P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus

This article belongs to the Special Issue Bacteriophage Therapy a Renaissance Weapon Recent Developments and Application, 2nd Edition

Detalles Bibliográficos
Autores: Antonio Broncano-Lavado, Antonio, Aguilera-Correa, John Jairo, Roquet-Banères, Françoise, Kremer, Laurent, Mediero Muñoz, Aránzazu, Seoane Blanco, Mateo, Van Raaij, Mark Johan, Pagán, Israel, Esteban Moreno, Jaime, García-Quintanilla, Meritxell
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglés
OAI Identifier:oai:dnet:biblosearchi::8e735e4a0f25fb37b33ba7cd6fcb0599
Acceso en línea:https://hdl.handle.net/10486/770760
https://dx.doi.org/10.3390/antibiotics14080801
Access Level:acceso abierto
Palabra clave:phage therapy
mycobacteriophage
Mycobacterium abscessus
biofilm
granuloma
antibiotic
Medicina
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spelling P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessusAntonio Broncano-Lavado, AntonioAguilera-Correa, John JairoRoquet-Banères, FrançoiseKremer, LaurentMediero Muñoz, AránzazuSeoane Blanco, MateoVan Raaij, Mark JohanPagán, IsraelEsteban Moreno, JaimeGarcía-Quintanilla, Meritxellphage therapymycobacteriophageMycobacterium abscessusbiofilmgranulomaantibioticMedicinaThis article belongs to the Special Issue Bacteriophage Therapy a Renaissance Weapon Recent Developments and Application, 2nd EditionBackground/Objectives: Mycobacterium abscessus is an opportunistic pathogen causing infections mainly in patients with immunosuppression and chronic pulmonary pathologies. Extended treatment periods are needed to tackle this pathogen, bacterial eradication is rare, and recurrence can take place with time. New alternative treatments are being investigated, such as bacteriophage therapy. This work describes the characterization of the mycobacteriophage P3MA, showing its ability to infect clinical and standard M. abscessus strains. Methods: Phylogenetic analysis, electron microscopy, growth curves, biofilm assays, checkerboard, and granuloma-like medium studies were performed. Results: P3MA inhibited the growth of clinical samples in both planktonic and biofilm states as well as in a granuloma-like model. The study of the interaction with antibiotics revealed that P3MA exhibited an antagonistic effect combined with clarithromycin, indifference with amikacin, and synergy with imipenem. Conclusions: All these results suggest that, after genetic engineering, P3MA could be a promising candidate for phage therapy in combination with imipenem, including lung infectionsThis research was funded by CIBERINFEC-CIBER de Enfermedades Infecciosas () and by Vaincre la Mucoviscidose (RF20230503223) and the Association Gregory Lemarchal. M.J.v.R. was supported by grant PID2021–125597NB-I00 financed by MCIN/AEI/10.13039/ 501100011033 and the European Union Next Generation EU/PRTR and FEDER. M.G.-Q. was supported by the Subprograma Miguel Servet from the Ministerio de Ciencia e Innovación of Spain (CP19/00104), Instituto de Salud Carlos III (Plan Estatal de I + D+i 2017–2020), and co-funded by the European Social Fund “Investing in your future”. I.P. was supported by Ministerio de Ciencia e Innovación (Proyectos de Transición Ecológica y Digital, TED2021-130793B-I00)MDPIDepartamento de Medicina Preventiva y Salud Pública y MicrobiologíaFacultad de MedicinaGobierno de EspañaInstituto de Salud Carlos III20252025-08-06research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10486/770760https://dx.doi.org/10.3390/antibiotics1408080140867995reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Attribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:dnet:biblosearchi::8e735e4a0f25fb37b33ba7cd6fcb05992026-06-23T12:46:27Z
dc.title.none.fl_str_mv P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
title P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
spellingShingle P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
Antonio Broncano-Lavado, Antonio
phage therapy
mycobacteriophage
Mycobacterium abscessus
biofilm
granuloma
antibiotic
Medicina
title_short P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
title_full P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
title_fullStr P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
title_full_unstemmed P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
title_sort P3MA: A Promising Mycobacteriophage Infecting Mycobacterium abscessus
dc.creator.none.fl_str_mv Antonio Broncano-Lavado, Antonio
Aguilera-Correa, John Jairo
Roquet-Banères, Françoise
Kremer, Laurent
Mediero Muñoz, Aránzazu
Seoane Blanco, Mateo
Van Raaij, Mark Johan
Pagán, Israel
Esteban Moreno, Jaime
García-Quintanilla, Meritxell
author Antonio Broncano-Lavado, Antonio
author_facet Antonio Broncano-Lavado, Antonio
Aguilera-Correa, John Jairo
Roquet-Banères, Françoise
Kremer, Laurent
Mediero Muñoz, Aránzazu
Seoane Blanco, Mateo
Van Raaij, Mark Johan
Pagán, Israel
Esteban Moreno, Jaime
García-Quintanilla, Meritxell
author_role author
author2 Aguilera-Correa, John Jairo
Roquet-Banères, Françoise
Kremer, Laurent
Mediero Muñoz, Aránzazu
Seoane Blanco, Mateo
Van Raaij, Mark Johan
Pagán, Israel
Esteban Moreno, Jaime
García-Quintanilla, Meritxell
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Medicina Preventiva y Salud Pública y Microbiología
Facultad de Medicina
Gobierno de España
Instituto de Salud Carlos III
dc.subject.none.fl_str_mv phage therapy
mycobacteriophage
Mycobacterium abscessus
biofilm
granuloma
antibiotic
Medicina
topic phage therapy
mycobacteriophage
Mycobacterium abscessus
biofilm
granuloma
antibiotic
Medicina
description This article belongs to the Special Issue Bacteriophage Therapy a Renaissance Weapon Recent Developments and Application, 2nd Edition
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-08-06
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10486/770760
https://dx.doi.org/10.3390/antibiotics14080801
40867995
url https://hdl.handle.net/10486/770760
https://dx.doi.org/10.3390/antibiotics14080801
identifier_str_mv 40867995
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Attribution 4.0 International
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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