Microbial characterisation and stability of a farmhouse natural fermented milk from Spain

This work reports the microbial characterisation of a farmhouse natural fermented milk (NFM) with good sensorial properties produced in Spain. Culturing and denaturing gradient gel electrophoresis (DGGE) analyses showed thatLactococcus lactissubsp.lactis and L. lactissubsp.cremoris(approximate level...

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Detalles Bibliográficos
Autores: Alegría, Ángel, Fernández, Elena, Delgado, Susana, Mayo Pérez, Baltasar
Tipo de recurso: artículo
Fecha de publicación:2010
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/51452
Acceso en línea:http://hdl.handle.net/10261/51452
Access Level:acceso abierto
Palabra clave:Natural fermented milk
Lactic acid bacteria
Traditional dairy products
Lactococcus lactis subsp. lactis
Lactococcus lactis subsp. cremoris
Lactobacillus plantarum
Descripción
Sumario:This work reports the microbial characterisation of a farmhouse natural fermented milk (NFM) with good sensorial properties produced in Spain. Culturing and denaturing gradient gel electrophoresis (DGGE) analyses showed thatLactococcus lactissubsp.lactis and L. lactissubsp.cremoris(approximate levels of 109 cfu/mL) were dominant in this NFM, whileLactobacillus plantarumappeared at a lower level (106-107 cfu/mL). Repetitive extragenic palindromic (REP)-PCR typing of the isolates identified single strains each ofLc. lactissubsp.lactis, Lc. lactissubsp.cremorisandLb. plantarum. These three strains formed a stable microbial association which has been maintained for at least some decades. © 2010 Society of Dairy Technology.