Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells

7 pages, 6 figures.

Detalhes bibliográficos
Autores: Latasa, María Jesús, Belandia, Borja, Pascual, Ángel
Tipo de documento: artigo
Data de publicação:1998
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/24136
Acesso em linha:http://hdl.handle.net/10261/24136
Access Level:Acceso aberto
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spelling Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cellsLatasa, María JesúsBelandia, BorjaPascual, Ángel7 pages, 6 figures.The β-amyloid protein (Aβ), the major component of the senile plaques found in Alzheimer brains, derives from a larger β-amyloid precursor protein (APP). Alternative splicing of the APP gene yields three major APP messenger RNAs (mRNAs), which, in turn, give rise to the APP770, APP751, and APP695 protein isoforms. In this study we examined the effects of thyroid hormone on APP expression in N2a-β neuroblastoma cells. T3 caused a significant increase in the APP770 mRNA band, in detriment of the APP695 mRNA, which was proportionately reduced. In agreement with these results, T3 markedly altered the relative ratio of intracellular APP isoforms, increasing the amount of APP770 and causing an equivalent reduction of the immature APP695 isoform. In accordance with these results, the soluble APP695-derived form was specifically reduced in the culture medium obtained from T3-treated cells. In contrast, the increase in intracellular APP770 was not followed by an enhanced release of soluble derivatives of this isoform. These results suggest that T3 regulates not only APP gene splicing, but also the processing and secretion of the APP peptides. According to our results, thyroid hormone might play a role in the development of Alzheimer’s disease by modulating the intracellular and extracellular contents of APP isoforms.This work was supported by grants from the Dirección General de Investigación Científica y Técnica (PB93–0135), Comisión Interministerial de Ciencia y Tecnología (SAF 97–0183), and Fondo de Investigación Sanitaria (94/0272). Recipient of a fellowship from the Departamento de Educación y Cultura del Gobierno de Navarra.Peer reviewedEndocrine Society201020101998info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501338439 bytesapplication/pdfhttp://hdl.handle.net/10261/24136reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1210/en.139.6.2692info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/241362026-05-22T06:33:51Z
dc.title.none.fl_str_mv Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
title Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
spellingShingle Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
Latasa, María Jesús
title_short Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
title_full Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
title_fullStr Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
title_full_unstemmed Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
title_sort Thyroid hormones regulate β-amyloid gene splicing and protein secretion in neuroblastoma cells
dc.creator.none.fl_str_mv Latasa, María Jesús
Belandia, Borja
Pascual, Ángel
author Latasa, María Jesús
author_facet Latasa, María Jesús
Belandia, Borja
Pascual, Ángel
author_role author
author2 Belandia, Borja
Pascual, Ángel
author2_role author
author
description 7 pages, 6 figures.
publishDate 1998
dc.date.none.fl_str_mv 1998
2010
2010
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/24136
url http://hdl.handle.net/10261/24136
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1210/en.139.6.2692
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 338439 bytes
application/pdf
dc.publisher.none.fl_str_mv Endocrine Society
publisher.none.fl_str_mv Endocrine Society
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
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repository.mail.fl_str_mv
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