Wilfried
Denoyes, Béatrice
Mezzetti, Bruno
Osorio, Sonia
Sánchez-Sevilla, José F
Amaya, Iraida
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación (España)
Instituto de Investigación y Formación Agraria y Pesquera (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv strawberry fruit
complex genetic
phosphorylase
QTL analysis
topic strawberry fruit
complex genetic
phosphorylase
QTL analysis
description Strawberry (Fragaria × ananassa) fruits are an excellent source of L-ascorbic acid (AsA), a powerful antioxidant for plants and humans. Identifying the genetic components underlying AsA accumulation is crucial for enhancing strawberry nutritional quality. Here, we unravel the genetic architecture of AsA accumulation using an F1 population derived from parental lines 'Candonga' and 'Senga Sengana', adapted to distinct Southern and Northern European areas. To account for environmental effects, the F1 and parental lines were grown and phenotyped in five locations across Europe (France, Germany, Italy, Poland and Spain). Fruit AsA content displayed normal distribution typical of quantitative traits and ranged five-fold, with significant differences among genotypes and environments. AsA content in each country and the average in all of them was used in combination with 6,974 markers for quantitative trait locus (QTL) analysis. Environmentally stable QTLs for AsA content were detected in linkage group (LG) 3A, LG 5A, LG 5B, LG 6B and LG 7C. Candidate genes were identified within stable QTL intervals and expression analysis in lines with contrasting AsA content suggested that GDP-L-Galactose Phosphorylase FaGGP(3A), and the chloroplast-located AsA transporter gene FaPHT4;4(7C) might be the underlying genetic factors for QTLs on LG 3A and 7C, respectively. We show that recessive alleles of FaGGP(3A) inherited from both parental lines increase fruit AsA content. Furthermore, expression of FaGGP(3A) was two-fold higher in lines with high AsA. Markers here identified represent a useful resource for efficient selection of new strawberry cultivars with increased AsA content.
publishDate 2023
dc.date.none.fl_str_mv 2023
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/371774
https://api.elsevier.com/content/abstract/scopus_id/85159687598
url http://hdl.handle.net/10261/371774
https://api.elsevier.com/content/abstract/scopus_id/85159687598
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/H2020/679303
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-111496RR-I00
Horticulture research
https://doi.org/10.1093/hr/uhad006
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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