Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization

Degradation of extracellular matrix, particularly interstitial collagen, promotes plaque instability and contributes to restenosis after vascular injury. We have explored the effects of vitamins C and E on the collagen content and metalloproteinase-1 (MMP-1) expression after angioplasty in hyperchol...

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Autores: Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726, Rodriguez, J.A. (José Antonio)|||/items/dfeeb791-bd5d-4472-bce8-b7f54cdf8f31, Arias, R. (R.)|||/items/fdf7cd5b-a7bd-4cab-b4fa-f169f3a83e6a, Belzunce-Saldise, M. (Miriam)|||/items/a90582e5-fdba-4fe6-8057-5a072cc3cc18, Nespereira, B. (Beatriz)|||/items/292b11c9-c1f3-454d-84c4-fe2b04bb275a, Perez-Ilzarbe, M. (Maitane)|||/items/e337ae1b-d63e-44a6-a819-2f3aa71900c6, Roncal-Mancho, C. (Carmen)|||/items/6c0dc409-f0aa-412b-9d75-436ba8a0a4ff, Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
Tipo de recurso: artículo
Fecha de publicación:2003
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/22270
Acceso en línea:https://hdl.handle.net/10171/22270
Access Level:acceso abierto
Palabra clave:Antioxidant vitamins
Hypercholesterolemia
Atherosclerosis
Collagen
Metalloproteinase-1
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spelling Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilizationOrbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726Rodriguez, J.A. (José Antonio)|||/items/dfeeb791-bd5d-4472-bce8-b7f54cdf8f31Arias, R. (R.)|||/items/fdf7cd5b-a7bd-4cab-b4fa-f169f3a83e6aBelzunce-Saldise, M. (Miriam)|||/items/a90582e5-fdba-4fe6-8057-5a072cc3cc18Nespereira, B. (Beatriz)|||/items/292b11c9-c1f3-454d-84c4-fe2b04bb275aPerez-Ilzarbe, M. (Maitane)|||/items/e337ae1b-d63e-44a6-a819-2f3aa71900c6Roncal-Mancho, C. (Carmen)|||/items/6c0dc409-f0aa-412b-9d75-436ba8a0a4ffPáramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96Antioxidant vitaminsHypercholesterolemiaAtherosclerosisCollagenMetalloproteinase-1Degradation of extracellular matrix, particularly interstitial collagen, promotes plaque instability and contributes to restenosis after vascular injury. We have explored the effects of vitamins C and E on the collagen content and metalloproteinase-1 (MMP-1) expression after angioplasty in hypercholesterolemic pigs. Iliac angioplasty was performed on 18 minipigs divided into three diet groups: a normal-cholesterol (NC), a high-cholesterol (HC) and a high-cholesterol plus vitamins C+E (HCV). Four weeks later, after sacrifice, the vascular collagen content and MMP-1 protein expression, along with the plasma caseinolytic activity and lipid peroxidation, were measured. MMP-1 was also determined in arterial rings stimulated with native low-density lipoproteins (LDL) isolated from experimental groups. Cholesterol-rich diet augmented plasma lipid peroxidation (P<0.05), reduced the collagen content and increased vascular MMP-1 expression after injury (P<0.05). Enhanced caseinolytic activity (identified as MMP-1) was also observed in HC plasma samples and in supernatants from arterial rings incubated with HC-LDL. Vitamins C and E markedly increased neointimal collagen content (P<0.01), reduced the hypercholesterolemia-induced changes in vascular MMP-1 (P<0.05) and diminished plasma and ex vivo caseinolytic activity. Vitamins C and E may help stabilize atherosclerotic plaque after angioplasty and favor vascular remodeling by increasing collagen content and reducing vascular MMP-1 expression in porcine hypercholesterolemia.ElsevierDadun. Depósito Académico Digital Universidad de Navarra20122012-05-2820032003-01-0120032003-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/22270reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/222702026-06-21T12:47:57Z
dc.title.none.fl_str_mv Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
title Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
spellingShingle Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
Antioxidant vitamins
Hypercholesterolemia
Atherosclerosis
Collagen
Metalloproteinase-1
title_short Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
