Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes

Centro de Investigación en Sanidad Animal (CISA)

Detalles Bibliográficos
Autores: Nogales, Aitor, Schotsaert, Michael, Rathnasinghe, Raveen, DeDiego, Marta L., García-Sastre, Adolfo
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/268606
Acceso en línea:http://hdl.handle.net/10261/268606
https://api.elsevier.com/content/abstract/scopus_id/85105213055
Access Level:acceso abierto
Palabra clave:NS1
STAT1
Fluorescent protein
Influenza A virus
Interferon
Luciferase
mCherry
Reporter gene
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spelling Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter GenesNogales, AitorSchotsaert, MichaelRathnasinghe, RaveenDeDiego, Marta L.García-Sastre, AdolfoNS1STAT1Fluorescent proteinInfluenza A virusInterferonLuciferasemCherryReporter geneCentro de Investigación en Sanidad Animal (CISA)The influenza A virus (IAV) is able to infect multiple mammalian and avian species, and in humans IAV is responsible for annual seasonal epidemics and occasional pandemics of respiratory disease with significant health and economic impacts. Studying IAV involves laborious secondary methodologies to identify infected cells. Therefore, to circumvent this requirement, in recent years, multiple replication-competent infectious IAV expressing traceable reporter genes have been developed. These IAVs have been very useful for in vitro and/or in vivo studies of viral replication, identification of neutralizing antibodies or antivirals, and in studies to evaluate vaccine efficacy, among others. In this report, we describe, for the first time, the generation and characterization of two replication-competent influenza A/Puerto Rico/8/1934 H1N1 (PR8) viruses where the viral non-structural protein 1 (NS1) was substituted by the monomeric (m)Cherry fluorescent or the NanoLuc luciferase (Nluc) proteins. The ΔNS1 mCherry was able to replicate in cultured cells and in Signal Transducer and Activator of Transcription 1 (STAT1) deficient mice, although at a lower extent than a wild-type (WT) PR8 virus expressing the same mCherry fluorescent protein (WT mCherry). Notably, expression of either reporter gene (mCherry or Nluc) was detected in infected cells by fluorescent microscopy or luciferase plate readers, respectively. ΔNS1 IAV expressing reporter genes provide a novel approach to better understand the biology and pathogenesis of IAV, and represent an excellent tool to develop new therapeutic approaches against IAV infections.This research was partially funded by the New York Influenza Center of Excellence (NYICE) and the Center for Research on Influenza Pathogenesis (CRIP), two members of the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH), Department of Health and Human Services, Centers of Excellence for Influenza Research and Surveillance (CEIRS) contract No. HHSN272201400005C (NYICE) and HHSN272201400008C (CRIP), and a “Ramon y Cajal” Incorporation grant (RYC-2017) from Spanish Ministry of Science, Innovation and Universities to A.NPeer reviewed16 Pág. This article belongs to the Section Animal VirusesMultidisciplinary Digital Publishing InstituteMinisterio de Ciencia, Innovación y Universidades (España)Nogales, Aitor [0000-0002-2424-7900]Schotsaert, Michael [0000-0003-3156-3132]Rathnasinghe, Raveen [0000-0002-1260-2493]DeDiego, Marta L. [0000-0002-7888-7372]García-Sastre, Adolfo [ 0000-0002-6551-1827 ]Martinez-Sobrido, Luis [ 0000-0001-7084-0804 ]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/268606https://api.elsevier.com/content/abstract/scopus_id/85105213055reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICINN/RYC-2017/Viruseshttps://doi.org/10.3390/v13040698Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2686062026-05-22T06:33:51Z
dc.title.none.fl_str_mv Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
title Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
spellingShingle Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
Nogales, Aitor
NS1
STAT1
Fluorescent protein
Influenza A virus
Interferon
Luciferase
mCherry
Reporter gene
title_short Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
title_full Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
title_fullStr Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
title_full_unstemmed Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
title_sort Replication-Competent ΔNS1 Influenza A Viruses Expressing Reporter Genes
dc.creator.none.fl_str_mv Nogales, Aitor
Schotsaert, Michael
Rathnasinghe, Raveen
DeDiego, Marta L.
García-Sastre, Adolfo
author Nogales, Aitor
author_facet Nogales, Aitor
Schotsaert, Michael
Rathnasinghe, Raveen
DeDiego, Marta L.
García-Sastre, Adolfo
author_role author
author2 Schotsaert, Michael
Rathnasinghe, Raveen
DeDiego, Marta L.
García-Sastre, Adolfo
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia, Innovación y Universidades (España)
Nogales, Aitor [0000-0002-2424-7900]
Schotsaert, Michael [0000-0003-3156-3132]
Rathnasinghe, Raveen [0000-0002-1260-2493]
DeDiego, Marta L. [0000-0002-7888-7372]
García-Sastre, Adolfo [ 0000-0002-6551-1827 ]
Martinez-Sobrido, Luis [ 0000-0001-7084-0804 ]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv NS1
STAT1
Fluorescent protein
Influenza A virus
Interferon
Luciferase
mCherry
Reporter gene
topic NS1
STAT1
Fluorescent protein
Influenza A virus
Interferon
Luciferase
mCherry
Reporter gene
description Centro de Investigación en Sanidad Animal (CISA)
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/268606
https://api.elsevier.com/content/abstract/scopus_id/85105213055
url http://hdl.handle.net/10261/268606
https://api.elsevier.com/content/abstract/scopus_id/85105213055
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MICINN/RYC-2017/
Viruses
https://doi.org/10.3390/v13040698
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812429

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