title_full Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
title_fullStr Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
title_full_unstemmed Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
title_sort Antioxidant vitamins increase the collagen content and reduce MMP-1 in a porcine model of atherosclerosis: implications for plaque stabilization
dc.creator.none.fl_str_mv Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
Rodriguez, J.A. (José Antonio)|||/items/dfeeb791-bd5d-4472-bce8-b7f54cdf8f31
Arias, R. (R.)|||/items/fdf7cd5b-a7bd-4cab-b4fa-f169f3a83e6a
Belzunce-Saldise, M. (Miriam)|||/items/a90582e5-fdba-4fe6-8057-5a072cc3cc18
Nespereira, B. (Beatriz)|||/items/292b11c9-c1f3-454d-84c4-fe2b04bb275a
Perez-Ilzarbe, M. (Maitane)|||/items/e337ae1b-d63e-44a6-a819-2f3aa71900c6
Roncal-Mancho, C. (Carmen)|||/items/6c0dc409-f0aa-412b-9d75-436ba8a0a4ff
Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
author Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
author_facet Orbe-Lopategui, J. (Josune)|||/items/033ea7ef-1eff-418a-9fc4-1e2d3498d726
Rodriguez, J.A. (José Antonio)|||/items/dfeeb791-bd5d-4472-bce8-b7f54cdf8f31
Arias, R. (R.)|||/items/fdf7cd5b-a7bd-4cab-b4fa-f169f3a83e6a
Belzunce-Saldise, M. (Miriam)|||/items/a90582e5-fdba-4fe6-8057-5a072cc3cc18
Nespereira, B. (Beatriz)|||/items/292b11c9-c1f3-454d-84c4-fe2b04bb275a
Perez-Ilzarbe, M. (Maitane)|||/items/e337ae1b-d63e-44a6-a819-2f3aa71900c6
Roncal-Mancho, C. (Carmen)|||/items/6c0dc409-f0aa-412b-9d75-436ba8a0a4ff
Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
author_role author
author2 Rodriguez, J.A. (José Antonio)|||/items/dfeeb791-bd5d-4472-bce8-b7f54cdf8f31
Arias, R. (R.)|||/items/fdf7cd5b-a7bd-4cab-b4fa-f169f3a83e6a
Belzunce-Saldise, M. (Miriam)|||/items/a90582e5-fdba-4fe6-8057-5a072cc3cc18
Nespereira, B. (Beatriz)|||/items/292b11c9-c1f3-454d-84c4-fe2b04bb275a
Perez-Ilzarbe, M. (Maitane)|||/items/e337ae1b-d63e-44a6-a819-2f3aa71900c6
Roncal-Mancho, C. (Carmen)|||/items/6c0dc409-f0aa-412b-9d75-436ba8a0a4ff
Páramo-Fernández, J.A. (José Antonio)|||/items/8c56baa6-2a43-4836-b91b-4eed15be3c96
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Antioxidant vitamins
Hypercholesterolemia
Atherosclerosis
Collagen
Metalloproteinase-1
topic Antioxidant vitamins
Hypercholesterolemia
Atherosclerosis
Collagen
Metalloproteinase-1
description Degradation of extracellular matrix, particularly interstitial collagen, promotes plaque instability and contributes to restenosis after vascular injury. We have explored the effects of vitamins C and E on the collagen content and metalloproteinase-1 (MMP-1) expression after angioplasty in hypercholesterolemic pigs. Iliac angioplasty was performed on 18 minipigs divided into three diet groups: a normal-cholesterol (NC), a high-cholesterol (HC) and a high-cholesterol plus vitamins C+E (HCV). Four weeks later, after sacrifice, the vascular collagen content and MMP-1 protein expression, along with the plasma caseinolytic activity and lipid peroxidation, were measured. MMP-1 was also determined in arterial rings stimulated with native low-density lipoproteins (LDL) isolated from experimental groups. Cholesterol-rich diet augmented plasma lipid peroxidation (P<0.05), reduced the collagen content and increased vascular MMP-1 expression after injury (P<0.05). Enhanced caseinolytic activity (identified as MMP-1) was also observed in HC plasma samples and in supernatants from arterial rings incubated with HC-LDL. Vitamins C and E markedly increased neointimal collagen content (P<0.01), reduced the hypercholesterolemia-induced changes in vascular MMP-1 (P<0.05) and diminished plasma and ex vivo caseinolytic activity. Vitamins C and E may help stabilize atherosclerotic plaque after angioplasty and favor vascular remodeling by increasing collagen content and reducing vascular MMP-1 expression in porcine hypercholesterolemia.
publishDate 2003
dc.date.none.fl_str_mv 2003
2003-01-01
2003
2003-01-01
2012
2012-05-28
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/22270
url https://hdl.handle.net/10171/22270
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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